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- Research Advancements of Genomic Infectious cDNA Clone of Positive-Strand RNA Virus 正链RNA病毒基因组感染性克隆研究进展
- Sequence Analysis and Construction of Infectious cDNA Clone of Beet Black Scorch Virus Xinjiang Isolate 甜菜黑色焦枯病毒新疆分离物基因组RNA的序列分析及侵染性cDNA克隆构建
- Construction of infectious cDNA clone of Cucumber mosaic virus satellite RNA XJs1 and preliminary study on its biological function 1侵染性cDNA克隆构建及其生物学功能初步研究
- infectious cDNA clone 侵染性cDNA克隆
- Objective To construct the cDNA subclones spanning the entire genome of dengue 2 virus NGC strain for further construction of full-length infectious viral cDNA clone. 目的构建登革2型病毒NGC株基因组全序列的亚cDNA克隆,为进一步构建全长感染性cDNA克隆奠定基础。
- The TuMV isolate CDN1 infectious full-length cDNA clone pVIR95T was modified into an expression vector pVIR95TM by inserting the adaptor with the MCS and the new NIa recognition site after the site between the Nib and CP by PCR. 在此基础上，将含有多克隆位点和编码NIa蛋白酶识别位点的Adaptor通过PCR介导插入到芜菁花叶病毒CDN1株系全长cDNA的NIb与CP区之间的NIa蛋白酶识别位点之后，将芜菁花叶病毒CDN1株系全长cDNA侵染性克隆pVIR95T改造成一个可利用的基因插入型表达载体pVIR95TM。
- infectious full-length cDNA clone 感染性全长cDNA克隆
- We introduced the cDNA clone of ARV-2 from USA in 1988. We subcloned this full-length cDNA into pUC19 by Kpn I site. 艾滋病相关病毒(ARV-2)是目前应用最广的艾滋病毒分离株之一.
- A cDNA clone of 1 629 bp was obtained by screening the library with an adult stage specific probe. 利用消减杂交 (subtractedhybridization)获得的旋毛虫成虫期特异性探针筛选成虫cDNA文库 ,获得一个长 16 2 9bp的基因。
- A cDNA clone encoding a pheromone binding protein 3 from antenna of Helicoverpa assulta (named HassPBP3) was isolated by reverse transcription polymerase chain reaction (RT-PCR). 利用RT-PCR技术从烟实夜蛾Helicoverpa assulta( Hass)雄虫触角中扩增得到了信息素结合蛋白3( HassPBP3)。
- The full-length cDNA clone, NDV3GM122, and the three helperplasmids pCI-NP, pCI-P and pCI-L of Newcastle disease virus strain ZJI isolated from an outbreak in the goose were cotransfected into BSR-T7/5 cell expressing T7 RNA polymerase. 利用反向遗传操作技术,将ZJI株鹅源新城疫病毒全基因组cNDA克隆(NDV3GM122)和含该毒株NP、P及L基因的3个表达载体(pCI-NP、pCI-P与pCI-L)共转染BSR-T7/5细胞;
- Construction of Infectious Chimeric cDNA Clone of Sindbis Virus by Triple Fusion PCR 三重融合PCR法构建感染性辛德毕斯嵌合病毒cDNA克隆
- full-length genomic cDNA clone of Gx strain Gx株全长cDNA
- CDNA cloning and sequence analysis of coat protein genes of TMV and CMV isolated from tobacco in Yunnan province. 云南省烟草花叶病毒和黄瓜花叶病毒外壳蛋白基因克隆及序列分析。
- Methods:APAF-1 cDNA types in leukemic cell lines K562 and CEM/VLB100 were studied by cDNA cloning strategy. 方法:应用RT-PCR结合测序方法研究白血病细胞系K562和CEM/VLB100APAF-1cDNA类型;
- Forty-four cDNA clones, representing 44 defense-responsive genes, were fine mapped to 56 loci distributed on 9 of the 12 rice chromosomes. 44个cDNA克隆代表44个抗病相关基因被定位于水稻12条染色体中的9条染色体上的56个位点。
- Betaine aldehyde dehydrogenase (BADH) cDNA cloned from Atriplex hortensis L. was transferred into tetraploid clone of black locust (Robinia pseudoacacia L.) by agrobacterium (Agrobacterium tumefaciens Conn) mediated transformation. 利用农杆菌(Agrobacterium tumefaciens Conn)介导法;将山菠菜甜菜碱醛脱氢酶(BADH)基因转入四倍体刺槐(Robinia pseudoacacia L.;)
- The availability of cDNA clones for EcR and USP from several insect species has opened up possibilities for the development of high throughput EcR/USP based screening assays. 多种昆虫蜕皮激素受体和超气门蛋白基因的成功克隆为建立基于昆虫蜕皮激素受体的高通量筛选技术奠定了基础，并且可以通过多种途径实现。
- He expressed infectious enthusiasm to his voters. 他对他的选民表现出很有感染力的热情。
- The cDNA Cloning of Pokeweed Antiviral Protein Gene and Research on Gene-transforming Tobacco[J].Journal of Henan Agricultural University,2006,40(2):130-132,141. PAP基因cDNA克隆及烟草转基因研究[J].;河南农业大学学报;2006;40(2):130-132;141
