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- To obtain M13 phages displayed antigenic epitope recognized by BAC5 McAb,a kind of monoclonal antibody located on poorly or undifferentiated squamous carcinoma cells. 获得可递呈抗低分化和未分化癌细胞单克隆抗体(BAC5单抗)抗原表位的M13噬菌体克隆系。
- T1 phage displayed a higher survival than poliovirus. T1噬菌体的存活高于脊髓灰质炎病毒。
- Objective To screen two MUC1 antigen mimic epitopes by phage display peptide library technology. 目的从噬菌体表面展示的随机12肽库中筛选出MUC1抗原的模拟表位。
- Objective To select minotope of NMDAR1 by using phage displayed random peptide library. 目的利用噬菌体展示随机肽库技术,进行NMDAR1抗原表位研究。
- Ke Shen, et al. Bio-panning of HGV antigen epitope from a phage display library. Progress in Natural Science. 2000;10(6):446-450. 柯屾,等。核糖体展示技术。见:分子文库-中国生物技术丛书。沈倍奋主编。2001。北京:科学出版社。
- To study the common epitope between Lumbricus terrestris( Lt) and Schistosoma japonicum( Sj) by phage displayed technology. 目的采用噬菌体呈现技术,获得蚯蚓与血吸虫的共同表位。
- Objective To identify the B-cell epitopes of dengue virus type 2 (DEN2) envelope glycoprotein (E) from a random phage displayed peptide library (RPL) using a serotype-specific monoclonal antibody (McAb) of DEN2 NGC strain. 目的 用噬菌体展示肽库筛选登革 2型病毒 (DEN2 )E蛋白的抗原表位 ,并确定该抗原表位性质。
- In recent years, phage display was considered for vaccine development. 近年来,呈现噬菌体开始应用于疫苗的研究。
- Phage display is a new recently developed technique,in which an exogenous peptide or protein is fused with capsid protein phage and displayed on the surface of it. 噬菌体展示技术是新近发展起来的将外源肽或蛋白与特定噬菌体衣壳蛋白融合,并展示于噬菌体表面的一项新技术。
- phage displayed random peptide library 噬菌体随机肽库
- Here, the basic mechanism and the application of phage display random peptide libraries are briefly introduced. 本文简介此类文库的基本作用原理及应用。
- Objective To screen polypeptides binding to human CD137 by biopanning phage display random heptapeptide library. 目的应用噬菌体随机七肽库筛选与人CD137特异结合的肽序列。
- Aim The phage display antibody library was constructed to obtain functional anti Fas Fab. 目的构建噬菌体抗体库,获得具有功能的抗Fas?Fab噬菌体抗体。
- Conclusions:E7 protein of HPV16 (HB strain) could be prepared by gene clone technique.HB-E7 protein displayed antigenicity of standard E7 protein. 结论:HPV16湖北株E7蛋白可通过基因克隆方法制备,并保留了标准E7蛋白的抗原性。
- Objective To search for new antigens expressed on the surface of thymic stromal cells using phage display technology. 目的利用噬菌体展示技术寻找胸腺基质细胞表面的新抗原。
- Phage display random peptide library is a kind of polypeptide library developed by filamentous bacteriophages. 摘要噬菌体随机展示肽库是一类利用丝状噬菌体发展起来的多肽文库。
- Phage display technology is an ideal approach to the study on the protein evolution. 噬菌体展示肽库技术是研究蛋白质分子进化的理想工具。
- With the development of phage display libraries, it might be applied extensively in many fields of research. 随着此项技术的不断完善和发展,噬菌体肽库已在许多领域得到了广泛的运用。
- Methods mRNA was extracted from lymphocytes of a volunteer with high level antibody to HBsAg. Fab phage display library was constructed. 方法 采用噬菌体抗体库技术 ,从乙肝疫苗免疫后健康人外周血淋巴细胞中提取抗体基因建立抗体库 ,用纯化的HBsAg筛选Fab抗体 ;
- Conclusion The phage display technique can be applied to study the viral antigenic peptides with the advantages of simple, accuracy and rapidity. 结论噬菌体展示技术完全可应用于病毒蛋白抗原序列的筛选,并具有简便、快速、准确的优点。