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- Conclusion Highly-active recombinant mutant human annexin V with endogenous metal-chelating sites can be expressed in Pichia Pastoris system. 结论 利用毕赤酵母系统表达出高活性的、具有内在金属螯合位点的突变型人膜联蛋白V。
- Objective To evaluate the efficacy of ~(99)Tc~m labeled recombinant human Annexin V(~(99)Tc~m- rh-AnnexinV)in evaluating apoptosis after radio-or chemotherapy in Hca-F25(hepatoma cell line)mice mod- els. 目的验证~(99)Tc~m 直接法标记的重组入膜联蛋白 V(~(99)Tc~m-rh-Annexin V)用于早期评价放化疗后肿瘤组织细胞凋亡状态的可行性。
- Human Annexin V 人膜联蛋白V（HA-V）
- human annexin V(HAV) 人膜联蛋白（HAV）
- The FasL expression was positively correlated with the positive rate of annexin V labeling and Fas expression on tumor cells killed by the LAK cells. LAK细胞致肿瘤细胞死亡中FasL表达与annexin－V标记阳性率和肿瘤细胞表面Fas表达正相关。
- Mdthods MTT assay and annexin V staining were used to observe cell proliferation and apoptosis rate in AM-treated K562 cells. 方法采用MTT检测观察细胞增殖变化,膜联蛋白V染色观察细胞凋亡率。
- The damage of mouse brain after microinjection was examined by flow cytometry using PI stain or Annexin V plus PI stain. 无论是从脑干或海马迴感染，鼠脑的脑干皆有超过百分之十的损伤死亡；
- Methods Morphological study, DNA gel electrophoresis, flow cytometry and fluorescin labeled Annexin V to detect apoptosis, RT PCR to detect the gene related with apoptosis. 方法 应用形态学方法 ,流式细胞术 (FACS) ,DNA凝胶电泳等方法检测细胞凋亡的发生 ,应用RT PCR检测凋亡相关基因Fas与FasL的变化。
- Methods Morphological study,DNA gel electrophoresis,flow cytometry and fluorescein labelled annexin v were used to detect apoptosis,RT PCR to detect the apoptosis related gene. 方法 应用形态学、流式细胞术、DNA凝胶电泳、AnnexinV标记等方法检测细胞凋亡的发生 ,应用RT PCR检测凋亡相关基因Fas、bcl 2、c myc表达的变化。
- The specific binding of phosphatidylserine exposed on the cell surfaces undergoing apoptosis with annexin V or lactadherin,can be used for early detection o. 因此,通过膜连蛋白V或乳凝集素与凋亡细胞膜表面外翻的磷酯酰丝氨酸特异性结合,可用于冠状动脉易损斑块的早期检测。
- Motheds: Apoptosis of bone marrow cells were measured using methods of DNA content and Annexin V by flow cytometer (FCM) in 48 acute leukemia, 14 MDS, and 16 non-leukemia and non-MDS anemia patients, and the two methods were compared. 方法:采用流式细胞术DNA含量和(或)膜联蛋白(Annexin V)法检测了48例急性白血病(AL)、14例MDS及16例非白血病、非MDS贫血患者骨髓细胞的凋亡,并对上述两种凋亡的检测方法进行相关性比较。
- Prourokinase (144 411) Annexin V Chimeras fuse gene was inserted into Bombyx mori baculovirus transfer vector pBacPAK8 and co transfected with lineared DNA of Bm BacPAK6 virus into BmN cells. 将低分子量尿激酶与膜联蛋白 (AnnexinV)的融合基因重组到家蚕杆状病毒转移载体pBacPAK8中 ,获得重组转移载体pBacPAK UKAV ,并与线性化的病毒Bm PAK6DNA共转染家蚕细胞 ,获得重组病毒BacPAK UKAV。
- Keywords Liver injury;Apoptosis;Annexin V;PI; 关键词肝损害;脱噬作用;膜联蛋白V;碘化吡啶;
- It's only human nature to want a comfortable life. 想过舒适的生活不过是普通人的本性。
- Keywords annexin V;mutation;expression;Pichia Pastoris; 膜联蛋白V;突变;表达;毕赤酵母;
- Keywords Parkinson disease;Annexin V;apoptosis;radioactive tracer; 关键词帕金森病;膜联蛋白V;凋亡;放射性示踪剂;
- Keywords Vulnerable plaque;Radiolabeled annexin V;Rabbit; 不稳定斑块;放射标记的膜联蛋白;兔;
- Speeches distinguish human beings from animals. 人类和动物的区别在于人会说话。
- The war took a heavy toll of human life. 这次战争夺去了许多人的生命。
- Perhaps it is our human nature to be nostalgic. 也许是人类天生有一种思古之幽情。
