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- The study of detection of 35S? NOS? Bt in 5 transgenic and 3 non-transgenic cotton seeds was carried out with DNA extraction by means of modified CTAB method and modified kit method respectively. 对5种转基因棉籽和3种非转基因棉籽,应用改良CTAB法和改良KIT法提取DNA模板,对35S、NOS、Bt等基因进行了检测研究,并确定了在检测Bt基因时的灵敏度。
- The CTAB-guanidinium method,modified CTAB method,and RNAplant Kit method were used to extract the total RNA from the plantlets of apple cultured in vitro,in which contain a lot of carbohydrates and phenolic compound. 利用CTAB_异硫氰酸胍法、改良CTAB法和RNAplant试剂盒法,从富含多糖和酚类物质的苹果组培苗继代苗中提取总RNA。
- Methods LDH and CK activities were continuously determined by commercial kit method using fresh specimen in COBAS MIRA plus analyzer. 方法LDH、CK测定均采用新鲜标本，成品试剂盒，用COBAS、MIRA、PLUS全自动生化分析仪连续监测法检测。
- The methods of isolating total RNA from plant tissues, including Improved CTAB, SDS-phenol, Hot borate, Guanidine Isothiocyanate and Trizol kit method, were compared and improved. 改良CTAB法和改进SDS-酚法可从田间叶片中得到高质量的RNA,改良CTAB法提取的RNA纯度高于SDS-酚法,但产率却显著低于SDS-酚法;
- The modified CTAB and RNAplant Kit methods were a bit short in total RNA extraction because of a little DNA contamination. And the RNAplant Kit method showed advantages of fastness and convenience. 改良CTAB法和RNAplant试剂盒法提取的RNA均有少量DNA污染,但RNAplant试剂盒法比CTAB法更快捷,简便。
- The results showed that protein K-SDS-phenol/chloroform and UNIQ-10 DNA Extraction Kit methods could obtain the highest total DNA yield and DNA purity as well as PCR analysis in impurity. 结果表明:蛋白酶K-SDS-酚氯仿法和柱提取DNA试剂盒法提取活性污泥总DNA效果最好,提取DNA总量多,纯度高,可不经纯化直接进行PCR扩增反应。
- The method seems good but it needs to be tried out. 这个方法似乎不错，但需要试验一下。
- The nurse has taken a first-aid kit. 护士已经带上了一套急救用品。
- Kit this man out with everything he needs. 他需要什麽就给他什麽装备。
- The soldiers shouldered their kit and moved on. 士兵们背起行装，继续前进。
- This toy ship is made from a kit. 这艘玩具船是由配套元件装配而成。
- Always keep a first-aid kit handy. 手边要经常有个急救箱。
- They marched twenty miles in full kit. 他们带着全副装备行军二十英里。
- I can't claim infallibility for this method. 我不敢说这方法绝对有效。
- Summer fallow is the best method of destroying weeds. 夏季休耕是消灭杂草的最好办法。
- He seized on his kit bag and ran out with his comrades. 他抓起工具袋和同志们一起奔了出去。
- Popularizing this new method is not an easy job. 推广这种新方法不是件容易的工作。
- This is not an economical method of heating. 这不是一种经济的取暖方式。
- I have a simple and easy method. 我有个简易的方法。
- I went through by a summary method. 我成功运用了一个简易的方法。
