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- Amplified of the green fluent protein (GFP) gene from plasmid PCR3.1, and add suitable restriction enzyme cutting site. 从含有gfp基因的质粒pCR3.;1上扩增出全长基因，并且加上合适的酶切位点。
- The expressive vector pcDNA3.1(-)-ASL has been constructed and had been confirmed by restriction enzyme cut and sequencing. 构建的真核表达载体pcDNA3 .;1(-) ASL经过酶切鉴定和测序证实正确。
- The DNA fingerprint atlas of Angora goat, Jianchang black goat,and their F 2,F 3 generations have been established by using(CA/GATA/TCC) 5 as a probe and Hinfi enzyme cutting. 以 (CA/GATA/TCC) 5探针 ;HinfI酶切 ;研究安哥拉山羊与建昌黑山羊杂交的亲本及其F2 、F3 的DNA指纹图谱 .
- About 600bp DNA (p-CpG-DNA) which contained 62 CpG motifs was obtained by the method of plasmid recombination and PCR. CpG-DNA2 composed of two CpG-ODN monomers was prepared by enzyme cutting P-CpG-DNA. 采用质粒重组和PCR方法，将其串联成长为600多碱基对的DNA(P-CpG-DNA)，其中含有62个CpG基序，并将其中一部分用Sall酶切得到含两个CpG-DNA单体的CpG-DNA2。
- NS genes of muscovy duck reovirus S14 and C4 were amplified by RT_PCR with the primers designed by the reported avian and muscovy duck reoviruses. The PCR products were cloned to the pMD18_T vector and identified by enzyme cutting analysis. 参考GenBank禽呼肠孤病毒 (AvianReovirus,ARV)和番鸭呼肠孤病毒 (MuscovyDuckReovirus,MDRV)非结构基因 (NS)序列设计合成一对引物 ,对番鸭呼肠孤病毒S14和C4株NS基因进行RT_PCR扩增 ,克隆到pMD18_T载体中 ,并对克隆产物进行酶切鉴定和测序 ;
- Enzymes cutting confirmed the positive directed ligation between AADC gene and pCDNA3. The recombinants were expressed successfully in primary muscle cells. 酶切证实基因片断正向连接于pCDNA3上,并在原代骨骼肌细胞中获得表达。
- Rose bushes shoot again after being cut back. 玫瑰丛修剪後还能再长出新枝。
- In order not to be late, she cut across the fields. 为了不迟到，她抄近路穿过田野。
- He cut off a bunch of grapes to entertain us. 他剪下一串葡萄招待我们。
- She reckoned she had cut her cost by half. 她估计她减少了一半的费用。
- The flood cut a wide swath in this area. 洪水席卷了这一地区，满目一片疮痍。
- The slide cut off the valley from the rest of the world. 山崩切断了这个山谷与外界的联系。
- I must say I didn't like the cut of his jib. 说实在的，我不喜欢他那个样子。
- I cut myself on the cheek with my razor. 我不小心被刮胡刀割伤了脸颊。
- Peter and Susan seem to be cut out for each other. 彼得和苏珊似乎是天生的一对。
- The comedy tine was cut out as being off side. 笑料由于低级趣味而被删去。
- She was cut to the quick by his insults. 她受他侮辱而十分伤心。
- Our tanks cut in ahead of the escaping aggressors. 我们的坦克抢到前面去拦住那些溃逃的侵略者。
- He often cut recitation during his college days. 他上大学时经常无故旷课，逃避背诵练习。
