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- Of 40 random oligonucleotide primers (20 OPC & 20 OPA respectively) for ampliation of Amphioxus genomic DNA, 17 could produce distinctive and reproducible fingerprints varying in length from 200 to 2200 bp. 从40个10bp的寡聚核苷酸随机引物(OPC、OPA各20个)中筛选出17个扩增重复性好，条带清晰、特异性强、数量丰富的引物，对每个个体基因组DNA进行了扩增。 得到RAPD产物的分子量在200-2200bp之间，产物总计127条谱带。
- random oligonucleotide primer 寡核苷酸随机引物
- Objective To investigate the possibility of applying degenerate oligonucleotide primer PCR(DOP-PCR) and comparative genomic hybridization(CGH) in analysing genomic genetics of a single cell. 目的探索单细胞退变寡核甘酸引物PCR(DOP-PCR)-比较基因组杂交(CGH)技术应用于单细胞全基因组分析及着床前胚胎遗传学研究的可能性。
- Random amplified polymorphic DNA was used with 21 arbitrary 10-mer oligonucleotide primers in both New Yangzhou and Leghorn chicken. 13 primers can repeatedly produce RAPD bands clearly. 应用21个10碱基寡聚核苷酸随机引物对新扬州鸡、莱航鸡进行RAPD扩增。
- competitive oligonucleotide primer CCP
- competitive oligonucleotide primer(CCP) 竞争性寡核苷酸引物
- Out of 296 10-mer oligonucleotide primers screened, 25 showed polymorphism among near-isogenic lines. 发现25个引物的扩增产物在近等基因系间表现出特异性。
- THE SELECTION OF OLIGONUCLEOTIDE PRIMER PAIR USEDTO STUDY DLA-DRB1 用于研究DLA－DRB1基因分型的寡核苷酸引物对的选择
- According to known SSUrUNA sequences ot Plasmodium vivax , correlated protozoa and human being, sequences of oligonucleotide primers were defined with computer programming. 根据已知间日疟原虫、其它相关原虫及人小亚单位核糖体核糖核酸(SSUrRNA)基因序列,借助计算机程序分析,设计一对寡聚核苷酸引物。
- Soiled dishes were piled at random. 脏碟子胡乱地堆放着。
- PCR requires the synthesis of oligonucleotide primers complementary to the target DNA. The entire sequence of the target DNA does not need to be known, only the flanking regions. 就整个PCR扩增技术而言，引物的设计占有十分重要的地位，它的优劣直接关系到PCR的特异性与成功与否。
- Methods A pair of oligonucleotide primers were designed with hylA gene as target sequence. Fiftyfour strain of standard LM and 21 strains of irrelevant bacteria were amplified and confirmed by PCR technique. 方法选取hlyA基因作为靶序列设计一对引物，用该引物对54株标准李斯特菌和21株无关菌进行pcr扩增，得到进一步验证。
- Identification of cry-type genesTwo pairs of universal oligonucleotide primers to probe the most conserved regions of all known cryl and cry2 genes were chosen to analyse the nucleotide sequence of 20kb DNA preliminarily. 采用cry1和cry2基因的通用引物对20kb DNA上的核酸序列进行初步分析，结果表明该片段上含有这两类基因，而且cry1基因的拷贝数要低于cry2基因的拷贝数。
- Three pairs of specific oligonucleotide primers for amplifying the NP, P and M gene of NDV were designed according to the published relevant sequences of NDV, and the NP, P and M gene of a virulent NDV strain (Changchun) were amplified by RT-PCR. 计算机辅助设计了3对特异性引物.;应用RT-PCR一次性扩增出NDV长春株NP、P、M基因的氨基酸编码区(ORF)。
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Six pairs of oligonucleotide primers were designed to amplify and sequence scarlet gene of mutant stbr fruit flies which were obtained by outcross between Drosophila melanogaster Bar and wild type fruit flies. 设计了6对引物对棒眼果蝇(Drosophilamelanogaster)Bar与野生型果蝇杂交所产生的亮红眼突变型果蝇stbr的scarlet基因进行了测序;结果在scarlet基因的第4外显子检测到了1个7.;5kb的插入;进一步的测序证实此7
- Ty1-copia-like sequences were amplified from two relative Panax species (Panax ginseng and Panax quinquefolius) genomic DNA, by PCR with degenerate oligonucleotide primers corresponding to highly conserved domains in the Ty1-copia-like retrotransposons. 利用 Ty1/copia-like 类反转座子反转座酶的保守位点设计引物,从人参和西洋参基因组中扩增得到长度为 240bp 左右的 PCR 产物。
- The librarian took a book at random from the shelf. 图书管理员从书架上随便拿了一本书。
- Random mating within a breeding population. 自由交配在一个育种群中自由交配
- Chose a card at random from the deck. 从一副牌中任意选出一张
