您要查找的是不是：
- Objective To construct recombinant adenovirus by homologous recombination in bacteria. 目的通过细菌内同源重组方法构建重组腺病毒。
- Objective To efficiently construct the recombinant adenov ir us containing CD/TK fusion gene driven by vascular endothelial growth factor (V EGF) promoter using improved homologous recombination in bacteria. 目的应用简化的细菌内同源重组法高效制备含血管内皮细胞生长因子(VEGF)启动子驱动的CD/TK融合基因重组腺病毒。
- The recombinant adenovirus vector, pAdCMV-IL3-Endo, was cotransfected into 293 cells together with pBHG10 by lipofectamine, and recombinant adenovirus, which named Ad-ILs-Endo, were generated by homologous recombination. 然后用脂质体法将上述穿梭质粒与质粒pBHG10共转染293细胞，经同源重组获得复制缺陷型重组腺病毒Ad-IL_3-Endo。
- Increasing the Transient Expression of GUS Gene in Plant Tissue by Homologous Recombination. 利用同源重组提高外源GUS基因在植物组织中表达。
- Based on homologous recombination, a recombinant PRV co-expressing protein precursor P1-2A of FMDV and VP2 protein of PPV had been constructed using PRV TK7gE7LacZ+ mutant as the vector virus. 采用脂质体介导法将pIEP1-2A-3C与伪狂犬病病毒基因缺失标志疫苗株PRV TK~-/gE~-/LacZ~+基因组共转染,构建了表达口蹄疫病毒P1-2A和3C基因的重组伪狂犬病病毒TK/gE~-/P1-2A-3C,并用空斑筛选与PCR鉴定的方法纯化重组病毒。
- AV1. SpaT-hIGF-1 was synthesized by homologous recombination of pDNR-hIGF-1 and pInt. AV1.;SpaT同源重组形成pInt
- Objective To test the anti-tumor effect of adenovirus vector generated from the homologous recombination of bacteria mediating TK gene on hepatic neoplasm cell. 目的探讨用细菌内同源重组法制备含TK自杀基因腺病毒对肝癌细胞的杀伤作用。
- The mutant 31BK was engineered, in which homologous recombination of the aroBKanr gene cassette into the aroD locus(aroD::aroBKanr)of the E. 构建宿主菌基因精确定位突变株31BK(aroD::aroBKan~r)
- Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 酵母基因中断技术是研究酵母基因功能的重要手段，自80年代初诞生以来经历了不断的改进和发展.
- We start with these fragments, and then we have a homologous recombination system that reassembles those into a chromosome. 我们以这些片段开始，然后通过同源重组将其组合到染色体中。
- Abstract： Gene disruption by homologous recombination is a powerfu l tool for investigating gene function in yeast.Since 1980’s, it has been deve loped a lot. 摘 要： 酵母基因中断技术是研究酵母基因功能的重要手段，自80年代初诞生以来经历了不断的改进和发展.
- Most members of the XRCC genes family participated in several DNA repair pathways, including base excision repair, homologous recombination repair and non-homologous end joining. 目前已鉴定的这一基因家族的多数成员均参与几种重要的DNA修复途径,包括碱基切除修复、同源重组修复和非同源末端重接。
- Each open reading frame (ORF) is knocked out using a PCR-based gene deletion strategy that takes advantage of the high degree of homologous recombination in yeast. 每个开放读码框使用基于PCR基因删除策略,使用在酵母中的高度同源重组优势。
- Keywords B7-H4;gene engineering;homologous recombinant; 基因工程;同源重组;
- The amplified gene was cloned into the pENTR11 vector. With the pENTR11-SLC plasmid and the backbone plasmid pAd/CMV/V5-DEST, the homologous recombination reaction took place in vitro. The reaction mixture was transferred into TOP10 E. 方法采用PCR技术从含有SLC基因的质粒上扩增出SLC基因,将PCR产物酶切后连接至pENTR11载体上,再通过pENTR11与腺病毒骨架载体pAd/CMV/V5-DEST之间的同源重组作用将SLC基因片段重组至pAd/CMV/V5-DEST上,最后经293细胞的包装扩增后得到携带SLC基因的重组腺病毒。
- In S. coelicolor, the rapid increase of SCP2? plasmid conjugation efficiency and that of plasmid homologous recombination efficiency associates with development of aerial mycelium. 在天蓝色链霉菌中，SCP2质粒接合转移频率的快增长，SCP2质粒介导的质粒同源性重组频率的快增长与气生菌丝的形成同步。
- Though RNA dot hybridization is negative, OP J18-1400 has a conservative domain of seven base pairs DNA sequence: 5-TTCCCTC-3, which is a homologous recombination hotspot domain in atp6 gene. 虽然OPJ18－1400对应的RNA斑点杂交呈阴性，但其DNA序列中存在一个七碱 基5－TTCCCTC－3的保守系列，它是atp6基因中同源重组热点区，其促使线粒体基因重组形成嵌
- Diploid individuals can repair a mutated section of its DNA via homologous recombination, since there are two copies of the gene in the cell and one copy is presumed to be undamaged. 二倍个体可以通过同源重组，修复它DNA的一个突变片段，因为在细胞中有两个拷贝的基因，其中一个拷贝假定为未被损坏的。
- PCR Detecting Homologous Recombinants from ES Cell PCR对ES细胞定点整合重组子的鉴定
- Homologous recombination in bacteria 细菌内同源重组
