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- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 构建的真核表达载体pcDNA3 .;1(-) ASL经过酶切鉴定和测序证实正确。
- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题： 图1.;穿梭表达载体pRL_hEGF的构建。
- We have made up an eukaryotic (Pichia pastoris) expressive vector (pPic9k-DCN). After the linear recombinant vector was introduced into HIS~-/GS115 cells,we have selected positice clones against G418(4mg/ml)and confirmed by PCR. 构建了真核表达载体pPic9k-DCN，并将酶切线性化的重组载体转入酵母菌HIS~-/GS115后，筛选出了抗高浓度(4mg/ml)G418的阳性克隆，并用PCR法进行了鉴定。
- The fragment containing UBE gene was inserted into pGEX-4T-2 expressive vector, and induced by IPTG. Molecular weight of ubiquitin fusion protein was about 40 kD by checking with SDS polyacrylamide gel electrophoresis. 将烟夜蛾的UBE基因克隆到原核表达载体pGEX-4T-2上,经IPTG诱导,SDS-PAGE电泳检测出约40kD的融合蛋白。
- Methods An open reading frame of human BSP was amplified by PCR method and was reconstructed into the eukaryotic expressive vector pIRES2-EGFP to construct the hBSP sense or antisense expressive vector . 方法以PCR的方法扩增人BSP的开放阅读框序列,与载体pIRES2-EGFP相连构成重组正反义表达质粒。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- Primers were designed on the open reading frame of the cyclephilin gene of tea plant to construct the expressive vector pET/Csin-Cyp. A recombinant protein about 23 kD in the Escherichia coli BL21 (DE3) was induced. 根据亲环素基因开放阅读框序列设计引物,构建了原核表达载体pET/Csin-Cyp,并在大肠杆菌BL21(DE3)中成功诱导出了一个分子量为23kD的亲环素融合蛋白。
- Objective:To construct an eukaryotic expressing vector pIRES1neo/hFL and express hFL in COS 7 cell. 目的 :构建人FL真核表达载体 pIRES1neo/hFL ,观察其在COS 7细胞中的表达。
- The cDNA of BcpLH gene was cloned into a His-fusion expression vector pET-28a (+) and was induced to express in E. colt strain BL21(DE3). 在含有His标记序列的原核表达载体pET28-a(+)上插入Bc-pLH基因的cDNA,在大肠杆菌BL21(DE3)中诱导表达出了特异性蛋白,并免疫大白兔制备出高效价的抗血清;
- Objective To construct endothelial cell-specific molecular-1(ESM-1) eukaryotic express vector,transfect it into human umbilical vein endothelial cells( HUVECs,ECV304) and express it in vitro . 目的 构建人内皮细胞特异性分子 1(ESM 1)真核表达载体 ,转染人脐静脉内皮细胞系ECV30 4 ,并在ECV30 4中获得表达。
- Objective: To construct a procaryotic expression vector of vascularendothelial growth factor 165 (VEGF165) and to express VEGF165 inE. coli. 目的:构建人血管内皮生长因子 165(Vascular endothelial growthfactor 165,VEGF165)原核表达载体并在大肠杆菌中诱导表达。
- Objective:To construct and express a recombinant eukaryotic expression vector bearing fusion gene of human IL-2 cDNA gene and Fc fragment. 目的 :构建含人IL 2cDNA基因和免疫球蛋白Fc片段融合基因的真核表达载体pcDNA 3.;1IL 2 /Fc;并在真核细胞中表达;以期用于乙型肝炎病毒 (HBV)DNA疫苗的研究。
- AIM: To clone human IL-24 (hIL-24) gene and construct its eukaryotic expression vector, then transfect it into Ca Ski cells to express hIL-24 protein. 目的:克隆人IL-24基因并构建真核表达载体;转染Ca Ski细胞进行真核表达.
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Eukaryotic expression vector pCDNA3-AADC can be efficiently expressed in primary muscle cells. 含人类神经元AADC基因的真核表达重组体可在原代培养的骨骼肌细胞中有效的表达。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
