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- Results Of the 23 mutations, 22 were detected by heteroduplex analysis (96%), 20 by SSCP analysis (87%) and 23 by heteroduplex-SSCP analysis (100%). 结果23个杂合子突变中,异源双链法、SSCP法与异源双链-SSCP法检出突变分别为22个(96%25)、20个(87%25)和23个(100%25)。
- Methods The microsatellite polymorphism of MICA exon 5 in 175 unrelated healthy individuals were investigated using PCR heteroduplex analysis. 方法用PCR-异源双链分析法,对175名正常无关个体的MICA基因第5外显子微卫星多态性的分布进行研究。
- Conclusion Heteroduplex-SSCP analysis is simple, economical and more effective than SSCP or heteroduplex analysis alone and it may be widely applied in mutational screening. 结论异源双链-SSCP法突变检出率高,经济、简便,值得在基因突变筛查中推广应用。
- Results from heteroduplex analysis are more stable and repeatable than those of SSCP and they may complement each other.Heteroduplex-SSCP analysis has the advantages of heteroduplex and SSCP analyses. 异源双链法结果比SSCP法结果稳定,重复性好,两种方法之间有一定互补性,异源双链-SSCP法兼有两种方法的特点。
- COMPARISON OF HETERODUPLEX ANALYSIS AND SSCP IN GENE MUTATION SCREENING 异源双链分析和单链构象多态性分析在基因突变检测中的作用
- Keywords MICA gene;human leukocyte antigen;microsatellite;heteroduplex analysis;linkage disequilibrium; 关键词MICA基因;人类白细胞抗原;微卫星;异源双链分析;连锁不平衡;
- One who draws blood for analysis or transfusion. 放血医师为化验或输血而抽血的人
- Your critical analysis helped me a great deal. 你的评论分析对我帮助很大。
- The metal was submitted to analysis. 对该金属进行了分析。
- Her analysis was correct to the nail. 她的分析极其正确。
- Textual analysis identified the author as Shakespeare. 对原文的分析研究鉴定出作者是莎士比亚。
- The cake on analysis was found to contain traces of toxin. 经分析发现蛋糕里含有微量的毒素。
- Close analysis of sales figures showed clear regional variations. 对销售额的仔细分析显示出明显的地区差别。
- heteroduplex analysis 异源双链分析
- We agreed with his acute analysis of the political situation. 我们都同意他对政治形势的深刻分析。
- heteroduplex analysis(HA) 异源双链分析
- Close analysis of sales figures shows clear regional variations. 对销售额的仔细分析显示出明显的地区差别。
- The analysis showed a few grains of arsenic in the solution. 分析显示溶液中有几格令的砷。
- Analysis showed that cocaine was present in the mixture. 分析的结果表明混合物中含有可卡因。
- Keywords chimpanzee;hepatitis C virus;human immunodeficiency virus;heteroduplex analysis;nonsynonymous substitution;single-stranded conformational polymorphism;synonymous substitution; 黑猩猩;丙型肝炎病毒;人免疫缺陷病毒;异源双链分析;单链构象多态;非同义替换;同义替换;