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- Five degenerated primers were designed according to the P-loop, Kinase-2a, Kinase-r3a, EGF domain of NBS which universally exist in cloned R genes. PCR amplifications were performed with the designed RGA primers and cDNA as templates. 根据已克隆R基因普遍具有的NBS结构域的P-loop、Kinase-2a、Kinase-3a、EGF区设计5条RGA简并引物，组成5对引物组合。
- Methods The erm genes of MRCNS were detected by degenerate primers amplification. 方法 应用兼并引物扩增MRCNS菌的erm基因。
- The SOX genes of Panthera tigris altaica Temminck were detected by a pair of degenerate primers. 应用特异于HMG bOX区域的兼并引物 ,扩增了东北虎的SOX基因。
- Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. 分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。
- In This study, a set of degenerate primers were designed according to the conserved amino acid sequences of CYP6A1, CYP6DI in Musca domestia and CYP6E1 in Culex quinquefasciatus. 根据家蝇CYP6A1、CYP6D1以及致倦库蚊CYP6E1的保守区氨基酸序列设计一组简并引物,采用RT-PCR的方法,从白纹伊蚊四龄期活体幼虫总RNA中扩增出与设计相符的基因片段。
- Then , partial eDNA of Luffin ~2 was cloned by RT-PCR with two primers ( one was degenerate primer for 5?terminal primerand the other was a general primer containing oligo-dT). N－端 9个氨基酸的序列，并据此设计5，端简井引物，用此引物和 3’－端通用引物，通过RTFCR技术克隆了 Luffin S。 的部分cDNA序列。
- SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes. SOX基因兼并引物延伸发现了更多的SOX基因位座，并进一步证实该家族基因在基因组中是散在存在的。
- The method that finds Resistance Gene Analogs (RGA) using the degenerate primers designed based on the conserved motifs of plant disease resistance genes (R) was named as RGA method. 植物抗病基因(Resistance Gene,R)产物存在保守结构域,如NBS、LRR、STK、TIR、TM和LZ等。 根据这些保守序列设计简并引物寻找R基因同源序列(Resistance Gene Analogs,RGA)的方法称为RGA法。
- According to the homology of 6 kinds of amino acid accession sequences of antifungal peptide Drosomycin from Drosophila melanogaster in GenBank, the degenerate primers(Drs1/Drs2) were designed. 根据果蝇中发现的抗真菌肽Drosomycin因子在GenBank中的6个登录序列，按其同源区段的氨基酸序列设计简并引物Drs1和Drs2，以生活在潮湿腐生环境中的9种拟靶昆虫为研究对象，通过PCR方法，希望在基因组水平上搜查新的昆虫抗真菌肽拟靶基因序列。
- He ate up Nietzsche and degenerated into a fascist. 他接受了尼采的思想，堕落成法西斯分子。
- Children learn knowledge from primers. 孩子们从启蒙书上学习知识。
- Keywords Bacillus fastidious uricase;degenerated primer PCR;kinetic uricase method for serum uric acid assay;xanthine interference;gene cloning; 关键词苛求芽孢杆菌尿酸酶;血清尿酸测定专利尿酸酶法;黄嘌呤干扰;简并引物PCR;基因克隆;
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- He has degenerated into an alcoholic. 他堕落成酗酒者。
- degenerated primers 简并性引物
- All animals degenerated in America. 一切动物到了美洲就要退化。
- The argument degenerated into a row. 争论变成吵架。
- Her commitment to a great cause degenerated from a crusade into an obsession. 她致力于一伟大事业,但其崇高的奋斗精神已变质成为偏执的狂热。
- South family store has a 'Degen'. 家乐南楼店有个‘德根’。
- I am the sun: So like carbon black Degen. 我很阳光：所以黑得跟炭似的。
