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- oncolytic adenovirus vector 溶瘤腺病毒载体
- It is, therefore, feasible to exploit IL-8 promoter to control the replication of oncolytic adenovirus aiming to achieve more tumor-targeted oncolysis for treating NSCLC. 因此，在非小细胞肺癌中以介白素八调控子控制溶瘤腺病毒应可具有更佳的肿瘤专一性与溶瘤效果。
- Objective To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell. 目的探讨用细菌内同源重组法制备含TK自杀基因腺病毒对肝癌细胞的杀伤作用。
- The dispersed pancreas cells were transfected with exogenous gene by adenovirus vector and cationic polymer reagent, respectively. 在细胞分散后对孔鳐胰脏细胞用腺病毒载体和阳高子多聚物进行外源基因的转染。
- The results showed that: adenovirus vector in HEK-293 cells in the packaging successful, the virus appears macrophages spot. 2. 最后用磷酸钙转染法把重组腺病毒载体转染到 HEK-293细胞中包装。
- Results: Adeno-X-SSTR2,recombinant adenovirus vector,can infect pancreatic carcinoma cells MIA-PaCa-2 and AsPC-1 efficiently,which both can express SSTR2 mRNA and their protein. 在不同浓度NC-8-12和奥曲肽作用下;未转染SSTR2基因胰腺癌细胞株的Survivin表达量无明显变化(P>0.;05);转染了SSTR2基因胰腺癌细胞株的Survivin表达量明显下调(P<0
- Results Through direct infection and MTT assay,we found that adenovirus vector containing angiostatin K1-5 could inhibit the proliferation of human vascular endothelial cells. 结果直接感染台盼蓝染色排除法及MTT法检测表明,携带血管抑素K1-5基因的腺病毒能显著抑制血管内皮细胞增生,并对其具有杀伤力。
- "The failure of the Merck vaccine up on behalf of the non-replicating adenovirus vector vaccine failure, not on behalf of other AIDS vaccines will be no hope. “默克疫苗的失败至多代表非复制型腺病毒载体疫苗的失败，决不代表其他艾滋病疫苗就没有希望。”
- Objective To investigate the suppression effects of a adenovirus vector containing angiostatin K1-5 gene to the proliferation and migration of vascular endothelial cells. 目的研究携带血管抑素K1-5基因的腺病毒载体对其对血管内皮细胞增殖的抑制作用。
- Abstract:Objective:To construct the recombinant adenovirus vector carrying rat vascular endothelial growth factor (VEGF) as preparation for later use for genetic transfection. 目的:探讨构建携带大鼠血管内皮细胞生长因子(VEGF)的重组腺病毒载体方法，为后续的基因转染研究作准备。
- With the development of adenovirus vector, the adenovirus will play a more and more important role in controlling human and animal diseases, especially viral diseases as well as in human gene therapy. 随着动物腺病毒载体的发展，它将在控制人和动物传染性疾病，特别是病毒性传染病以及人类的基因治疗中发挥越来越重要的作用。
- Objctive:It is surveyed whether the recombinant adenovirus vector pAdeno-X-siFas 1+siFas 2 expressing two Fas-targeted short hairpin RNAs(shRNA) could supress the overexpression of Fas induced by LPS/D-GalN in BALB/c mouse liver. 目的:观察串联表达Fas-shRNA的腺病毒载体pAdeno-X-siFas 1+siFas 2对脂多糖(LPS)/D-氨基半乳糖(D-GalN)诱导BALB/c鼠肝细胞Fas过表达的影响。
- Successful construction of the recombinant adenovirus vector containing the Ki67-siRNA gene was achieved and it can be used to significantly inhibit expression of the Ki67 gene and the growth human colon carcinoma cells. 含有Ki67-siRNA的重组腺病毒构建成功,其能抑制人结肠癌细胞Ki67基因表达和生长。
- Keywords Cancer;Biotherapy;Oncolytic adenovirus;Heating;Abscopal effect; 肿瘤;生物治疗;溶瘤腺病毒;热疗;远端效应;
- Keywords oncolytic adenovirus;gene therapy;hepatocellular carcinoma; 裂解型腺病毒;基因治疗;肝细胞肝癌;
- The recombinant adenovirus vector, pAdCMV-IL3-Endo, was cotransfected into 293 cells together with pBHG10 by lipofectamine, and recombinant adenovirus, which named Ad-ILs-Endo, were generated by homologous recombination. 然后用脂质体法将上述穿梭质粒与质粒pBHG10共转染293细胞，经同源重组获得复制缺陷型重组腺病毒Ad-IL_3-Endo。
- Keywords KAI1 gene;Adenovirus vector;Gene therapy; KAI1基因;腺病毒载体;基因治疗;
- Conclusions Lentivirus vectors have more advantages as a gene transferring (gene conduction) system than adenovirus vectors. 结论慢病毒载体较腺病毒载体作为基因转导系统更有优势。
- The construction of recombinant adenoviral vector containing gene of EWS-FLI1 and its expression in PBMC . 介导尤文肉瘤融合基因的重组腺病毒的构建及其在外周血单个核细胞(PBMC)中的表达
- Methods rVSMCs were transfected with an adenoviral vector expressing Mfn2(Adv-Mfn2-GFP). 方法体外培养rVSMCs,感染含有Mfn2基因的腺病毒载体(Adv-Mfn2-GFP)。
