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- indirect fluorescence assay 间接荧光测定
- mAbs against VP22 were prepared by hybridoma technology from BALB/c mice immunized with the fusion protein GST-VP22C and characterized by ELISA,indirect fluorescence assay(IFA) and Western blot. 将该表达产物切胶免疫小鼠,利用杂交瘤技术制备抗MDV-1 VP22C的mAb,并通过ELISA、间接免疫荧光(IFA)、Western blot鉴定其特性。
- Indirect immuno fluorescence assay (IFA) showed that the chimeric antibody has similar capacity of binding Chik virus antigen as mouse McAb. 用间接免疫荧光实验(indirect immunofloresence assay,IFA)检测显示,纯化的嵌合抗体与Chik病毒抗原发生强反应,说明表达的嵌合抗体保留了亲本鼠源McAb的抗原结合活性。
- Reticuloendotheliosis Virus (REV);Transfection;Genome;Polymerase Chain Reaction(PCR);env gene;Homology;Fusion protein;Indirect Fluorescent Assay(IFA 01禽网状内皮组织增生症病毒;转染;基因组;囊膜糖蛋白基因;同源性;表达;间接免疫荧光
- The use of enzymes as labels reflects the move away from radioisotopes and one of the most powerful non-radioisotope procedures is the time-resolved fluorescence assay. 酵的用途标签从放射性同位素那里到远处反映出行动并且最强有力的非放射性同位素的程式之一是时间解决的莹光分析。
- enzyme-linked fluorescence assay 酶联荧光测定
- time- resolved fluorescence assay 时间分辨荧光分析
- An indirect fluorescent antibody technique(IFAT) has been developed to detect Hematodinium sp. 应用该抗体建立了血卵涡鞭虫的间接荧光抗体检测技术(IFAT)。
- Toxoplasma direct agglutination test (DAT) was evaluated by comparing it with the conventional indirect fluorescent antibody test (IFA). 为了评价新建立的弓形虫直接凝集试验(DAT),对DAT和常规的间接荧光抗体试验(IFA)作了比较研究。
- Specific bright yellow green flurescene was observed in the plasmas of infected cell by indirect fluorescent antibody test. 用间接荧光抗体试验在细胞浆中观察到特异性黄绿色荧光;
- Methods Surveying epidemiologically the area with the indirect fluorescent antibody test(IFAT)and checking the parasite rate again in 1999. 方法于1999年再次应用IFAT进行流行病学监测及原虫率考核。
- Keywords fluorescence assay;rosaniline(RL);bovine serum albumin; 荧光分析法;碱性品红(RL);牛血清白蛋白;
- Methods Indirect fluorescent staining, FCM analysis and RT PCR were employed to detect the expression and regulation of PTA1 at the levels of PTA1 protein and PTA1mRNA. 用RT PCR方法检测内皮细胞中PTA1mRNA的表达 ;
- Keywords Fluorescence assay;rosaniline;deoxyribonucleic acid; 荧光分析法;碱性品红;脱氧核糖核酸;
- Wang SP, Grayston JT. Serotyping of Chlamydial trachomatis by indirect fluorescent - antibody staining of inclusions in cell culture with monoclonal antibodies [J]. J Clin Microbiology, 1991,29:1295. 余加林;吴仕孝.;连接酶链反应在沙眼衣原体感染诊断中的应用[J]
- The equipment shall be composed of: microplate reader for ELISA( or analyser ELISA, analyser enzyme fluorescent assay), microplate washer, micropipette, water-bath or temperature cabinet, refrigerator, shaker for microplate. Etc. 仪器设备组合应为:酶标仪(酶免分析系统、免荧光分析仪等)洗板机、样器、浴箱或温箱、箱、标板振荡器等。
- We will make an assay of the ore that he brought. 我们将对他带来的矿石做实验鉴定。
- His indirect way of telling me to leave annoyed me. 他那种不直截了当地让我离开的方式激怒了我。
- Determination of Diclofenac Sodium by Indirect Fluorescence Spectrophotometry 间接荧光猝灭法测定双氯芬酸钠
- Determination of Diclofenac Sodium bv Indirect Fluorescence SoectroDhotometrv 间接荧光猝灭法测定双氯芬酸钠