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- pmkaryotic expression plasmid 原核表达质粒
- In addition, a set of expression plasmid vectors, PMS-31b. 同时构建一组质粒表达载体PMS-31b。
- Constructed the expression plasmid pTrc-rCR and checked it. 构建表达质粒pTrc-rCR,并酶切检验;
- Objective To clone the human angiotensin-converting enzyme 2 (ACE2)and construct its eukaryotic expression plasmid. 目的克隆人血管紧张素转换酶2基因(ACE2),并构建其真核表达载体。
- Results: Recombinant expression plasmid of ALR was confirmed correct by restriction enzyme digestion and sequencing. 结果：酶切鉴定及测序结果提示表达产物正确；
- Results The bicistronic eukaryotic expression plasmid was corrected and can co - express human BMP -2 and VEGF165 mRNA in vitro. 该重组质粒能在体外同时表达BMP2及VEGF165 mRNA。
- The co expression plasmid encoding FasL and VEGF165 named pCI FasL IRES VEGF165 (simplified as pCI FIV) was successfully constructed. 再以此为基础构建得到编码FasL和VEGF165的共表达质粒 pCI FasL IRES VEGF165 (简称pCI FIV )。
- Expression and purification of recombinant RTAsThe expression plasmid Pkk223.3-RTA was introduced into E. coli JM 109 by CaCl2-mediated method. 将构建好的重组质粒pKK223.;3-RTA和pKK223
- The experiment results covered several following points:(1) adw was cloned into nuclear plasmid PB, PBG and chloroplast expression plasmid PLCTB . 将乙肝表面抗原基因(adw型)与细胞核载体PB、PBG以及叶绿体表达载体PLCTB进行定向克隆，经PCR和测序鉴定都获得了重组子，分别命名为PB-adw、PBG-adw、PLCTB-adw。
- AIM: To construct pET32a/His MBL-CLR recombinant prokaryotic expression plasmid and to express mannan-binding lectin-CLR (MBL-CLR) protein in E. 目的:在大肠杆菌中表达人甘露聚糖结合凝集素(MBL)胶原样区(CLR)蛋白。
- Methods: 1.The KK gene was directional cloned into the pAAV-MCS. Thisrecombinant pAAV expression plasmid was called pAAV-KK. 方法:1.;将KK 基因定向克隆入腺相关病毒载体质粒pAAV-MCS 中构建成pAAV-KK。
- To construct mammal expression plasmid pcDNA 3.1 ( + )/GDF-5 and check the expression of it in bone marrow mesenchyal stem cells of mice. 目的:通过基因重组技术体外构建真核表达质粒pcDNA3.;1(+)/GDF-5;并检测其在小鼠骨髓基质干细胞中的表达。
- Methods:The epf gene fragment was amplified by PCR from ZYH24 and cloned into prokaryotic expression plasmid pGEX4T-2 to form pGEX4T-2-epf. 方法根据GenBank S.;suis2epf基因序列设计引物;克隆ZYH24株epf基因片段并进行序列分析;
- Then the BLY cDNA fragment was subcloned into prokaryotic expression vector pGEX-4T-1, forming the prokaryotic expression plasmid (pG-BLY). 克隆PCR产物，并构建了pGEX-4T-1-BLY原核表达载体。 经BamHI和EcoRI酶切及质粒PCR鉴定，证实本实验构建的新型牛溶菌酶基因已克隆到原核表达载体pGEX-4T-1上，为进一步研究其诱导表达条件及生物学功能奠定了基础。
- The customers should provide the gene expression plasmid which has been done the fluorescence labelling. We will provide the detail reports and microscopical photos. 技术服务完成后向客户提供详细的实验报告和荧光共聚焦显微镜照片。
- The expression plasmid pSV-F was constructed by inserting the F gene into the pVAX1 vector, and transfected into the cultured COS 7 cell line via liposomes. 将HeB02株F基因插入真核表达载体pVAX1中,构建了真核表达质粒pSV-F,通过脂质体转染COS-7细胞,SDS-PAGE分析可见表达的特异蛋白条带;
- Methods: The specific BDNF sequence was cloned into the plasmid of pAAV-MCS in AAV helper-Free system to construct the BDNF expression plasmid pAAV-MCS-BDNF. 方法应用基因克隆技术将大鼠BDNF的cDNA基因序列克隆入腺病毒质粒pAAV-MCS,PCR酶切测序鉴定序列。
- Methods:(CTP)_4coding gene from pBSMR-(CTP)_4 was cloned into prokaryotic expression vector pET-28a(+) and the expression plasmid pET-28a(+)- (CTP)_4was obtained. 方法:将pBSMR-(CTP)_4中的(CTP)_4基因片断克隆入表达载体pET-28a(+),得到表达质粒pET-28a(+)-(CTP)_4。
- The identified cDNA was cloned into the new type of prokaryotic expression vector pQE-80L, and a synthetized expression plasmid named pQE-80L/DHFR/ABP obtained. The E. 将此基因克隆到原核表达载体pQE-80L,获得融合表达质粒pQE-80L/DHFR/ABP,在1%25IPTG诱导下进行表达。
- Cotransfection of GalT I promoter /luciferase reporter and Ets-1 expression plasmid increased the luciferase reporter activity in a dose dependent manner. 我们采用荧光素酶报告基因的方法证实Ets-1可激活 GalT I报告基因的表达，并具有量效关系。
