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- A characteristic cytopathic effect of CMV presented in a culture of human embryonic fibroblasts with conjunctival swab. 这些有病变反应之细胞经免疫萤光染色检定,对巨细胞病毒单株抗体染色呈阳性反应。
- The separation of primary mouse embryonic fibroblast (PMEF) and human embryonic fibroblast (HEF). 小鼠胚胎成纤维细胞(PMEF)和人胚胎成纤维细胞(HEF)的分离培养
- Feeder cells with murine embryonic fibroblasts(MEF) were better than that with human embryonic fibroblasts(hEF), or STO or bovine embryonic fibroblasts (bEF). MEF、STO、bEF和hEF可提高人原始生殖细胞(PGCs)的贴壁率,能促进PGCs源的人类胚胎干细胞(ES)的分离效率; 以MEF作为饲养层效果较好。
- Methods Neomycin gene was transfected into human embryonic fibroblasts (HEF) by liposome mediated method. Using this transfected HEF as feeder layer, ES cells were transfected with AANAT gene. 方法 将外源性neo基因转染人胚胎成纤维细胞 ,作为饲养层细胞用于ES细胞转基因后筛选及增殖过程的培养。
- Adaptive response induced by low concentration of hydroquinone in human embryonic fibroblasts cells 低剂量氢醌诱导人胚肺成纤维细胞适应性反应的研究
- human embryonic fibroblasts 人胚胎成纤维细胞
- human embryonic fibroblast 人胚胎成纤维细胞
- Objective To study the characteristics of Z-HL16C cell strain mutated from the human embryonic lung fibroblasts (HL). 摘要目的初步鉴定人胚肺成纤维细胞(HL)突变株Z-HL16C的转化性质。
- Methods The expression of IL-6 at transcriptional level was det ected in human embryonic lung fibroblasts by means of cell culture and RT-PCR. 方法 采用细胞培养与逆转录聚合酶链反应 (RT PCR)技术 ,研究IL 6在转录水平的表达。
- Objective: To observe the influence of Yujinfang capusle on electron microscope observation of Human embryonic lung fibroblast. 摘要目的:观察人胚肺成纤维细胞细胞膜表面与微细结构和含玉金方的血清的影响。
- Zhou JX, Liu D, Zheng KL, Deng CH, Wen RQ, Tang LX, Ma CJ.Culture conditions of mouse embryonic fibroblasts and preparation of feeder layer. 关键词:胚胎成纤维细胞;
- Human embryonic kidney(HEK)293T cells were transfected with these plasmids. 证实序列正确后,将上述质粒分别转染人肾胚胎上皮细胞(HEK)293T。
- Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast(MEF) cells for isolation and culturing of embryonic stem(ES) cells. 目的:建立ICR小鼠胚胎成纤维细胞饲养层培养体系,用于胚胎干细胞建系及研究。
- Objective:To approach advance of researching the human embryonic stem cell. 目的探讨人类胚胎干细胞的研究进展和应用前景。
- Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization. 方法取大鼠胚胎分离成纤维细胞,分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
- Human embryonic lung diploid fibroblasts 人胚肺成纤维细胞
- EPA significantly stimulated the growth of human embryonal pulmonary fibroblast (HPF) dose-dependently(P< 0.01). EPA对人胚肺成纤维细胞(HPF)生长起促进作用;且呈剂量依赖性(P<0.;01);
- RESULTS: Under the optical microscope, mouse embryonic fibroblasts were irregular, rapidly grew, passaged at 1:4.Following mitomycin C treatment, cells slowly adhered and could not proliferate. 结果:光镜下小鼠胚胎成纤维细胞呈不规则形,生长较快,传代比例为1:4,经丝裂霉素C处理后细胞贴壁较慢,失去分裂增殖能力。
- Nonetheless, Daley said the discovery shouldn't be used as a replacement for human embryonic stem cell research. 不过,Daley博士也说,人类胚胎干细胞研究不宜因为此次羊水干细胞的研究发现而就从此偃旗息鼓。
- Spontaneous Differentiation of Ectomesenchymal Stem Cell of Human Embryonic Facial Process in Vitro. 面突外胚间充质干细胞在体外的自发分化。
