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- Methods Using carrier c onstruction , plasmid transfection and identificaion,the PEMT-2 overexpression cells was obtained, and protein content and proliferation of cell were analysed by ce ll culture and counts and Coomassie brilliant blue assay. 方法 采用载体构建、质粒转染和鉴定方法 ,得到PEMT 2过表达细胞 ,用细胞培养、计数及考马斯亮蓝法分析大鼠肝癌细胞与PEMT 2过表达细胞蛋白含量及增殖情况。
- Keywords Amelogenin Recombinant plasmid Transfection Expression; 釉原蛋白;重组质粒;转染;基因表达;
- Abstract： Objective： To compare PGD210 plasmid transfected dendritic cells with untransfected dendritic cells in activating T cells to kill the K562 cells. 目的： 比较PGD210转染的DC与未经转染的DC，刺激T细胞诱发免疫应答，有效激发T细胞并杀伤K562细胞的功能。
- Both RT PCR and immunofluorescent staining of recombinant plasmid transfected COS 7 showed positive reaction, thus indicating that the recombinant plasmid pcDNA3 LACK can express LACK protein in euka ryotic cell COS 7. 实验结果显示转染了重组质粒的 COS- 7细胞 ,其 RT- PCR及免疫荧光检测均呈阳性反应 ,证实重组质粒 pc DNA3-L ACK能在 COS- 7细胞中有效表达 L ACK蛋白。
- plasmid transfection 质粒转染
- Methods:The plasmid pcDNA3 containing the wild type p16 gene and neo gene was used in the transfection experiment. 方法：采用pcDNA3质粒进行转染，该质粒含有野生型P16基因片段和新霉素抗性基因。
- Methods:The HUVEC over-expressing EL(named HUVEC-EL) was got through transfection HUVEC with plasmid pcDNA4-EL-myc/His. 方法:通过质粒转染法使HUVEC高表达EL(HUVE-EL),并与正常HUVEC对照,观察高表达EL对HUVEC的增殖、迁移和凋亡的影响。
- TNA may enhance transfection rate of plasmid DNA mediated with liposome, and may be beneficial to the treatment of cancer. TNA可促进脂质体介导质粒DNA转染结直肠癌细胞 ,将营养支持与基因治疗相结合 ,有望提高对肿瘤患者支持治疗效果。
- The enhanced green fluorescent protein(EGFP) plasmid was used as positive control and the transfection efficiency was detected by flow cytometry. 同时转染带有增强型绿色荧光蛋白(EGFP)的载体作为阳性对照,流式细胞术检测其绿色荧光以确定转染效率;
- The plasmid pBV220-Arr. could be expressed in E. 成功构建的重组质粒pBV220-Arr在E.
- The recombinant plasmid pUC18 E6 had been got. 获得重组质粒 pUC18 E6。
- METHODS:H1299(human non-small cell lung cancer cell line)cells were co-transfected with recombinant plasmid pEF-BOS/GST-EGFR-TKD and pBabe-puro by calcium phosphate transfection. 方法:采用磷酸钙共沉淀基因转染技术,将已构建的pEF-BOS/GST-EGFR-TKD质粒DNA与pBabe-puro质粒DNA共同导入体外培养的人非小细胞肺癌细胞系H1299中。
- Keywords Gene;neurofibromatosis 2 Polymerase chain reaction Plasmids Transfection Gene expression; 基因;神经纤维瘤病2型;聚合酶链反应;质粒;转染;基因表达;
- Conclusion The transfection of the GM-CSF plasmid in vivo could stimulate the proliferation of the pre DC of bone marrow, and enhance antigen presentation of DC. 结论GM-CSF质粒体内注射可以明显刺激诱导小鼠髓源性DC前体的增殖，增强DC的抗原提呈作用。
- Results The constructed recombinant plasmid contained the sequence of hOPG gene.After transfection with the plasmid, active OPG protein could be expressed in C2C12 cells. 结果 经酶切和测序鉴定证实本实验构建的重组表达质粒正确，该质粒在体外转染C2C12细胞后可表达功能性OPG蛋白。
- Result: The pcDNA3.1-VNTR plasmid transfected dendritic cells could express VNTR in vitro; 结果： pcDNA3.;1-VNTR质粒转染的树突细胞可以表达MUC1-VNTR多肽；
- After ATF gene transfection, the ATF mRNA was obviously expressed. 结果表明,转染ATF基因后,肿瘤细胞呈现ATF基因的明显表达;
- Transfection, make stable C/EBP p expression cell line. 质粒转染，制备稳定表达C尼BPp的细胞系。
- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- Gene transfection is the key technique of gene therapy. 摘要基因治疗的关键技术是基因转移。
