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- Two EST(One come from hemin induced HEL cells while the other come from PMA induced HEL cells) were chosen as the probes for cDNA library screening and several full length cDNA clones were isolated from human bone marrow cDNA library. 选择两个新EST筛选人骨髓细胞cDNA文库，获得两个新的锌指蛋白编码基因的全长cDNA，分别命名为HZF1(其EST来源于被hemin诱导的HEL细胞)和HZF2(其EST来源于被PMA诱导的HEL细胞)。
- The Methods of Constructing the Full Length cDNA Library 全长cDNA文库的构建方法
- full length cDNA library 全长cDNA文库
- full- length cDNA library 全长cDNA文库
- Objective: To get human CD40 gene full length cDNA sequence and to express functional CD40 molecule in COS 7 cells. 目的：克隆编码人CD40 分子基因的全长cDNA，在COS－7 细胞中获得高表达。
- The full length cDNA of ANGPTL4 consists of 1943 nucleotides in which there is a 1218 bp ORF encoding a 406-amino acid polypeptide (predicted molecular mass is 45.2 kDa). 并用RACE-PCR获得其全长。该基因cDNA全长为1943 bp，开放阅读框含1218 bp，编码406个氨基酸，预测分子量为45.;2 kDa。
- The full length cDNA of the genome segment (S9) of Rice gall dwarf virus Xinyi isolate (RGDV-C) was cloned and the complete nucleotide sequence was determined. 应用RT-PCR技术克隆了水稻瘤矮病毒(RGDV)中国广东信宜分离物(RGDV-C)的基因组S9片段，测定了全序列并进行了生物信息学分析。
- An Improved Method for Solid-phase cDNA Library Construction. 一种改进的cDNA文库固相构建法。
- Construction of cDNA library from Sophora alopecuroides L. 苦豆子cDNA文库的构建。
- Some quality analysis, such as the titer, the recombinant rate and insert cDNA length of the cDNA library, indicated that the library contained intact genes and could be use... 广西大学亚热带生物资源保护和利用实验室;
- The full length cDNA of Rtn-T was 3491 bp and encodes a 108 kDa protein of 986 amino acids. This Rtn-T has Genbank accession number AF333336. Blast search in the human genome datebase showed that Rtn-T consisted of 9 exons. 序列测定显示Rtn-T cDNA全长为3419bp，编码蛋白为108KDa，含986个氨基酸，该基因已被Genbank收录，收录号为AF333336。
- In this study, the full length cDNA and DNA of pollen development related BcMF2 and BcMF4 gene was cloned and characterized from Chinese cabbage pak-choi (Brassica campestris L. ssp. chinensis Makino). 本研究以BcMF2和BcMF4基因为研究对象,通过PCR扩增方法对白菜BcMF2和BcMF4基因的基因组全长和cDNA全长进行了克隆、鉴定以及蛋白质结构和功能的预测;
- Construction and Analysis of cDNA Library from the Leaf of Coleus blumei Benth. 彩叶草叶片cDNA文库的构建与分析。
- Methods Screening human placenta cDNA library with rat C/EBP cDNA probe. 方法以大鼠C/EBPcDNA为探针，筛选人胎盘cDNA文库。
- Construction of cDNA library of peripheral blood leucocytes in normal Cyprinus carpio L. 正常鲤外周血白细胞cDNA文库的构建。
- RNA was extracted from a white young alpaca and the full-length cDNA library was constructed by SMART technology. 从白色青年羊驼皮肤提取总RNA,并分离mRNA,利用SMART技术,构建了羊驼皮肤全长cDNA 文库。
- A high throughput in silico data analysis system for cDNA library, CLASP,was constructed by bioinformatic approaches. 应用生物信息学方法,构建了一套针对cDNA或EST文库的高通量、自动化分析体系,CLASP(cDNA Library Analysis SystemPrimary)。
- The cDNA library of the liver and kidney from the Vibrio anguillarum-infected Haliotis discus Hannai was constructed. 用鳗弧菌(Vibrio anguillarum)人工感染皱纹盘鲍(Haliotis discus Hannai),取等量肝和肾组织混匀,Unizol提取总RNA。
- A1200 and pRT! 05. The Suuccki cDNA library were driven by the cauliflower mosaic virus (CaMV) 35S promoter. 过量表达载体由pCAMBIA1200和pRT105组装而成，使得cDNA文库在CaMV35S启动子作用下过量表达。
- Full-length cDNA pool is the most important step in construction of cDNA library. 而全长cDNA池(full-length cDNA pool)则是cDNA文库构建的最重要环节。
