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- By analyses of the SDS-PAGE and Western blotting,it was showed that cGH protein was expressed in Pichia pastoris GS115. 对重组酵母进行诱导表达,SDS-PAGE和Western印迹分析,结果表明cGH在毕赤巴斯德酵母GS115菌株中获得了高效表达。
- The two-step fermentation conditions of opioid peptide expressed in Pichia pastoris were studied. 研究了由毕赤酵母胞内两步发酵法表达活性小分子阿片肽的条件。
- Objective To purify the hepatitis B virus(HBV) preS2(epi)S protein expressed in Pichia pastoris and study its immune effect. 目的研究重组毕赤酵母表达的乙型肝炎病毒preS2S蛋白的纯化方法及免疫效果。
- Conclusion Highly-active recombinant mutant human annexin V with endogenous metal-chelating sites can be expressed in Pichia Pastoris system. 结论 利用毕赤酵母系统表达出高活性的、具有内在金属螯合位点的突变型人膜联蛋白V。
- Cecropin-XJ expressed in Pichia pastoris was purified through ion exchange chromatobraphy(IEC),hydrophobic interaction chromatography(HIC) and size exclusion chromatography(SEC). 通过离子交换层析、疏水作用层析和分子排阻层析等三步层析的方法,分离纯化了毕赤酵母工程菌所表达的新疆家蚕抗菌肽。
- Cloning of mMR-1 Gene and Expression in Pichia pastoris Systems mMR-1基因的克隆和在毕赤酵母中分泌表达
- Con-clusions The rhES gene can be expressed in Pichia pastoris, and has the ability to restrain the growth of ECV-304 cells and lung adenocarcinoma Astc-a-1 in nude mice, showing important potentials for future clinical applications. 动物实验表明其能够有效抑制裸鼠肺腺癌Astc-a-1细胞的生长;统计学分析有非常显著性差异(P<0.;01)。 结论酵母系统内能够大量表达具有生物学活性的重组人内皮抑素;能在人体外有效抑制裸鼠肺腺癌Astc-a-1的生长;对其应用于临床具有重要意义。
- Cloning of a Laccase Gene from Neurospora crassa and It's Preliminary Expression in Pichia pastoris 粗糙脉孢菌漆酶基因的克隆及在毕赤酵母中的初步表达
- Modification of hybrid antimicrobial peptide CecA-mil gene and its over-secretion expression in Pichia pastoris 杂合抗菌肽CecA-mil的改造及在毕赤酵母中的分泌表达
- Random Mutations of Antibacterial Peptide DCD-1L and Expression in Pichia Pastoris 抗菌肽DCD-1L的随机突变及在毕赤酵母中的表达
- AIM: To express human anti-keratin Fab in Pichia pastoris secretively and optimize the expression condition. 目的:用巴氏毕赤酵母表达抗角蛋白抗体Fab段并优化表达条件。
- The successful expression of the anti-keratin Fab in Pichia pastoris has laid a solid foundation for its further application. 人源性抗角蛋白Fab段抗体在巴氏毕赤酵母菌中获得成功表达,为其进一步的应用研究打下了基础。
- Purpose To construct a recombinant plasmid pPIC9k rPA for the expression of whole length of rPA gene in Pichia pastoris. 目的构建含rPA全长基因的重组酵母胞内表达质粒pPIC9K rPA ,并在毕赤酵母中进行表达。
- expression in Pichia pastoris 酵母表达
- Cloning of Human Lysozyme Coden Gene and Expressing in Pichia Pastoris for Natural Food Antiseptic 天然食品防腐剂人溶菌酶编码基因的克隆及其在毕赤酵母中的表达
- Objective To study on the expression of His-Tagged S protein of hepatitis B virus in Pichia pastoris and its application in combination with the blood-origin hepatitis B virus Dane particles for immunoassay of HBsAb. 目的用DNA重组技术在毕赤酵母中表达多聚组氨酸融合重组乙型肝炎病毒表面抗原S蛋白,与血源乙型肝炎Dane颗粒联合使用,制备乙型肝炎表面抗体酶免试剂盒。
- Previous studies in our lab has generated an chimeric antigen named PfCP-2 which is consist of two Plasmodium falciparum erythrocytic stage antigens, AMA-1(III) and MSP1-19. Extremely high level expression of the gene was achieved in Pichia pastoris. 我们实验室已构建了由AMA-1(III)和MSP1-19组成的恶性疟原虫红内期融合抗原PfCP-2，并在毕氏酵母中得到了高水平表达。
- The stability of HIP gene in the recombinant Pichia pastoris was studied and the results showed that the recombinant plasmid had good stability in Pichia pastoris. 对毕赤酵母中外源基因的稳定性进行了研究;结果表明外源基因在毕赤酵母中很稳定.
- Many recombinant hydroxynitrile lyases have been expressed in Escherichia coli, Saccharoinyces cerevisiae and Pichia pastoris. 许多重组醇腈酶业已在大肠杆菌，啤酒酵母和甲醇酵母中成功表达。
