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- methylotrophic Pichia pastoris 毕赤酵母
- Objective:To express ancrod,a snake venom thrombin-like enzyme,in methylotrophic yeast Pichia pastoris. 目的:利用毕赤酵母表达具有生物学活性的蛇毒类凝血酶安克洛。
- The methylotrophic yeast Pichia pastoris is now one of the important tools used in molecular biology for the generation of recombinant protein. 巴斯德毕赤酵母目前已经成为分子生物学研究中，表达外源蛋白的一种重要工具。
- Qiu D R,Zhu J P,Wang L,et al.Expression of recombinant human p53 in methylotrophic yeast pichia pastoris[J].Journal of Biotechnology,1999,15(4):477-481. [8]邱荣德;朱建蓓;王垒;等.;人p53蛋白在巴氏德毕赤酵母中的表达[J]
- The ScFvA1-Etag and the glucose-linker-ScFvA1 (GLS) fusion gene were cloned into the vector pPIC9K and expressed in methylotrophic yeast Pichia pastoris GS115, respectively, under the control of the AOX1 promoter. 将ScFvA1-Etag基因，GLS融合基因重组进大肠杆菌-酵母穿梭质粒pPIC9K, 分别构建含有ScFvA1-Etag基因，GLS融合基因的表达载体pPIC9K-ScFvA1-Etag，pPIC9K-GLS。
- Aim To construct the Pichia pastoris expression system of RS gene. 目的建立白藜芦醇合酶基因的毕赤酵母表达系统。
- Methylotrophic Yeast (Pichia pastoris) 甲醇酵母
- Methylotrophic yeast Pichia pastoris 毕赤甲醇酵母
- In Basal Salts Medium, the yeast grows up to A600=76 inoculated 10% seed pichia pastoris with A600=8-12 in 24h. 在无机盐培养基中,按10%25接种A600=8-12的种菌后,24小时酵母菌增殖至A600=76;
- AIM: To express human anti-keratin Fab in Pichia pastoris secretively and optimize the expression condition. 目的:用巴氏毕赤酵母表达抗角蛋白抗体Fab段并优化表达条件。
- The HRPC3 gene was expressed successfully by using Pichia pastoris as host strain and the activity of it is 56 IU/L. 应用毕赤酵母作为受体菌,成功表达了HRPC3基因,其活性为56 IU/L。
- By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115. 对重组酵母进行诱导表达,SDS-PAGE和Western印迹分析,结果表明cGH在毕赤巴斯德酵母GS115菌株中获得了高效表达。
- Pichia pastoris is one of the most used expression systems for the high level expression of heterologous proteins. 巴斯德毕赤酵母是目前应用最广泛的外源蛋白表达系统。
- Pichia pastoris expression system is a recently developed system to efficiently express foreign protein. 摘要毕赤酵母表达系统是近年来发展起来的一种高效表达外源基因的表达系统。
- Cai R.Study on the Production of S-Adenosylmethionine by Recombinant Pichia pastoris. [4]蔡瑞.;从重组毕赤酵母中制备腺苷甲硫氨酸的研究
- The two-step fermentation conditions of opioid peptide expressed in Pichia pastoris were studied. 研究了由毕赤酵母胞内两步发酵法表达活性小分子阿片肽的条件。
- The optimal fermentation conditions for production of xylanase by engineered Pichia pastoris GS115-ATx in shake-flask cultivation was investigated. 对基因工程菌Pichia pastorisGS115-ATx在摇瓶发酵水平产木聚糖酶条件进行了优化.
- And then , we urged Saccharomyces cerevisiae and Pichia pastoris to fuse by means of isolation of haploid and protoplasts fusion . 将啤酒酵母和毕赤氏酵母单倍体化、通过原生质体融合技术促使其融合。
- Objective To purify the hepatitis B virus(HBV) preS2(epi)S protein expressed in Pichia pastoris and study its immune effect. 目的研究重组毕赤酵母表达的乙型肝炎病毒preS2S蛋白的纯化方法及免疫效果。
