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- Keywords liquid chromatography/mass spectrometry;melanoma antigen encoding gene 2;epitope peptide;isomer;solvent;induced; 液相色谱/质谱联用;黑色素瘤抗原基因2;表位多肽;异构体;溶剂;诱导;
- Major protective antigen encoding r egion of E2gene of 2isolate strains o f HCV was amplified by RT-PCR techniq ues and sequenced. 用RT-PCR技术对华东地区2份猪瘟病料的E2基因主要抗原编码区域进行了扩增和序列分析。
- Complementary DNA (cDNA) sequence of Japanese sea bass (Lateolabrax japonicus) prion protein (PrP) encoding gene was cloned and characterized through RT-PCR approach. 采用RT-PCR方法分离了鲈鱼蛋白质感染因子蛋白编码基因序列,并进行了结构分析。
- It is possible to clone the encoding gene and identify its function to provide candidate loci for sprouting resistance breeding in wheat. 对控制该蛋白表达的基因进行克隆与功能鉴定可能为小麦抗穗发芽育种提供候选位点。
- The aim of this study was to construct antisense of ZNRD1 encoding gene, and to transfect it into SGC7901/VCR cells, to seek measures to overcome multidrug resistance of gastric cancer cells. 为获得ZNRD1基因的编码区序列 ,构建反义真核表达载体 ,通过检测ZNRD1基因反义核酸转染对胃癌长春新碱耐药细胞SGC790 1/VCR阿霉素 (ADM)积蓄能力的影响 ,评价ZNRD1在抗胃癌细胞多药耐药性方面的应用前景。
- Abstract: Nucleoside diphosphate kinase（NDPK） encoding genes are highly conservative in the evolution, but NDPKs reveal various biological funcions. 文章摘要: 核苷二磷酸激酶（NDPK）基因在进化中高度保守,却又呈现复杂多样的生物学功能。
- The encoding genes of IgG binding domains of staphyloccal protein A(SPA)nd streptococcal protein G (SPG)were cloned and expressed as fusion protein using pGEX vector of E. coli. 葡萄球菌A蛋白（SPA）和链球菌G蛋白（SPG）的IgG结合区编码基因融合后，克隆到大肠杆菌表达载体PGEX，SPA/SPG融合蛋白（SPAG）得到高效表达。
- Plasmids encoding genes for either green fluorescent protein or red fluorescent protein were bound to superparamagnetic nanoparticles coated with polyethyleneimine. 无论是编码绿色荧光蛋白还是红色荧光蛋白的质粒编码基因都是与外包聚乙烯亚胺的超顺磁性氧化铁颗粒结合。
- Recently,we had discovered and reported the first time the human G5 group A rotavirus strain LL36755 in Asia. To further explore the evolutionary origin,the VP4,VP6,NSP4 encoding genes of the strain LL36755 were amplified by RT-PCR. 最近在亚洲首次发现并报道了感染人的G5型人A组轮状病毒LL36755株,为进一步探讨其进化来源,克隆了G5型人A组轮状病毒LL36755株的VP4、VP6、NSP4编码基因,并分析其基因序列的分子特征。
- [Methods] To detect shiga toxin gene (stx), intimin gene (eaeA), hemolysin gene (hlyA) encoding gene of O157 antigen (rfb O157) and encoding gene of H7 antigen (fliC H7) by the method of polymerase chain reactions (PCR). [方法]应用聚合酶链反应(PCR),以志贺毒素基因(stx)、粘附抹平因子基因(eaeA)、溶血素基因(hlyA)、O157抗原编码基因(rfbO)157)和H7抗原编码基因(fliCH7)为靶基因进行检测。
- Sequence Analysis and Comparison of gp55 antigen encoding region of Hog Cholera Virus isolates of Changchun and Jilin 两个猪瘟病毒野毒株gp55基因抗原编码区序列的分析及比较
- Results:The new gene encoding the antigen epitope of SARS-CoV S protein was designed and synthesized s... 结论:可以重新设计新基因产生新型候选重组疫苗。
- The attraction between an antigen and an antibody. 化合力抗原和抗体之间的相互吸引
- Cloning and sequence analysis of the aspartokinase (AK) encoding gene 天冬氨酸激酶(AK)编码基因的克隆及其序列分析
- To fuse the gene encoding the surface antigen P1 with hspB of Coxiella burnetii and to express the p1 hspB fusion gene in E. coli cells. 目的 将贝氏柯克斯体 (Coxiellaburnetii)表面抗原P1蛋白基因与热休克蛋白B(HspB)基因片段融合 ,并使该融合基因在大肠杆菌细胞内表达 ,产生P1 HspB融合蛋白。
- Objective:To design a new gene encoding the antigen epitope of the spike protein(S protein) of severe acute respiratory syndrome coronavirus (SARS-CoV) and express the new gene. 目的:表达严重急性呼吸综合征(SARS)冠状病毒S蛋白的抗原表位序列,为研制新型的SARS病毒疫苗提供新思路。
- Each antigen may have many different determinants. 每一个抗原分子可以有数个不同的决定簇。
- Cloning and characterization of the prion protein encoding gene of Japanese sea bass, Lateolabrax japonicus 鲈鱼蛋白质感染因子蛋白基因的克隆和结构分析
- Norwalk virus antigen was detected by RT-PCR. 采用RT-PCR检测诺瓦克病毒抗原。
- The position that a given gene occupies on a chromosome. 基因座在一条染色体上某种给定基因所占的位置
