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- It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
- RESULTS: Most of the product didn t form inclusion body in cell. 结果:表达产物在细胞内大部分没有形成包含体。
- The virus replicated in the intracytoplasm and formed inclusion bodies. 病毒在细胞浆内复制，可形成特征性包涵体；
- The chicken inclusion body hepatitis virus could induce SPF chicken to synthesize TNF with the infection by mouth. 研究结果表明,鸡包涵体肝炎病毒经口腔感染SPF雏鸡可刺激机体产生TNF,在感染的不同日龄鸡的TNF活性出现一定的消长规律。
- Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表达产物为包涵体形式，溶解和折叠后，以GST亲合层析色谱纯化。
- With anti-CDV antigen assay, the ependymal cells with inclusion bodies showed an intensive positive reaction. 部分锥体细胞，神经核细胞和浦氏细胞变性、溶解或胞浆深染，胞核浓缩。
- It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. 它也包括关于包涵体，熔化蛋白质和结晶学的洗净的洗净的章。
- The monolayer BEF with CPE was stained by HE, and the inclusion bodies were found in the enlarged cell nuclei. CPE表现为细胞肿大、变圆、脱落，HE染色可见到病变细胞内有核内包涵体。
- The methylene blue-fuchsin basic stain is instable, being related with character of the inclusion bodies. 但采用亚甲蓝-碱性品红法显示病毒包涵体的效果不稳定，可能与包涵体嗜性有关。
- Intracytoplasmic inclusion bodies, the socalled molluscum bodies, are seen in the upper epidermis. 传染性软疣 表皮上部见胞质内包涵体，即软疣小体 Molluscum contagiosum.
- After isolation, solubilization and refolding, inclusion bodies turn to active proteins in vitro. 包涵体在体外经分离、溶解与重折叠后可实现复性，表现为具有生物活性的蛋白。
- Inactive inclusion bodies are often formed during overexpression of recombinant proteins in prokaryotes. 用基因工程技术在大肠杆菌高水平表达重组蛋白时，通常形成无生物活性的包涵体。
- Objective To investigate the clinical and pathological features of inclusion body myositis (IBM). 摘要目的探讨包涵体肌炎的临床与病理特点。
- Methods: Wash inclusion body effectivty, use Hydrophobic Interaction Chromatography to carry on the purification. 方法：对包涵体进行有效洗涤，采用疏水层析进行纯化。
- The strains with recombinant plasmid of hEC-SOD were induced and expressed as inclusion body. 对含人EC-SOD重组质粒的工程菌进行诱导表达形成包涵体。
- The virions and its inclusion bodies were observed in both nucleus and cyto plasm. 在细胞核及细胞浆内均形成圆形或椭圆形的病毒包涵体。
- Then the inclusion bodies were redissolved by 7 mol/L guanidine hydrochloride containing 10 mmol/L of DTT. 而稀释复性法是研究其它复性方法的基础，试验中先用含2 mol/L 尿素的0.;1 mol/L Tris-HCl 缓冲液对包涵体进行洗涤，然后用含10 mmol/L DTT 的7 mol/L盐酸胍溶解包涵体。
- The 10.6 kDa His-hepcidin expressed from pET-hpc was exitsted in the form of insoluble inclusion bodies. 本文采用基因工程菌表达的His-hepcidin融合蛋白包涵体，通过IMAC、氧化、复性及酶切，得到了具有生物活性的hepcidin重组蛋白，并验证了重组蛋白的抗菌活性。
- Objective:To investigate the clinical and pathological features of inclusion body myositis(IBM). 目的探讨包涵体肌炎(IBM)的临床表现与病理特点。
- Refolding of protein from inclusion bodies were first denatured by using high dpnaturant and detergent. 在包涵体蛋白复性过程中,溶解的蛋白质容易相互聚集生成沉淀,成为复性技术中最难以克服的问题。
