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- Methods: EPF was purified from 430ml human pregnancy sera. The purification scheme involved sequential DEAE 52 chromatography, SP Sepharose F. F chromatography, ConA Sepharose 4B chromatography and Heparin Sepharose C1-6B chromatography. 方法:从430ml早孕血清中采用柱层析方法分离纯化EPF,依次经过DEAE 52阴离子交换层析、SP Sepharose EF阳离子交换层析、ConA Sepharose 4B亲和层析和Heparin Sepharose C1-6B亲和层析。
- Keywords aFGF;bovine brain;cation exchange chromatography;Heparin Sepharose affinity chromatography;3T3 cell; 牛脑;离子交换层析;琼脂糖凝胶亲和层析;3T3细胞;
- heparin sepharose 肝素琼脂糖(透)
- Heparin is the treatment of choice. 肝素为首选的治疗剂。
- After purification with DEAE Sepharose F. 结果：成功得到海螵蛸粗多糖。
- Human heparin sulphate protoglycans，HSPG Elisa ... 人硫酸肝素糖蛋白（HSPG）试剂盒；
- Heparin is not intended for intramuscular use. 肝素是不打算肌肉注射使用。
- Heparin is the most widely used and reliable anticoagulant. 肝素为最广泛应用及最可靠的抗凝剂。
- To discuss the nursing maneuver of hemodialysis without heparin. 目的探讨无肝素血液透析的护理策略。
- Baxter accounts for about half the U.S. supply of heparin. 巴克斯证实大约一半肝磷脂由美国供应。
- Exceptio include phenytoin, theophylline, and heparin. 只有苯妥英、茶碱和肝素是例外。
- Exceptions include phenytoin, theophylline, and heparin. 只有苯妥英、茶碱和肝素是例外。
- Its homogeneity was identified with Sepharose 6B column chromatography. 紫外光谱显示它不含蛋白质、多肽及核酸。
- All cases received heparin and urokinase perioperatively. 围手术期抗凝治疗。
- The results suggest that heparin could interrupt AGE clearance... 低分子量肝素对AGE受体无封闭作用。
- Heparin-induced thrombocytopenia occurring after discontinuation of heparin. 肝素停药后发生的肝素相关性血小板减少。
- Serum phosphate levels were elevated throughout the heparin calcium therapy. 整个肝素钙治疗过程中，患者的血磷水平都升高。
- The penetration rates of MPS were between 6 and 50 times of those of heparin. MPS（多磺酸粘多糖）的透皮吸率是肝素的6－50倍。
- Made sheep anti?human IgG Fab Sepharose 4B affinity chromatography, purified the souble Fab fragment. 制备羊抗人IgGFab抗血清、建立抗人IgGFab抗体－Sepharose4B亲和层析柱，纯化Fab片段。
- Conclusion Heparin could make PLT and MPV decreased, especially in UFH group. 结论肝素抗凝可致PLT及MPV下降,UFH尤其显著;
