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- By the BP recombination reaction,the PCR product containing attB was transfered to an attP-containing donor vector to create an entry clone. Finally,DREB1A gene was shutted into pH2GW7 vector by LR recombination reaction. 通过BP反应将包含有attB接头的PCR产物克隆到含有attP的donor载体上以产生Entry克隆,通过LR反应将已经重组入Entry载体的DREB1A基因再克隆到pH2GW7双元载体。
- Three indica male sterile lines, K-qing A, K19A, and K17A, have been bred successively by using sterile plants from the progeny of a hybrid cross between japonica and indica as cytoplasmic male sterile (CMS) gene donor. 利用粳籼杂交后代出现的不育株作细胞质雄性不育供体，先后育成了K青A、K19A和K17A等籼型不育系。
- gene-donor vector pTLG 基因供体pTLG
- Methods The ORF2 gene of TTV was amplified from the serum of a blood donor in Lanzhou region by nest PCR and inserted into vector pGEM-T then,after identification by DNA sequencing,subcloned to expression vector pET22b(+). 方法通过巢式PCR,从一份兰州地区无偿献血员血清中扩增TTV病毒的ORF2基因,并克隆到pGEM-T载体中,进行核苷酸序列分析。
- Resistance gene donor 抗性基因供体
- The construction of recombinant vector containing Smac/ DIABLO gene. 构建含有Smac/DIABLO基因片断的重组质粒pcDNA3.;1 /HisA-Smac/DIABLO。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- As a mammalian gene transfer vector, baculovirus owns some unique advantages. 杆状病毒作为哺乳动物细胞基因转移的载体有很多独特的优点。
- The position that a given gene occupies on a chromosome. 基因座在一条染色体上某种给定基因所占的位置
- Half of the people who inherit the gene express it. 半数有这种遗传基因的人，皆显示出其影响
- Now the variant of each gene is called an allele. 每一种基因的变化就叫做等位基因。
- We know a lot about the structure of gene now. 如今我们对基因的结构有了较多的了解。
- A single gene may have many effects. 单一基因可能具有很多种效应。
- There can be more than two alleles for a gene. 一个基因可能有两个以上的等位基因。
- A mutable organism; a mutable gene. 一个易变的生物；一个易变的基因
- As presented in has high degree of apomixis, E. binata might act as a potential valuable donor for apomixis gene . 龙须草具有很高的无融合生殖度，是优良的无融合生殖基因供体。
- Gene Shepard: That was a 35 dollar belt! 吉恩·谢帕德：那地方可值35美元呢！
- Gene Shepard: That was a35 dollar belt! 吉恩·帕德：那地方可值35美元呢！
- You also owe a nod to your leptin gene. 你还要赞美你的leptin基因。
- Oncogene: Gene that can cause cancer. 致癌基因: 能够导致恶性肿瘤的基因。
