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- A pair of general primer (ITS5-ITS4) was used to test in amplification of internal transcribed spacer (ITS) region of chromosomal DNA of fruiting bodies of Boletus edulis in Yunnan. 利用ITS的通用引物(ITS5-ITS4)对云南的美味牛肝菌(Boletusedulis)子实体的DNA进行PCR扩增,扩增产物回收后直接测序。
- Then , partial eDNA of Luffin ~2 was cloned by RT-PCR with two primers ( one was degenerate primer for 5?terminal primerand the other was a general primer containing oligo-dT). N－端 9个氨基酸的序列，并据此设计5，端简井引物，用此引物和 3’－端通用引物，通过RTFCR技术克隆了 Luffin S。 的部分cDNA序列。
- All of the 51 Bt were analyzed by the PCR method with general primers of cry1, cry2, cry3, cry4, cry-n, cry7, cry8, cry11, cry13 and cyt genes. 对51 株Bt 分离菌株的cry 基因进行了PCR 鉴定。
- Gunner Jones was praised by the general. 炮手琼斯受到了将军的表扬。
- Please give me a general idea of the work. 请告诉我这项工作的梗概。
- My general impression was that it was quite good. 大致上我觉得那相当好。
- The general decided to fly up to see his soldiers. 将军决定飞向北部去看看他的士兵们。
- Mr Powel has marked out a general course of action. 鲍威尔先生已经拟定了一个总的行动计划。
- The general has taken the helm of the states. 这位将军掌握了国家领导权。
- He was appointed the general consul of Japan. 他被任命为驻日本的总领事。
- He was exalted to the position of general manager. 他被提升到总经理的职位。
- The proposal was met with general acceptance. 这建议得到普遍的赞同。
- In the army, the general takes rank of the colonel. 在军队里，将军较上校有优先权。
- The general effect of the painting is overwhelming. 这幅画给人总的印象是很有气势。
- The general's speech infused keenness into the men. 将军的演讲给人们注入了激情。
- The rebels returned home under a general amnesty. 造反的人经大赦释放回家。
- The general told the troops to lay down their arms. 将军命令部队放下武器。
- The general's uniform was trimmed with gold braid. 将官的制服饰有金色的穗带。
- The king raised his general to the peerage for winning the war. 由于赢得了这场战争，国王封他的将军为贵族。
- The word has attained general currency. 这个词已经通用起来了。
