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- In trial experiment endometrial lymphocyte(EML) from pregnant rabbit were obtained by digestion with trypsin and then by decenfugation. 预实验采用酶消化及密度梯度离心法分离妊娠家兔EML,并采用噻唑蓝(MTT)法进行体外活化试验。
- Methods: Larvae of Trichinella spiralis isolated from the muscle of a Trichinella - infected mouse were put in a glass homogenizer for homogenizing, digesting with trypsin. 方法:将从小鼠肌肉分离、纯化的旋毛虫幼虫,放入玻璃匀浆器匀浆,胰酶消化。台盼蓝和中性红染色检测分离细胞的活性。
- digestion with trypsin 胰蛋白酶酶解
- This charge is reduced by digestion with neuraminidase. 这一电荷被唾液酸苷酶消化而降低。
- Methods Cells collected from porcine fetus pancreas were digested with trypsine and cultured in medium RPMI1640 with 10% FBS. 方法 无菌采集胎猪胰腺,经胰酶消化,以含10%25血清的RPMI1640培养基培养。
- Biological characteristics of Bt Cry proteinThe solubility, digestion of Cry protein with trypsin and growth speed of E. Bt Cry蛋白特性的研究
- Perfusing the infrarenal abdominal aorta with trypsin developed canine AAA model. 用胰蛋白酶灌注犬肾下腹主动脉段形成动脉瘤模型;
- In this paper,the enzymatic degradation of succinylated gelatin with trypsin was studied. 对以胰蛋白酶为催化剂,琥珀明胶为底物的酶解反应体系进行了考察。
- Methods HPMC was separated with trypsin and the synthesis of DNA was measured by 3H-TdR incorporation. 方法采用胰蛋白酶消化法,从人腹膜组织中分离间皮细胞,并建立体外人腹膜间皮细胞(HPMC)培养模型;
- The sample was digested with conc. 试样用浓硝酸及过氧化氢消解。
- Methods: The cerebral cortex of newborn rats was digested with trypsin to obtain single cell viability was estimated by trypan blue staining. the cells were planted; 方法:新生大鼠大脑皮层通过胰酶消化获取单细胞悬液,用台盼蓝染色鉴定活性后种板培养;
- Epidermis was separated from dermis,cut into pieces, digested with 0.25% trypsin for about 5-7 mins. 分离表皮与真皮;将表皮剪成糜状;0.;25%25胰酶37℃消化5-7 min后中和;过滤;离心;接种。
- Genomic DNA was digested with EcoRI combined with either MseI or TaqI. 35天后,小鼠晶状体核区完全白色混浊所覆盖,为核性白内障。
- Alice then encrypts the message digest with her private key to create her personal signature. 然后,小红用她的私钥加密该消息摘要以创建她的个人签名。
- When the clones growed confluently 85% or above, they was digested with 0.25% trypsin and treated by 3-MC or TCDD. 待细胞生长汇合度达到85%25以上,消化细胞,用3-MC或TCDD诱导筛选克隆。
- By using the method of culturing peripheral blood lymphocyte and obtaining with Trypsin and Giemsa,G-band of Xianju chickens were investigated. 采用外周血淋巴细胞培养和胰酶处理法,对仙居鸡染色体G带带型进行了研究。
- S-protein was isolated from RNase A by polyacrylamide gel electrophoresis afterproteolytic digestion with subtilisin. 核糖核酸酶A具有DNA结合蛋白的作用。
- The product of chondroitin sulfate was gotten after ethanol disposition and desiccation by eliminating the protein with trypsin and pepsin. 以胰蛋白酶、胃蛋白酶分解去除杂蛋白,经酒精沉淀并干燥后得到产品。
- The binding affinities of a series of benzamide inhibitors with trypsin were evaluated by molecular dynamics (MD) simulations with a linear response approach. 本文采用基于线性响应近似的自由能预测方法计算了胰蛋白酶和苯酰氨类抑制剂的结合自由能。
