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- Conclusion The hybridoma cell line may be applicable to the diagnosis of HBsAg. 结论筛选出了一株适合临床应用的单抗杂交瘤细胞株。
- McAb hybridoma cells then were injected into other mice abdominal cavity to prepare ascites again. 再将此分离出的杂交瘤细胞，注入其他小鼠腹腔又制得腹水。
- Objective To found a hybridoma cell cloning method when we established strains of hybridoma cells to F1 antigen. 目的探讨在建立了分泌抗鼠疫F1抗原的单克隆抗体杂交瘤株后,对其进行克隆化的实验方法。
- The hybridoma cell strain E3E6 secreting monoclonal antibodies was screened and clo ned by indirect ELISA. 通过用间接ELISA试验筛选、克隆出分泌抗RHDV单克隆抗体的杂交瘤细胞林E_3E_6。
- Results: Hybridoma cells growed well in 12 spots with DMEM culture.And of the 12 spots,6 spots chromosomes had good shapes and stead numbers. 结果:12孔板中加入DMEM培养液的杂交瘤细胞株生长良好,其中6孔板加秋水仙素培养1h,染色体形态最佳,数目稳定。
- Results A hybridoma cell line secreting McAb steadily with high titer was obtained, which was named SU-2000. 结果 得到1株稳定分泌抗体、效价高的杂交瘤细胞株，命名为SU-2000。
- Yuanxing Zhang, Wangfun Fong, Pingpei Yung (1998) Optimization in hybridoma cell culture. 张立，沈红，张元兴*(2000)在动态和静态培养条件下杂交瘤细胞的生长和代谢。
- Results of controlled experiments with hybridoma cells indicated that theretention efficiency of viable cells was higher th... 所建过程模型对过程操作具有预见性,可用于过程参数的估计,并可指导沉降装置的设备放大。
- The hybridoma cell lines could produce the McAbs which was specific to BDV P24 and P40 antigens with the appropriate titers. 杂交瘤细胞系H1和H2可以产生一定效价的单克隆抗体,并对BDV的P24和P40抗原具有特异性。
- The monoclone hybridoma cell line obtained was named SRBC-C, which secreted anti-human B grouping red cell antibody and was used for ABO blood typing. 该株能分泌抗B抗体,与B型红细胞发生凝集,对鉴定ABO血型有一定价值。
- The effects of glucose, L-glutamine, ammonia and lactic acid on the growth of mouse-mouse hybridoma cell line G_5F_4 were studied. 研究了葡萄糖、谷氨酰胺、氨和乳酸对鼠鼠杂交瘤细胞G_5F_4生长的影响。
- The results showed that compared with myeloma cell, marked changes were not observed in hybridoma cell in cell energy and cell appearance. 结果表明，与凯骨髓瘤细胞相比，杂交瘤细胞的细胞活力和细胞形态无明显变化；
- In the incubator, JAL1 hybridoma cells were cultivated in flask bottles in batch mode and the growth and metabolism of JAL1 hybridoma cells were studied. 研究了不同葡萄糖、谷氨酰胺浓度下JAL1细胞的生长、糖耗、乳酸及氨的生成、单抗合成等代谢过程及该株细胞的基本代谢规律,同时对该株细胞生长和单抗生产的动力学方面进行了研究。
- The hybridoma cell line with the collection number of CGMCCNO.1908 is used for a secrete anti-URG11 monoclonal antibody hybridoma cell line. 所述的保藏号为CGMCCNO.;1908的杂交瘤细胞株用于分泌抗URG11单抗的杂交瘤细胞株。
- Methods:The mice spleen cells were fused with SP2/0 cells to prepare the hybridoma cell in different culture medium.The acting time was changed when chromosome was prepared. 方法:取小鼠脾细胞与骨髓瘤细胞SP2/0常规融合,用不同的培养基制备杂交瘤细胞,并在制备杂交瘤染色体时改变秋水仙素作用的时间。
- The chromosome number of post-fused hybridoma cell was near to the cumulative chromosome number of splenic cell and myeloma cell. 当脾细胞与凯骨髓瘤细胞融合形成杂交瘤细胞后，其染色体数目接近两亲本细胞染色体数目之和。
- Objective: To study the effects of bovine retinal extract (BRE) on production of human IgM (hIgM) in the culture of human-mouse hybridoma cells named G12 . 目的：探讨牛视网膜提取物对人-鼠杂合细胞抗体产生的影响。
- RESULTS: Four hybridoma cell lines targeting a suitable peptide from pf HRP-II were obtaind by using spleen embedment method and hybridoma technology. 结果： 应用脾脏埋植法和杂交瘤技术， 获得4 株针对恶性疟原虫HRP-II单一表位的杂交瘤细胞。
- In 1. 5 L Celligen bioreactor, when pluronic F-68 concentration is 0. 10%(W/V) in medium and agation speed is 70r/min the hybridoma cell can grow normally. 在1.;5升GelliGen生物反应器中;培养基添加0
- Conclusion Plasmodial aldolase has been successfully expressed and purified， and the established hybridoma cell lines can secrete McAbs specific to the aldolase of P. falciparum. 结论 本实验构建并表达了重组疟原虫糖酵解醛缩酶，并获得特异性的单克隆抗体。
