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- The adrenoreceptor density of peripheral lymphocyteswere measured by radio ligand binding assay,and the content of interleukin-5(IL-5) and interleukin-12(IL-12) were measured by enzyme-linked immunosorbnent assay. 用放射性配基结合分析法测定外周血淋巴细胞受体的变化,测定血清白介素5(interleukin-5,IL-5)、白介素12(interleukin-12,IL-12)含量。
- radioactive ligand binding assay 放射性配基结合分析
- receptor radio ligand binding assay 受体放射配体结合分析
- Keywords LHRH-PE40;radioactive ligand binding assay;affinity; 放射性配基结合分析;亲和力;
- Pressure Effects on Ligand Binding Kinetics for Hemoproteins. 压力对血红素蛋白配合基键联动力学的影响。
- Binding assay shown that Vp28 can not binding membrane protein of shrimp gill. 纯化的VP28与对虾鳃细胞膜的结合实验表明,VP28不能与对虾鳃细胞膜结合,即纯化的VP28不具有结合活性。
- The dopaminergic receptors were measured with the radio-ligand binding assay. 采用同位素标记法测定受体变化;
- Consequent upon the structural flexibility and the ligand binding, circulating albumin exhibits chemical and physical microheterogeneity. 由于其结构上的易变性和配体结合的不同,循环中的白蛋白表现出化学和物理上的微观不均一性。
- Some albumin molecules may hae undergone steric modification or ligand binding such that the immunoreactie sites hae become obscured. 某些白蛋白分子也许经历了空间修饰或配体结合上的改变以至于其免疫反应位点被湮没。
- Also the modeling presents the ligand binding site, active site and the putative binding of tRNA Trp . 模建结果对配基结合位点和活性位点以及可能与tRNATrp结合的方式也给出了预测。
- Competitive protein binding assay indicates the level of cAMP is improved when CDCA is given by oral administration. 通过竞争性蛋白质结合分析法测得,鹅去氧胆酸经灌胃给药后能显著提高大白鼠气管组织中cAMP的含量。
- We need to further validate the relationship between the newly discovered cholesterol sites and the ligand binding sites on these receptors. 我们需要进一步地确认在这些受体中新发现的胆固醇位点和配体位点的关系。”
- SSR was detected by radioligand binding assay(RBA) and ER, PR by LsAB method in these groups. 采用RBA法测定各组织的SSR表达 ,采用免疫LsAB法测定ER、PR表达。
- Sequence differences in the ligand binding domain of PXR serve as the molecular basis for the species difference in CYP3A induction. 化学物通过PXR调节CYP3A的表达可能是影响化学物体内代谢的一条重要途径。
- The effects of l-CSL on recombinant D_1R were investigated by [3~H]SCH23390 binding assay and cAMP assay. [~3H]SCH23390受体结合和cAMP测定实验检测左旋氯代斯阔任对重组D_1受体的激动作用。
- Method H?3?cAMP was used in protein binding assay to determine the level of cAMP in bovine BTC. 方法运用蛋白竞争结合法用氚(H3)标记的环磷酸腺苷(adenosine3,5?cyclicmonphos?phate,cAMP)测定牛眼小梁细胞内cAMP的含量。
- Methods: The number and affinity of IR on hepatocyte membrane of ASR were determined with radioligand binding assay. 采用放射配体结合分析方法检测肝细胞膜胰岛素受体的数目与亲和力。
- From the calculations, it can be found that the van der Waals interactions, the hydrophobic interactions, as well as the H-bonding interactions are crucial for ligand binding. 计算得到的抑制剂和靶酶之间的相互用模式显示范德华相互作用、疏水相互作用以及氢键作用是主要的作用模式。
- Methods Radioligand binding assay was used to determine the contents of GR and its affinity (Kd). 方法应用胃泌素受体的放射配基结合分析法,测定34例胃癌组织胃泌素受体的含量及其亲和力。
- Competitive inhibition assay, saturation binding assay and scatchard analysis testify the expression of LH/hCG receptor. 竞争结合实验、受体结合的特异性实验、饱和结合实验证明3AO细胞上存在特异性的Ll壬七CG受体。