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- Samples were fixed for histology, and gene expression was assessed with a dual-dye 22 000 oligonucleotide microarray. 样品组织学固定,基因表达用双染的22000寡核苷酸微点阵评估。
- Oligonucleotide microarray technology is a powerful data-mining platform and has been widely applied in biosciences. 摘要寡核苷酸芯片技术是一种高通量发掘和采集生物信息的强大技术平合,目前已广泛应用于生物科学领域。
- On the basis of the first step of amplifying PCR, it has been hybridized with oligonucleotide microarray in order to analyze whether the amplified PCR product possess antimicrobial agent resistance or not. 摘要在第一步耐药性靶基因得到扩增的基础上,通过与芯片进行杂交来分析所扩增的PCR产物是否是已经发生了突变而能导致抗药性的基因。
- Gene expression profiles of SW1990, SW1990/R, ASPC and ASPC/R cells were screened using oligonucleotide microarray (Affymetrix HG U133 2.0plus) which contained approximately 38000 human genes. 应用基因芯片技术检测胰腺癌细胞株SW1990、ASPC及耐放射细胞亚株SW1990/R、ASPC/R的基因表达谱。 在47400个转录本中,通过Two way ANOVA分析得到与放射敏感性相关的统计学差异的基因共2392条,筛选亲本株与耐放射细胞亚株放射敏感性相关的差异在两倍以上的共同差异基因137条。
- To improve the performance of assays on the oligonucleotide microarray, the factors that influence the hybridization effects such as surface chemistry, probe size, spacer length, hybridization conditions etc were intensely studied and optimized. 为改善寡核苷酸芯片的分析性能,对影响芯片杂交结果的因素,如片基表面的化学处理、探针的长度、间隔臂的长度、杂交条件等,进行了深入的研究和优化。
- Evaluation of Two Different Sample Labeling Methods on Background Signal Intensities for 60 mer Oligonucleotide Microarrays 两种标记方法对60mer寡核苷酸芯片背景信号影响的初步分析
- oligonucleotide microarrays 寡核苷酸芯片
- Keywords Pancreatic neoplasms;Oligonucleotide microarray;Gene expression; 胰腺肿癌;寡核苷酸芯片;基因表达;
- Calculate the distibution of the melting temperature of the oligonucleotide probe sets that Affymetrix uses for its microarrays. 计算用于微阵列的基因表达谱、和基因分型研究技术平台使用的、低聚核苷酸探针装置的溶解温度分布。
- Expression microarrays are often constructed by immobilizing probes of cDNA and oligonucleotide on two-dimensional modified glass slides. 通常表达谱芯片是将DNA固定在玻璃载体的固液表面,其DNA的动力学行为受多种因素影响,而杂交动力学行为影响的杂交效率是DNA动力学的主要方面,直接影响DNA芯片的信号强度。
- Human nonmethylated oligonucleotide,NON Elisa ... 人非甲基化寡核苷酸(NON)试剂盒;
- DNA microarrays and gene expression :P. 鲍尔迪; G.;W
- Preparation Optimization and Properties of the Aldehyde Microscopic Slides for Oligonucleotide Microarray Fabrication 用于寡核苷酸芯片制备载体的醛基载玻片的制备方法优化及性质研究
- Design and preparation of oligonucleotide microarray for vaccinia virus detection 痘苗病毒寡核苷酸检测芯片的设计及研制
- Construction and preliminary application of oligonucleotide microarray specialized for pancreatic adenocarcinoma 胰腺癌相关基因寡核苷酸芯片的制备和初步应用
- The impact of three fluorescence marker methods to oligonucleotide microarray hybridizesignal 三种荧光标记法对寡核苷酸芯片杂交信号的影响
- Development and Application of Oligonucleotide Microarray for Detection of SARS Coronavirus SARS冠状病毒检测寡核苷酸基因芯片的研制及应用
- Keywords physical restraint;adrenocorticotropic hormone;corticosterone;post-stress recovery;oligonucleotide microarray; 束缚应激;促肾上腺皮质激素;皮质酮;应激后恢复;基因芯片;
- The pilot study on development of lamivudine-resistance oligonucleotide microarray and its reliability in clinical application 检测拉米夫定耐药位点基因芯片的研制及其应用初探
- A Robust and Low-cost Substrate for Oligonucleotide Microarray Compared with CMT-GAPSTM Aminosilane Slide and Microscope Slide 三种寡核苷酸芯片片基的比较