您要查找的是不是：
- A prokaryotic expression plasmid was constructed by inserting the BC-48 gene into pGEX-4T-2 vector. Expressed protein induced by IPTG was analyzed by SDS-PAGE and Western-blotting. 构建BC-48的重组pGEX-4T-2表达载体,经IPTG诱导表达后,进行SDS-PAGE、Western-blotting分析。
- prokaryotic expression protein 原核表达蛋白
- Objective:To clone,prokaryotic express and purify APOBEC3G protein in vitro. 目的:原核表达重组APOBEC3G蛋白,为其功能及免疫原性研究奠定基础。
- Objective: To obtain the recombinant augiogenin (Ang) protein by means of prokaryotic expression system and investigate its bioactivity. 目的:利用原核表达系统获得重组血管生成素 (Ang)并研究其生物学活性。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Prokaryotic expression of structural domains of the Newcastle disease virus fusion protein and analyses of their antigenic epitopes. 新城疫病毒F蛋白结构域基因原核表达与抗原表位分析
- This experiment successfully constructed high performance Gly-Gln polymers prokaryotic expression system, whose expression level achieved 40% of total bacterial protein. 本试验成功构建了Gly-Gln多聚体原核表达系统，该系统能够高效表达Gly-Gln多聚体蛋白，表达的目的蛋白占总蛋白的40%25左右。
- The prokaryotic expression strains that efficiently express recombinant human MBL-CLR and the recombinant human MBL-CLR-Trx fusion protein were obtained successfully, which will help the further structure-function research of MBL molecule. 获得可表达人MBL-CLR的大肠杆菌菌株和重组的人MBL-CLR-Trx融合蛋白,为MBL分子结构、功能关系的进一步研究提供了条件。
- Firstly, I subcloned the DNA fragment that encode the four consensus repeat of DAF into the prokaryotic expression vector pET32a(+) which could add a Trx tag on the N-terminal of the target protein. 首先通过PCR的方法，将编码大鼠DAF四个SCR的基因片段亚克隆到Trx融合原核表达载体pET32a(+)。
- To obtain the African horse sickness virus (AHSV) VP7 protein,which is used to prepare serological diagnostic reagent and construct AHS new generation vaccine,the prokaryotic expression is used to express VP7 protein. 为获得非洲马瘟病毒VP7蛋白,以用于制备AHS血清学诊断试剂并为AHS新型疫苗的构建奠定基础,笔者采用原核表达系统表达VP7蛋白。
- Then, a constructed prokaryotic expression plasmid of pGEX-2T-E7 presented by Dr. Werner, was transformed into E. coli BL21(DE3 stain) and induced by IPTG to express GST-E7 fusion protein. 为了研究HPV16E7疫苗的需要，我们利用Werner博士惠赠的pGEX-2T-E7原核表达质粒转化入大肠扪：菌BL21(DE3)中，用IPTG诱导后，裂解细胞，用SDS-PAGE鉴定诱导效果，发现在43kDa处有融合蛋白的表达。
- Studies on Prokaryotic Expression of Plant Anti-freeze and Salt Tolerance Genes. 盐基因原核表达研究。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective: 1.Subclone B7.2(IgV+C),IgV and IgC domains and construct prokaryotic expression system of them. 2. 目 的： 1．亚克隆B7.;2分子的胞外(IgV+C);IgV及IgC各区段并构建其原核表达载体。
- These results suggest that the prokaryotic expressed AGT protein is an effective immunogen for the preparation of anti-AGT antiserum . 以上结果提示，利用大肠杆菌融合表达的AGT蛋白可有效刺激家兔产生AGT抗体。
- SDS PAGE analysis indicated that the expressed protein was about 30 kD. SDS PAGE分析 ,表达出约 30kD大小的蛋白 ;
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- The result of SDS-PAGE indicated that the expression protein was about 40KD and reached to its peak at about 5 hours after inducing. SDS-PAGE电泳显示;表达的融合蛋白的分子量为40KD左右;且在诱导表达5小时后表达量最多;大约占菌体总蛋白的22.;4%25。
- I have studied the technology of molecular biology, protein prokaryotic expressing, refolding in vitro, purification, identification and MHC tetramer. 我广泛查阅中外文文献，出色完成研究课题，为导师的国家自然科学基金及科技部973计划资助的课题奠定基础。
- Among the identified differential expression protein,the expression of DJ-1 protein was decreased in DADS-treated group. 与未处理组比较,初步鉴定的差异蛋白质DJ-1蛋白在处理组中表达下调,其功能与转录调节有关。
