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- Methods The erm genes of MRCNS were detected by degenerate primers amplification. 方法 应用兼并引物扩增MRCNS菌的erm基因。
- The SOX genes of Panthera tigris altaica Temminck were detected by a pair of degenerate primers. 应用特异于HMG bOX区域的兼并引物 ,扩增了东北虎的SOX基因。
- Here we present the results of random primer and SOX degenerate primer PRINS on human metaphase chromosomes. 分别应用随机引物和SOX基因兼并引物在人类染色体上进行了原位延伸标记。
- In This study, a set of degenerate primers were designed according to the conserved amino acid sequences of CYP6A1, CYP6DI in Musca domestia and CYP6E1 in Culex quinquefasciatus. 根据家蝇CYP6A1、CYP6D1以及致倦库蚊CYP6E1的保守区氨基酸序列设计一组简并引物,采用RT-PCR的方法,从白纹伊蚊四龄期活体幼虫总RNA中扩增出与设计相符的基因片段。
- Then , partial eDNA of Luffin ~2 was cloned by RT-PCR with two primers ( one was degenerate primer for 5?terminal primerand the other was a general primer containing oligo-dT). N－端 9个氨基酸的序列，并据此设计5，端简井引物，用此引物和 3’－端通用引物，通过RTFCR技术克隆了 Luffin S。 的部分cDNA序列。
- SOX degenerate primer PRINS revealed more SOX gene loci and proved further that SOX genes were not clustered on human chromosomes. SOX基因兼并引物延伸发现了更多的SOX基因位座，并进一步证实该家族基因在基因组中是散在存在的。
- The method that finds Resistance Gene Analogs (RGA) using the degenerate primers designed based on the conserved motifs of plant disease resistance genes (R) was named as RGA method. 植物抗病基因(Resistance Gene,R)产物存在保守结构域,如NBS、LRR、STK、TIR、TM和LZ等。 根据这些保守序列设计简并引物寻找R基因同源序列(Resistance Gene Analogs,RGA)的方法称为RGA法。
- According to the homology of 6 kinds of amino acid accession sequences of antifungal peptide Drosomycin from Drosophila melanogaster in GenBank, the degenerate primers(Drs1/Drs2) were designed. 根据果蝇中发现的抗真菌肽Drosomycin因子在GenBank中的6个登录序列，按其同源区段的氨基酸序列设计简并引物Drs1和Drs2，以生活在潮湿腐生环境中的9种拟靶昆虫为研究对象，通过PCR方法，希望在基因组水平上搜查新的昆虫抗真菌肽拟靶基因序列。
- Five degenerated primers were designed according to the P-loop, Kinase-2a, Kinase-r3a, EGF domain of NBS which universally exist in cloned R genes. PCR amplifications were performed with the designed RGA primers and cDNA as templates. 根据已克隆R基因普遍具有的NBS结构域的P-loop、Kinase-2a、Kinase-3a、EGF区设计5条RGA简并引物，组成5对引物组合。
- Children learn knowledge from primers. 孩子们从启蒙书上学习知识。
- Arbitrary are summarized. The application and perspective of degenerate primer 简并引物
- This degenerate seduced my daughter! 这个道德败坏的人勾引我的女儿！
- I borrowed from the library a primer of phonetics. 我从图书馆借了一本语音学入门书。
- Application of degenerate primers to amplify fragment of pheromone receptor gene in Auricularia auricula 应用简并引物扩增黑木耳信息素受体基因片段
- Identification of New EST and Genes with Zinc Finger Motif by RT-PCR Using Degenerate Primers 应用简并引物RT-PCR扩增锌指结构域筛选新基因
- Keywords Integrons;gene cassette;degenerate primers;Drugresistance;PCR;polymorphism;RFLP Sequenceanalysis;outer membrane protein; 整合子;基因盒;简并引物;耐药;聚合酶链反应;多态性限制性片段长度;外膜蛋白;
- He didn't let riches and luxury make him degenerate. 他不因财富和奢华而自甘堕落。
- South family store has a 'Degen'. 家乐南楼店有个‘德根’。
- I am the sun: So like carbon black Degen. 我很阳光：所以黑得跟炭似的。
- Keywords ABA binding protein,degenerate primer,RACE,Pichia methanolica expression system,affinity chromatography; 脱落酸结合蛋白;简并引物;RACE;酵母表达系统;亲和层析;
