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- Abstract:T he principle and methods of plant microdissection and microcloning are introduced in detail, and their applications in the field of botany are summarized. 摘要:本文综述植物染色体微切割、微克隆技术的原理和方法,以及该技术在植物学研究上的应用。
- Chromosome microdissection and microcloning technology was first used in animal and human. With the development of PCR,they was applied in plant genetic and evolutional researches widely. 染色体微切割与微克隆技术首先在动物和人类上得到应用,随着PCR技术的发展,该技术在植物遗传与进化研究等方面也得到了较为广泛应用。
- Microdissection and microclone are new techniques of molecularbiology.It plays a very important roles for marking and isolationing of gene,repeat tissue of DNA library,making of genetic map. 微切割、微克隆是分子生物学的一项新技术,它对于基因的标记与分离、DNA文库的重组及遗传图谱的绘制都发挥着重要作用。
- Identification of Ditelosome 7Ai-1L of the Chromosome Arm in Thinopyrum intermedium by Cytogenetic Methods and its Microdissection and Microcloning 中间偃麦草染色体臂7Ai-1L端体的细胞遗传学鉴定及显微分离与克隆
- microclone (显)微克隆
- microcloning 微克隆
- DNA microcloning DNA的微克隆
- chromosome microcloning 染色体微克隆