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- The esat6-mpt64 fused gene can be expressed in COS-7 cell. 结论:构建了真核表达载体pcDNA-esat6-mpt64,并在真核COS-7细胞中表达。
- The RT-PCR methed was used to amplify bcr/abl fuse gene of CML. 采用RT-PCR法检测CML细胞ber/abl融合基因。
- The fused gene STI-STII-LTB was cloned into plant expression vector pBI121-MARs to replace GUS gene, another plant expression vector was constructed without MAR sequence. 将融合基因STI-STII-LTB构建到带有核基质结合区序列(MARs)的植物表达载体pBI121-MARs中GUS基因的位置。
- The recombinant plasmid containing ATF-PAI2CD fused gene, pZWY-ATF-PAI2CD, was transferred to the competent cells of yeast strain GS115. After the phenotype selection, the positive clones were induced with methanol to express ATF-PAI2CD. 该质粒转化毕赤酵母菌GS115 ;用G4 18 YPD平板筛选高拷贝转化子 ;然后用甲醇诱导表达 .;工程菌用摇瓶发酵;表达产物ATF PAI2CD占培养液中总蛋白 5 0%25以上
- Prourokinase (144 411) Annexin V Chimeras fuse gene was inserted into Bombyx mori baculovirus transfer vector pBacPAK8 and co transfected with lineared DNA of Bm BacPAK6 virus into BmN cells. 将低分子量尿激酶与膜联蛋白 (AnnexinV)的融合基因重组到家蚕杆状病毒转移载体pBacPAK8中 ,获得重组转移载体pBacPAK UKAV ,并与线性化的病毒Bm PAK6DNA共转染家蚕细胞 ,获得重组病毒BacPAK UKAV。
- Construction and expression of cpcB-CT fused gene in E. Coli cpcB与CT融合蛋白表达质粒在大肠杆菌中的表达和纯化
- Lead will fuse at quite a low temperature. 铅会在相当低的温度下熔化。
- Genetic Transformation of Carrot with Diarrhea Antigen Fused Gene CTB-CS3 腹泻抗原融合基因CTB-CS3对胡萝卜的遗传转化
- It seems that you've blown a fuse. 看样子你把保险丝烧断了。
- We fused the pipes with solders. 我们用焊锡熔接管子。
- reST Ⅰ - linker - LTB fused genes mSTⅠ-linker-LTB融合基因
- Containing molecules consisting of two fused rings. 二环的包含由两个结合的环组成的分子的
- Changing a fuse is one of those fiddly jobs I hate. 我最不愿意干换保险丝这类活儿了。
- Don't irritate her, she's on a short fuse today. 别惹她，她今天动不动就发火。
- A short circuit will blow the fuse. 短路会烧断保险丝的。
- We fuse copper and tin to make bronze. 我们把铜和锡熔制成青铜。
- He struck a match and ignited the fuse. 他划了根火柴，点著了导火索。
- He is famous for his nasty temper.He has a very short fuse. 他脾气坏是出了名的。他很容易激动。
- We disagreed at first but eventually our ideas fused. 我们的意见开始时有分歧,但最後取得了一致。
- He fused all the lights by using his electric stove. 他因用电炉,烧断了保险丝,电灯全都熄灭。
