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- As the most efficient strategy in gene clone, positional cloning has been used widely in QTL cloning in rice. 图位克隆作为基因克隆的最有效手段, 在水稻QTL克隆方面取得了很大的进展。
- Otherwise, the nodus of QTL positional cloning and the corresponding solving methods were discussed. 文章还对QTL图位克隆的难点及解决方法进行了有益的探讨。
- Thin recombinant with the cDNA ~crt fragment was released frc the positive clone and was di ?z; tcJ with EcoRI; the eDNA fra, ent was 2kb in length. 释放质粒后，进行酶切鉴定，该阳性克隆cDNA插入片段的大小为2kb左右。
- Zhang YY, Proenca R, Maffei M, et al. Positional cloning of the mouse obese gene and it's human homdogue[J]. Nature,1994, 372,425-432. 汪丽惠，许广润，张树基，等．现代内科诊疗手册[M]．北京医科大学、中国协和医科大学联合出版社，1994．507
- Objective To investigate the causative genes of the kindred with genetic hearing impairment by means of positional cloning approach. 摘要目的探讨遗传性聋基因的定位克隆研究。
- Blast analysis displayed one of the Northern blot positive clone is homologous with autotaxin gene, and another is homologous with calcyclin gene. 对这13个克隆进行测序和Blast同源序列分析表明，其中一个Northern杂交阳性克隆与autotaxin基因同源，另一个与钙周期蛋白基因同源。
- The resu1ts of MCT1 gene ani-sense reconsmicted vector transfectionand identification: after being transfected, A549 cells were screened byG4l$,and positive cloning was obtained. （1）MCT1基因反义重组载体转染、鉴定分析结果：反义基因转染A549细胞后，经G418筛选，出现阳性克隆；
- Methods:Screening peptide library with EAN serum. Identified positive clone by ELISA and compared similarity between positive clone and LPS of Campylobacter jejuni. 方法 :采用噬菌体呈现技术对 8个EAN模型血清抗体进行肽库筛选 ,并将获得的克隆用ELISA方法进行阳性鉴定 ,以及将阳性克隆与空肠弯曲菌LPS相似性进行比较。
- The wood is held in position by a clamp. 木头用钳夹固定住了。
- The recombinant plasmid was transformed into the yeast strain S. cerevisiae BJ1990 to carry out the primary expression experiment. In culture supernant of screened positive clone, the hirudin activity was detected to be 30 ATU/ml. 用正确的重组表达质粒转入酿酒酵母BJ1990细胞进行了初步表达实验，在筛选出的阳性克隆的培养上清中检测出的水蛭素活性为30ATU/ml，表达量达4mg/L。
- The girl is quite suited for the position [work]. 这姑娘很适合担任这个职务[这个工作]。
- I can live in clover when I get that position. 我得到那个职位便可过上优裕的生活。
- MBD1 siRNAs had beensuccessfully integrated into the plasmid. MBD1 siRNAs vector was transfected intopancreatic cancer cell AsPC-1 by liposome. Positive clone was obtained by the screenof G-418. 采用脂 质 体 介 导 的 方 法 将 MBD1siRNAs 表 达 质 粒 转 染 胰 腺 癌 细 胞 系AsPC-1,通过 G-418 的筛选获得稳定表达 MBD1siRNAs 的阳性克隆。
- He bribes his leader to consolidate his position. 他贿赂他的领导来巩固他的地位。
- In our studying, we not only used the CAPC data in positional clone, but also developed about 30 novel makers based on CAPC and new protocol of heterogeneous double helix analysis for SNPs or small InDels. 我们不仅利用CAPC数据资源进行定位,而且还发展了近30个新分子标记,在分子标记的检测方面发展了异源双螺旋分析技术。
- In this study, the dicistronic expression vector (pEGFP-C1) was used to be transfected into human lung cancer cell line(ASTC-a-1)and a positive clone which stably expressed GFP in high level was obtained. 利用常规转染方法将带有EGFP基因的质粒载体导入人肺腺癌肿瘤细胞 (ASTC a 1) ,并得到GFP稳定表达的细胞株。
- He took up a radical position on this issue. 他对这个问题抱着激进的态度。
- QTL mapping opens up broad prospects for marker-assisted selection of complex quantitative disease resistance characters and the positional cloning of quantitative resistance genes. 展望了QTL作图对复杂的数量抗病性的标记辅助选育和数量抗性基因图位克隆的发展前景。
- She is unfit for such a senior position. 她不胜任这种高级职务。
- They plotted the new position of each vessel. 他们标明每艘船的新位置。
