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- Fig.1.Construction of the shuttle expression vector pRL_hEGF. 标题： 图1.;穿梭表达载体pRL_hEGF的构建。
- The pPIC9K/Ang-1 yeast expression vector was constructed successfully. 成功构建pPIC9K/Ang-1毕氏酵母表达载体。
- Aim To construct a CHO cell expression vector carrying coamplification gene. 目的构建携带共扩增基因的CHO细胞表达载体。
- And then the CED-9 gene was inserted into plant expression vector pBI121 under the control of CaMV 35S promoter. 在此基础上再构建重组表达载体pBI-ced9，将CED-9基因置于CaMV35S启动子控制之下。
- The baculovirus expression vector pAC-HBs-Fc for complete IgG antibody against HBsAg was successfully constructed. 已成功构建表达载体pAC-HBs-Fc,为表达抗人HBsAg的IgG全抗体奠定了基础。
- The cDNA was subcloned into prokaryotic expression vector pET3d and overexpressed in E. coli BL21(DE3). 将该cDNA插入原核表达载体pET3d并在大肠杆菌BL21(DE3)中过量表达。
- Objective To construct the antisense expression vector of human Na + H + exchanger 1 (NHE 1). 目的 构建人Na+ H+ 交换蛋白 1(Na+ H+ exchanger 1,NHE 1)基因反义真核表达载体。
- Cloning of recombinant human BMP2 gene in eukaryotic expression vector provide basis for BMP2's expression. 克隆获得人骨形成蛋白 2基因 ,并得到此基因的真核表达载体 ,为人骨形成蛋白 2的表达打下了基础。
- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
- The recombinant constitutive expression vector pGAP9K-gat was constructed by inserting the aim gene into pGAP9K. PCR拼接获得250bp的目的基因序列,将目的基因克隆于pGAP9K,获得组成型表达载体pGAP9K-gat。
- The competent Agrobacterium tumefacien was transformed by rice expression vector p13W4-CTB and pCWUN respectively. 水稻表达载体P13w4一cTB和pcWIJN分别转化感受态根瘤土壤杆菌，经过PcR筛选和酶切鉴定，获得根瘤土壤杆菌转化子。
- For mass production of hEGF, Bombyx mori baculovirus expression vector system was adopted in this experiment. 为了高效表达人表皮生长因子，我们采用了家蚕杆状病毒表达系统(Bombyx mori baculovirus expression vector system, BMBEVS)。
- The BADH and CMO ORF were obtained and the plant expression vector pCU1303-BADH, pCU1303-CMO were constructed. 设计带特定酶切位点的特异引物,分别克隆了麦冬BADH和CMO的编码区片段,构建了植物表达载体pCU1303-BADH和pCU1303-CMO。
- Both genes were cloned into GFP expression vector pIRES2 EGFP and transfected into HeLa cells. 将上述基因克隆入绿色荧光蛋白(GFP)表达载体pIRES2-EGFP中,转染人HeLa细胞,在荧光显微镜和电镜下观察转染细胞的形态和结构。
- The ORF was inserted into expression vector pSE-380, then transformed into E. coli JM109 and E. 将此ORF连接入质粒pSE-380中，并在大肠杆菌JM109和BL21中表达。
- Conclusions 1. The expression vector pS2-DTA contains the EWS-FLI-1 binding sequence and the DTA gene sequence. 结论1、我们通过定向克隆的方法，成功地构建了含EWS-FLI-1特异结合序列和白喉毒素A链基因的真核表达载体。
- Construction of the success of this experiment CHH original expression vector, and given the inclusion of CHH. 本实验成功构建了CHH原核表达载体，并且获得了CHH包涵体蛋白。
- Conclusion: The prokaryotic expression vector with target gene was constructed successfully. 结论:成功构建了带有目的基因的原核表达载体。
- Kitts P A,Possee R D.A method for producing recombinant baculovirus expression vector at high frequency(J). 张志芳张颖吕鸿声等.;家蚕核多角体病毒解旋酶基因的克隆及部分序列分析（J）[J]
- AIM: To clone human soluble TRAIL cDNA and construct its eukaryotic expression vector to prepare a condition for targeted gene therapy. 克隆人可溶性trail(strail)基因并构建肿瘤细胞特异性真核表达载体;为肿瘤基因治疗的靶向性奠定实验基础.
