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- Results GUS assay analysis showed the expression of exogenous gene. 结果利用GUS染色法,确认外源质粒片段基本表达良好;
- In addition to encode many special characters,plasmid of bifidobacteria also can be used as tools to express various exogenous genes. 双歧杆菌质粒除了编码许多特殊性状外,还是在双歧杆菌中表达各种外源基因的重要工具。
- The development of transgenic technology provide a new method to breed new varieties of crops with exogenous genes, many transgenic crops resistant to pests have been bred. 摘要现代生物技术的发展为利用外源基因培育优良作物品种提供了新的途径，目前国内外已培育出多个高抗害虫的转基因作物。
- Results The engineering yeast strain didn't lose the exogenous gene after the subculture. 结果该酵母工程菌经多次传代后外源基因不丢失。
- It is feasible to use Agrobacterium-mediated method to introduce exogenous gene into Roselle cells via callus. 说明采用农杆菌介导法将外源基因经愈伤组织导入玫瑰茄细胞是可行的。
- The promoter promoting the exogenous gene's transcription in the plant recipient is the essential condition that the exogenous gene can express. 启动子驱使外源基因在受体植株中启动转录是外源基因能够表达的必要条件。
- The dispersed pancreas cells were transfected with exogenous gene by adenovirus vector and cationic polymer reagent, respectively. 在细胞分散后对孔鳐胰脏细胞用腺病毒载体和阳高子多聚物进行外源基因的转染。
- Distant cross is an effective method creating new specses,new germplasm and transfering exogenous gene in biological bodies. 远缘杂交是创造生物新物种和新种质以及转移遗传物质的有效途径,在理论和实践上都有重要意义。
- The recent advances about laser microbeam in plant transformation of exogenous gene, key techniques and applications are introduced. 本文概述了近年来激光微束在植物外源基因转化上的最新进展，激光微束转化法的关键技术及应用。
- Object iv e To study the possibility of prevention of posterior capsular opacification(PC O)by transfer of exogenous gene into lens epithelial cells. 目的观察目的基因在晶状体上皮细胞的表达情况,以探讨基因疗法用于后发性白内障的可行性。
- Expression of exogenous genes was detected by immunocytochemical staining methods at 72 hours after AdB2V infection by rabbit bone marrow stromal cells, and the uninfected group was taken as control. AdB2V感染兔骨髓基质干细胞后72h以免疫细胞化学染色方法检测外源基因的表达,以未感染组为对照。
- In this paper, the safety of GM food was introduced,the DNA degradation (especially transferred exogenous genes) during the processing of transgenic crops into food products was reviewed,and the future study was put forward. 文中阐述了转基因食品的安全性问题,评述了转基因食品DNA在加工过程中变化规律的研究进展,并提出了转基因食品内外源基因在加工过程中降解变化研究思路。
- Methods:The recombinant plasmid pIRApoptinHNIL18 was introduced into human colon carcinoma cells HCT-116 by liposome-mediated transfection. The expression of the exogenous genes was detected by Western blotting and RT-PCR. 方法:重组质粒pIRApoptinHNIL18通过脂质体介导法体外转染人结肠癌细胞HCT-116,通过Western blotting和RT-PCR检测外源基因表达;
- To inestigate the long-term culture conditions of bone marrow stromal cells , their efficacy of expressing exogenous gene and their ability to differentiate into adipocytes. 研究骨髓间充质细胞的体外长期培养下外源基因的表达和向脂肪细胞分化的能力。
- This review introduced the research process of the establishement of genetic transformation system in Maize. The method and integration and expression of exogenous genes were also discussed. 本文介绍了近年来玉米遗传转化系统的建立、基因导入手段、方法等方面的研究进展。
- Abstract Objective To explore the culture conditions for the neural stem cells from embryonic rat cortices and to test their efficacy of expressing exogenous gene. 摘要 目的 探讨胚胎大鼠神经干细胞的体外培养条件及外源基因的表达效率。
- The new germplasm-wheat with exogenous genes is the most valuable natural material for ?breeding of new variety.Identifying of exogenous genes rapidly and correctly is foundation to the breeding. 具有外源基因的小麦新种质是极其宝贵的育种新材料，快速、准确地鉴定小麦中的外源基因，有利于加速其在育种上的利用。
- The recent advances about laser microbeam in plant transformation of exogenous gene, key techniques and applications are introduced. And the prospects is anticipated. 摘要本文概述了近年来激光微束在植物外源基因转化上的最新进展，激光微束转化法的关键技术及应用。并对其今后的应用前景作了展望。
- Objective To obtain the gene encoding tomato fruit-specific E8 promoter therefore to prepare for exogenous gene transcription and expression in transgenic tomato fruit. 目的获取番茄果实特异性E8启动子基因,为实现外源基因在转基因番茄中果实特异性表达做准备。
- Expression of D1x5 mRNA disapears at the fourth passage NSCs,but can be recovered by exogenous gene transfect ion and treatment with BMP-2. In the meantime, D1x5 gene can direct in vitro expanded SVZa NSCs differentiating into neurons. 随着传代次数的增加,到第4代时,SVZa NSCs D1x5 mRNA表达消失,而外源性D1x5 基因转染及BMP-2可以诱导其重新表达; D1x5基因能使新生大鼠来源的SVZa NSCs向神经元方向分化。
