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- The major bond of conjugation in the formation of complex was probably in the hydrogen bonding and the hydrophobic interaction. 在复合物形成中主要的结合键可能是氢键,以及疏水性作用力。
- Methods: Wash inclusion body effectivty, use Hydrophobic Interaction Chromatography to carry on the purification. 方法:对包涵体进行有效洗涤,采用疏水层析进行纯化。
- It was found that the adsorption of proanthocyanidins on DTA, ADS-8 and ADS-17 was driven by both hydrophobic interaction and hydrogen bonding. 结果表明,DTA、ADS-8、ADS-17对原花青素的吸附既包含疏水作用又有氢键参与。
- The retention behaviors of aromatic alcohol homologues in hydrophobic interaction chromatography (HIC) were investigated firstly. 首次研究了芳香醇同系物在疏水色谱(HIC)中的保留行为。
- The retention mechanism of dencichine on a porous graphitic carbon column seems to be mainly hydrophobic interaction according to the study. 对三七素在石墨化碳柱上的保留及其影响因素的研究结果表明:三七素在石墨化碳柱上的保留机理仍主要是疏水相互作用。
- IAMC, compared with n-octanol/water, liposome/water and ODS chromatography, can simulate other forces besides hydrophobic interaction. 目前考察药物跨膜转运的模型主要有正辛醇/水系统、脂质体/水系统、反相色谱(ODS)以及磷脂膜色谱。
- Amphiphilic polymer can self-assemble into supermolecular structure(polymer micelle)by the balance of the hydrophilic and the hydrophobic interaction. 双亲聚合物在选择性溶剂中通过亲水作用与疏水作用间力的平衡,可自组装形成核-壳型超分子有序结构(聚合物胶束);
- Then the refolding of protein in the ion-exchange chromatography (IEC), hydrophobic interaction chromatography (HIC) and size-exclusion chromatography (SEC) was studied. 所以本文将首先从描述液-固界面的模型入手,研究溶质在液-固界面的吸附行为受到的影响,在此基础上对变性蛋白在离子交换色谱(IEC),疏水色谱(HIC)及排阻色谱(SEC)中的复性进行研究。
- Cecropin-XJ expressed in Pichia pastoris was purified through ion exchange chromatobraphy(IEC),hydrophobic interaction chromatography(HIC) and size exclusion chromatography(SEC). 通过离子交换层析、疏水作用层析和分子排阻层析等三步层析的方法,分离纯化了毕赤酵母工程菌所表达的新疆家蚕抗菌肽。
- FSA is an assembly process using fluid to transport components to binding sites and the components can orient itself on the binding sites by hydrophobic interaction. 由于流体自我组装元件利用疏水性与亲水性之表面性质差异,驱使具疏水性表面之元件组装于基板上方特定之位置。
- Influences of concentration of water in the mobile phase on separation were studied,and the results showed that hydrophobic interaction was the major interaction force in mol... 通过对流动相中水含量与色谱分离效果关系的探讨,结果表明溶质与印迹固定相的疏水作用是分子识别中的主要作用力。
- Based on the property of bacterial endotoxin,the effects of affinity chromatography,ion exchange chromatography and hydrophobic interaction chromatography on removal of endotoxin in the pu- rification of recombinant12 5Ser- IL - 2 were compared. 针对细菌内毒素的化学性质 ,比较了用亲和层析法、离子交换层析法和疏水相互作用层析法等 3种层析法去除基因重组 1 2 5Ser- IL- 2产品中细菌内毒素的效果。
- Adsorpted Calmodulin could not be eluted by EQTA or high salt solution and it was eluted by 50% ethanol. This results indi cate that the hydrophobic binding to the Polymer is independent of calcium induced hydrophobic interaction on Calmodulin. 钙调蛋白在3520吸附树脂上的选择性吸附作用不依赖于钙离子的存在,EGTA或高浓度的盐溶液均不能洗脱,但可被50%25乙醇洗脱,此结果表明钙调蛋白与吸附树脂的结合为疏水结合,与钙离子诱导的疏水区变化无关。
- Influences of concentration of water in the mobile phase on separation were studied, and the results showed that hydrophobic interaction was the major interaction force in molecul... 通过对流动相组成与色谱分离效果关系的探讨,证实溶质与印迹固定相的疏水相互作用是分子识别的主要作用力。
- Hydrophobic interaction chromatography (HIC) is widely used in protein separation and purification.Butyl-agarose,phenyl-agarose and octyl-agarose are the most important HIC media. 疏水层析是广泛应用于生物大分子分离纯化的技术,丁基琼脂糖(butyl-agarose)层析介质、苯基琼脂糖(Phenyl-agarose)层析介质、辛基琼脂糖(octyl -agarose)层析介质是常用的疏水分离介质。
- The binding of ligands to proteins occurs mostly through weak non-covalent interactions, namely hydrophobic interaction, van der Waals forces, and hydrogen bonding. 蛋白和配体间的作用通常是通过弱非共价键力的相互作用而实现的,如疏水作用、范德华力和氢键。
- Hydrophobic interactions and hydrogen bonds played the main function, and disulfide bonds was the second during peanut protein texturization. 在花生蛋白高水分挤压组织化过程中,疏水作用和氢键起主要作用,其次是二硫键。
- From the calculations, it can be found that the van der Waals interactions, the hydrophobic interactions, as well as the H-bonding interactions are crucial for ligand binding. 计算得到的抑制剂和靶酶之间的相互用模式显示范德华相互作用、疏水相互作用以及氢键作用是主要的作用模式。
- These experimental results showed that hydrogen bonds、electrostatic forces and hydrophobic interactions have given importhant contribution for soybean protein to form tran... 这为进一步研究大豆蛋白凝胶的光学性质和研制透明的大豆蛋白产品提供理论依据。
- hydrophobic interaction chromatography 疏水作用色谱法