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- In this experiment PST lipsome was produced by freeze thawing method. 采用冰冻熔融法制备脂质体,包封率进行测定可达29%25。
- Methods The tumor antigen peptides from 4T1 breast cancer cells were acquired by freeze thawing method and HSP70-4T1 peptides complex was reconstructed in vitro. 方法冻融法获取BALB/C小鼠4T1乳腺癌细胞抗原肽,体外重组HSP70-4T1抗原肽复合物。
- Freezing thawing durability of both ordinarily and air entrained concrete exposed to Xinjiang, Qinghai, Inner Mongolia and Xizang salt lakes were determined by the accelerated freezing thawing method. 用快冻法研究了普通混凝土与引气混凝土在水和西藏、内蒙古、新疆和青海盐湖卤水中的抗冻性。
- The CMO ORF was cloned and the plant expression vector pBI121-CMO was constructed. The resultant plasmids pBI121-CMO was transferred into Agrobacterium tumefaciens (GV3101) by the liquid nitrogen freezing thaw method. 克隆了CMO基因的编码区,构建了其植物表达载体pBI121-CMO,冻融法将质粒导入根癌农杆菌(Agrobacterium tumefaciens)GV3101,获得工程菌GV3101-pBI121-CMO。
- Keywords tanshinone liposomes;freeze thawing method;cryoprotectant;particle size; 丹参酮脂质体;冻融;冷冻保护剂;粒度;
- pBinAR-aPG and pBinAR-PG were transferred into Agribacturillus EHA105 respectively by freeze thawing method which could be used for further transformation. 把两个表达载体pBinAR-aPG和pBinAR-PG通过冻融法分别导入农杆菌EHA105感受态,为进一步遗传转化作准备。
- The effects of PVP in formulation and freeze thawing steps on drug leakage and aggregation of tanshinone liposomes were investigated. 考察了聚乙烯吡咯烷酮(PVP)以及冻融处理对丹参酮脂质体的药物渗漏和微粒聚结特性的影响。
- This artic le has discussed the advantage of different kinds of prepartions:reverse_phase e vaporation, crush, freeze thawing . 通过对其包裹率的测定，结果表明：反相蒸发法包裹率高，反相蒸发法为最佳工艺。
- The paper studied the Solubilizing effect of solvent extraction, freeze thawing, ultrasonication, phosphoric acid treating and enzymatic hydrolysis on Spirulina dried Powder. 对溶剂浸提，反复冻融，超声波处理，磷酸处理和酶解技术对螺旋藻粉的增溶效果进行了比较。
- A dynamic module damage model was built to predict the dynamic strength and maximum dynamic module of the silty clay after every freeze thaw cycle. 建立了动模量损失模型，能够预测每一次冻融循环后的动强度和最大动模量。
- Reach the Conclusion that the freeze thawing extraction, and Centrifugation, and then enzymatic hydrolysis of the filter residue is the optimum solubilization methot. 发现以反复冻融浸提，其渣再酶解处理为最佳增溶方法。
- In this paper, the thawing methods and their characteristics were introduced. 这里对各种解冻方法及其特点进行了分析。
- The built prediction model is characterized by high precision, can be used to evaluate the effect of the freeze thaw cycles on the service life of the silty clay roadbed. 通过与试验对比可知，预测模型具有较高的精度，可以用来评估冻融循环对粉质黏土路基使用寿命的影响。
- Methods:The tests about the three key techniques:cryoprotective medium,freezing and thawing methods of Capella?s method were comparatively studied. 方法 :对经典的 Capella四步冻贮法的冷冻保护液、降温冷冻速率和复温方法 3项关键技术做了全面简化和改进。
- The cryopreservation and thawing method of porcine oocytes were reviewed.The effect and influence factors of preservation, choosing protection agents and so on were also discussed. 摘要综述了猪卵母细胞的冷冻保存方法和解冻方法，同时对保存效果、影响保存的因素、保护剂的选择等进行探讨。
- Based on a lot of animal experiments,scientists have developed two kinds of cryopreservation programme:slow cooling-fast thawing method(two step method),fast cooling-fast thawing method. 经过大量动物实验，人们建立了两种冷冻程式：慢速冷冻-快速复温法（两步法）；快速冷冻-快速复温法。
- Objective To investigate the anti-chronic myeloid leukemia(CML) effect of cytotoxicity T lymphocytes(CTL) activated by dendritic cells(DC) pulsed with freeze thaw K562 lysates antigan. 目的研究K562冻融抗原负载的健康供者来源的树突状细胞(DC)诱导细胞毒性T淋巴细胞(CTL)体外杀伤慢性粒细胞白血病(CML)细胞的毒性效应。
- The effects of the hydrophilic monomer AA and initiator K 2S 2O 8 on the electrolyte stability, freeze thaw stability and mechanical stability for the copolymer(MMA/BA/AA) soap free emulsion(pH=8)have been studied. 研究了亲水性单体AA和引发剂K2 S2 O8用量对三元共聚物 (MMA/BA/AA)无皂乳液 (pH =8)的电解质稳定性、冻融稳定性和机械稳定性的影响。
- The different thawing methods have different characteristics.The suitable thawing method should be selected to different material and different use of same kind material. 不同的解冻方法具有不同的特点,对于不同的物料和同一种物料解冻后的用途不同,应选择合适的解冻方法。
- On the basis of the results obtained from comparison teats of fly ash soils and silty clay on dynamic strength under freeze thaw cycles, the dynamic strength was quantitatively analyzed. 摘要根据粉煤灰土和粉质黏土经过冻融循环后动强度的对比试验，对粉煤灰土和粉质黏土的动强度做出了定量分析。
