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- Objective To construct the plasmid expressing BDV p40 gene. 目的构建博尔纳病病毒p40基因重组表达质粒。
- The plasmid expression vector aiming directly at cerb-B1 gene might suppress cerb-B1 gene and EGFR expression in human colon cancer LoVo cells. The new therapeutic modalities could be used in the treatment for human colon cancer. 说明本研究构建的针对cerb-B1基因的质粒表达载体可以显著抑制cerb-B1基因和EGFR蛋白在人结肠癌LoVo细胞的表达,为结肠癌的基因治疗提供了新的思路。
- Objective To construct the prokaryotic expression plasmid expressing woodchuck hepatitis virus core antigen(WHcAg) and prepare polyclonal antibodies. 目的构建土拨鼠肝炎病毒核心蛋白质粒并进行原核表达、抗体制备。
- Methods pCR3.1/GR plasmid expressing gastrin receptor CCK 2R was transfected into colonic carcinoma cell line CoLo320 by Lipofectamine~(TM) 2000 and expressing stably CCK 2R clones was screened by G418.The expression levels of gastrin receptor of Coi. 方法脂质体转染表达胃泌素受体CCK-2R的pCR3.;1/GR质粒于结肠癌细胞CoLo320中;G418筛选出稳定表达CCK-2R的阳性克隆;RT-PCR鉴定;转染成功命为CoLo320WT。
- Lactococcus plasmid expression vector pNZ2103 乳酸菌质粒表达载体pNZ2103
- Results The anti-carcinoma ScFv immunotox recombinant plasmid expressed the ScFv immunotoxin in inclusion.Its molecular weight was about 66KD. 结果诱导表达了约66KD的蛋白，分子量符合预计大小，以包涵体的形式存在于菌体中。
- The recombinant bacterium harboring the recombinant plasmid expressed L-asparaginase II in high level as inclusion bodies. 通过对包涵体的提取与纯化，结合离子交换层析获得天门冬酰胺酶II的蛋白质纯品。
- After induced by IPTG, the recombine plasmid expressed the whole rat IDE and purified by the thrombin affinity chromatography. 重组质粒经IPTG诱导后，表达出了全长的大鼠IDE，并经过凝血酶层析柱进行纯化。
- The serum from anemic rats treated with naked plasmid expressing human EPO showed obvious neutralizing activity to inhibit the proliferation of BEF-2 cells. 在大鼠模型中,表达人EPO基因裸质粒组的血清有明显的中和活性,可抑制BEF-2细胞的增殖。
- A73expressed at lower levels in B95.8and Raji th an in SUNE1.The constructed recombinant plasmid expressed stably in HEK293c ells,in which the fusion protein was found to be mainly cytoplasmic. 鼻咽癌外周血淋巴细胞中未检测到A73表达;SUNE1细胞中A73的表达高于B95.;8和Raji细胞。
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- He always shows a doleful expression. 他总是露出忧郁的表情。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
- She looked at me without expression. 她毫无表情地朝我看着。
- The scenery there was beautiful beyond expression. 那儿的风景美丽得无法形容。
- He looked at her with a solemn expression. 他表情严肃地望着她。
- There was an expression of discontent on her face. 她脸上有不满的表情。
- People have the rights of assembly and expression. 人们有集会和发表言论的权利。
- She gave expression to her sadness. 她流露出了悲伤之情。
