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- We must prove that there is really brain glioma antibody in the blood of patients, then we can establish the base for constructing humanized phage antibody library. 为了构建人源性抗脑胶质瘤抗体库,我们必须先从患者的血液内检测到抗脑胶质瘤抗体,才能为建立人源性噬菌体抗体库奠定基础。
- DNA sequence analysis of heavy chain of human phage antibody to HBsAg 抗HBsAg人源噬菌体抗体重链基因的序列分析
- Construction of a human phage antibody library against mumps viruses 人源性抗腮腺炎病毒噬菌体抗体库的构建
- Construction and screening of the human phage antibody library from a patient with melanoma 黑素瘤患者噬菌体抗体库的构建与筛选
- Selecting, sequencing and expressing of the human phage antibody fragments against HTV 人源性汉坦病毒噬菌体抗体基因的筛选、测序和表达
- human phage antibody 人源噬菌体抗体
- The murine phage antibody library was amplified and then panned by human chorionic gonadotropin for four rounds.The last round enriched phage clones were used to reinfect. 由鼠源噬菌体抗体库淘选到展示有人绒毛膜促性腺激素单链抗体的噬菌体克隆。
- Objective: To construct human tetanus immune phage antibody library, screen antibody genes against tetanus toxoid, and express solution Fab in Escherichia coli. 目的:建立人破伤风免疫噬菌体抗体库,筛选抗破伤风类毒素的抗体基因,并在大肠杆菌中表达可溶性Fab段。
- Methods By genetic engineering technology,human anti-keratin ScFv was constructed from Fab fragment selected from established semi-synthetic phage antibody library. 方法利用基因重组技术对从半合成噬菌体抗体库中克隆的人源性抗角蛋白抗体Fab片段进行改造,获得了人源性抗角蛋白ScFv片段,并进行DNA序列分析。
- Conclusion Human anti-keratin scFv is obtained from a large phage antibody library, which will benefit to the application of anti-keratin antibody to clinical research. 结论利用噬菌体抗体库技术获得了人源性抗角蛋白单链抗体,为临床应用研究提供了具有更广阔应用前景的人源小分子抗体。
- Our previous studies have screened a clone of human anti-keratin antibody (FK315) from a semisynthetic phage antibody library, and successfully obtained its soluble expressing Fab fragments. 有鉴于此,我们己自半合成噬菌体抗体库中筛选、克隆了一株人源性抗角蛋白抗体FK3,并成功得到可溶性表达的 Fab段。
- Hie resultant vector was checked by expression of phage antibody, ELBA and in vivo recombination. 通过噬菌体抗体的表达、ELISA及细胞内重组试验鉴定所获得的表达载体。
- AIM: To construct a anti dodecane tertraacetic acid yttrium (DOTA Y) immune Fab phage antibody library. 目的 :构建钇 十二烷四乙酸 (Y DOTA)免疫噬菌体Fab抗体库。
- Abstract:Objective: To clone human anti-keratin antibodies from semisynthetic phage antibody library. 摘 要:目的: 从半合成噬菌体抗体库中筛选人源性抗角蛋白抗体并进行鉴定。
- Objective To select anti VEGF (vascular endothelial growth factor) antibodies (Fab fragments) from human phage display antibody library and to identify their specification and activities. 目的 从人噬菌体Fab抗体库中筛选抗人血管内皮细胞生长因子 (VEGF)抗体克隆 ,并对其特异性及活性进行分析 ,为后续工作奠定基础。
- AIM: To construct the phage antibody library for Fab fragment from a convalescent patient infected with SARS coronavirus. 目的 :利用抗SARS冠状病毒IgG抗体阳性的SARS康复患者外周血淋巴细胞 ,构建人源Fab段抗体文库。
- Phage antibody chips, prepared by coupling the recombinant phage displaying scFv with carboxyl-terminated silicon, can be applied in detecting proteins and proteomes. 摘要将展示单链抗体的重组噬菌体与羧基终止的矽片偶联,制成噬菌体抗体芯片,可用于检测多类蛋白质和蛋白质组。
- The phage antibody library technology is a novel method with many potential applications, consisting of antibody library and phage surface display technology. 噬菌体抗体库技术是抗体基因文库技术和噬菌体表面展示技术相结合形成的一项新技术与方法,在生物科学领域极具潜力。
- Wang Y, Hua B, Liu QY, et al.By-passing immunization: Construction of a phage antibody library with randomized CD3[J].ChinJ Microbiol Immunol, 1997, 17(6): 449-52. [5]王琰;化冰;刘群英;等.;随机化CD3抗体库的构建及不经免疫制备抗体的初步探讨[J]
- human natural phage antibody library 人源天然噬菌体抗体库