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- The virus replicated in the intracytoplasm and formed inclusion bodies. 病毒在细胞浆内复制,可形成特征性包涵体;
- Expressed proteins as inclusion bodies were solubilized, refolded and purified by GST affinity chromatography. 表达产物为包涵体形式,溶解和折叠后,以GST亲合层析色谱纯化。
- With anti-CDV antigen assay, the ependymal cells with inclusion bodies showed an intensive positive reaction. 部分锥体细胞,神经核细胞和浦氏细胞变性、溶解或胞浆深染,胞核浓缩。
- It also includes chapters on purification of inclusion bodies, fusion proteins, and purification for crystallography. 它也包括关于包涵体,熔化蛋白质和结晶学的洗净的洗净的章。
- The monolayer BEF with CPE was stained by HE, and the inclusion bodies were found in the enlarged cell nuclei. CPE表现为细胞肿大、变圆、脱落,HE染色可见到病变细胞内有核内包涵体。
- The methylene blue-fuchsin basic stain is instable, being related with character of the inclusion bodies. 但采用亚甲蓝-碱性品红法显示病毒包涵体的效果不稳定,可能与包涵体嗜性有关。
- Intracytoplasmic inclusion bodies, the socalled molluscum bodies, are seen in the upper epidermis. 传染性软疣 表皮上部见胞质内包涵体,即软疣小体 Molluscum contagiosum.
- It turns that most of the recombinant protein were inclusion body. 对重组蛋白的细胞内定位分析发现蛋白几乎全部以包涵体形式存在。
- RESULTS: Most of the product didn t form inclusion body in cell. 结果:表达产物在细胞内大部分没有形成包含体。
- After isolation, solubilization and refolding, inclusion bodies turn to active proteins in vitro. 包涵体在体外经分离、溶解与重折叠后可实现复性,表现为具有生物活性的蛋白。
- Inactive inclusion bodies are often formed during overexpression of recombinant proteins in prokaryotes. 用基因工程技术在大肠杆菌高水平表达重组蛋白时,通常形成无生物活性的包涵体。
- The virions and its inclusion bodies were observed in both nucleus and cyto plasm. 在细胞核及细胞浆内均形成圆形或椭圆形的病毒包涵体。
- Then the inclusion bodies were redissolved by 7 mol/L guanidine hydrochloride containing 10 mmol/L of DTT. 而稀释复性法是研究其它复性方法的基础,试验中先用含2 mol/L 尿素的0.;1 mol/L Tris-HCl 缓冲液对包涵体进行洗涤,然后用含10 mmol/L DTT 的7 mol/L盐酸胍溶解包涵体。
- The 10.6 kDa His-hepcidin expressed from pET-hpc was exitsted in the form of insoluble inclusion bodies. 本文采用基因工程菌表达的His-hepcidin融合蛋白包涵体,通过IMAC、氧化、复性及酶切,得到了具有生物活性的hepcidin重组蛋白,并验证了重组蛋白的抗菌活性。
- Refolding of protein from inclusion bodies were first denatured by using high dpnaturant and detergent. 在包涵体蛋白复性过程中,溶解的蛋白质容易相互聚集生成沉淀,成为复性技术中最难以克服的问题。
- The active anti-AIF scFv was recovered efficiently from inclusion bodies with a refolding yield of approximate 75% confirmed by spectrophotometer. AIF4c9scFv表达蛋白在细胞质中主要以不溶性包涵体形式存在,优化后的柱内复性程序可从高浓度的包涵体变性蛋白中高效复性AIF4c9scFv表达蛋白,其回收率可达75%25左右。
- The secretion of recombinant protein to periplasm or extracellular medium has more advantages than the expression intracellular as inclusion bodies. 重组蛋白分泌到周质空间或胞外培养基中较之在胞内以包含体形式表达有许多优势。
- The inclusion bodies located in ependymal cells were a important evidence for determination of canine distemper in brain tissues. 位于室管膜细胞内的包涵体对于脑组织犬瘟热的确诊具有重要的作用;
- IHHNV typically infects the tissues of ectodermal and mesodermal origin, showing eosinophilic intranuclear inclusion bodies. 肉眼检查,感染白点症、传染性皮下及造血组织坏死及套拉症之白虾种虾,皆无肉眼病变。
- Acidophilic round or oval viral inclusion bodies were found in the cyst wall cells and keratinized meshes of cystic cavity. 皮损组织病理检查显示真皮内见一囊肿,囊壁细胞内见嗜酸性圆形或椭圆形病毒包涵体,囊腔内的角质物网眼内亦见病毒包涵体。