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- Morphological change of macrophages observed with transmission electronmicroscopy and fluorescence staining. 然后利用透射电镜和荧光染色观察凋亡巨噬细胞的形态学特征;
- Furthermore, the induction of apoptosis by CSE in HepG2 cells was observed with AO/EB fluorescence stain. 0mg/mL赤参水提物处理HepG2细胞24h后,细胞荧光染色出现了明显的凋亡细胞;
- The apoptosis of suspension cultured cells of Viola diffusa Ging. which had been induced by Ce(NH4)2(NO3)6 were detected by DNA Laddering and DAPI fluorescence stain methods. 用DNA Laddering和DAPI荧光检测法对Ce(NH4)2(NO3)6诱导的蔓茎堇菜(Viola diffusa Ging.;)
- BHT could induce apoptosis to pulmonary macrophage in the perfusate of isolate perfused rat lung as examined by the technology of PI fluorescence stain and comet assay. 结论亚急性BHT大剂量染毒可造成大鼠肺、肝等脏器功能性和器质性损伤,BHT可诱发肺巨噬细胞凋亡的产生。
- The double FITC-UEA-I and Dil-ac-LDL fluorescence staining displayed differentiated EPCs. The expression of VEGF was found in the medium of EPCs transfected with pcDNA3hVEGF165.VEGF165 gene transfection could stimulate EPCs proliferation. FITC-UEA-I和DiI-ac-LDL荧光双染证实分化的EPCs,脂质体介导pcDNA3-hVEGF165质粒转染组EPCs培养基上清中表达VEGF,hVEGF165基因转染促进EPCs增殖,注射转基因EPCs的大鼠皮下局部血管增加。
- MTT killing experiment, Bradford protein determination method, cell growth curve experiment and HE staining, fluorescence staining were used to study the effects of AP extract on tumor cell lines. 采用MTT杀伤实验、Bradford蛋白测定法、HE染色和荧光染色法,观察杏多糖粗提物对体外培养的五株肿瘤细胞(SPC-A-1、Q_3、HeLa、MGC-803、K562)影响作用。
- By using fluorescence staining method, the effect of receptor-mediated endocytosis on intracellular pH (pHi) of mouse macrophages was studied with adherent cell analysis and sorting cytometer 570 (ACAS 570). 用pH值敏感荧光探针Snaflcalcein-AM标记体外培养48小时的小鼠腹腔巨噬细胞,570型粘附式细胞仪观察巨噬细胞在对刀豆素A进行受体介导内吞过程中细胞内pH值的变化。
- These hair cells were fluorescence stained with 0.01% euchrysine and observed under fluorescence-microscope. 结果:凋亡毛细胞的主要形态特征包括:胞核皱缩;
- Methods Indirect fluorescent staining, FCM analysis and RT PCR were employed to detect the expression and regulation of PTA1 at the levels of PTA1 protein and PTA1mRNA. 用RT PCR方法检测内皮细胞中PTA1mRNA的表达 ;
- Then these biofilms were visualized by fluorescence stainning with LIVE/DEAD bacterial vitability kit and were observed by confocal laser scanning microscopy (CLSM). 生物膜进行萤光染色,镭射共聚焦扫描显微镜(CLSM)观察。
- He vainly tried to mark the defacing stain out. 他试图除去有损外观的污迹,但没有成功。
- I put the shirt in soapy water to soak out the stain. 我将衬衫浸在肥皂水里以去除污渍。
- I scrubbed hard at the stain to see if I could get it off. 我使劲擦这个污迹,看能不能将它去掉。
- Nonspecific and specific phagocytic activities of macrophages with heterogeneous fluorescent staining were observed using starch polysaccharide and immune activators (diphtheria toxoid and BCG) to induce and activate mouse peritoneal macrophages. 利用淀粉多糖和免疫促进剂(白喉类毒素和卡介苗)诱导和活化小鼠腹腔巨噬细胞,观察了四种异质性荧光染色的巨噬细胞非特异性和特异性吞噬活性。
- Immunoreactivities for Fas, FasL and caspase - 3 in-creased considerably in the cervical carcinoma tissue. Dual positive fluorescent staining for ap-optosis and caspase - 3 or Fas was appeared in some cells. 癌组织中,Fas、FasL、caspase-3蛋白阳性细胞明显增多,双重荧光染色显示,部分细胞Fas及cspase-3蛋白与凋亡细胞同时阳性染色。
- MTT method was employed to detect the proliferation of cells,while the adhesive ability was investigated by stereology. In the meantime,the cell integrity and the interface between biomaterial and cells were observed by fluorescent staining and SEM. 采用MTT法检测细胞增殖活性,体视学方法检测细胞粘附能力,荧光双染法检测细胞完整性,扫描电镜观察细胞-材料界面。
- I am afraid you must wash out this stain. 恐怕你必须洗掉这个污渍。
- The stain on his shirt is difficult to clean off. 他衬衣上的污点很难去除掉。
- Methods For 23 cases (38 eyes) diagnosed of keratoconjunctivitis sicca, conjunctival impression cells were examined by using acridine orange fluorescent staining, the results being evaluated and graded by Tseng Classification. 方法选自我院眼科门诊已确诊的干燥性角结膜炎患者23例(38眼),用丫啶橙荧光染色结膜印迹细胞检查法进行检测,并用结膜印迹细胞检查结果评价标准进行评价,按Tseng分类法进行分级。
- Methods: IRE-EGFP-esp1 plasmid was transfected into lung cancer cell line A549 via FUGENE 6 liposome, ploidy, and cell cycle were studied by flow cytometry combined with fluorescent staining,and DNA index (DI) and SPF were calculated. 方法:将IRE-EGFP-esp1质粒用Fu-GENE6脂质体转染到肺腺癌细胞A549,经G418筛选获得转染阳性的细胞系(esp1-A549)。 采用DNA荧光染色流式细胞仪分析转染后细胞DNA含量、倍性及细胞周期,计算S期细胞比率(SPF)和DNA指数(DI);
