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- Objective:To construct expression vector of human ID4 gene promoter on the basis of bioinformatics analysis. 目的:基于生物信息学分析构建人ID4基因启动子表达载体,以其作为研究ID4基因启动子表达调控分析的工作基础。
- Optimized-code bull protamine gene was synthesized, the code (CGT) which E. coli. is partial to was substituted for the rare codes(AGA or AGG), the gene was cloned into pGEX-2T to construct expression vector pGEX-2T-PS. 利用大肠杆菌偏好的编码精氨酸的密码子CGT将野生型牛精蛋白基因中编码精氨酸的稀有密码子 (AGA或AGG)替换掉 ,通过基因合成得到密码子优化的牛精蛋白基因 ,将其克隆入原核表达载体pGEX 2T ,组装成表达载体pGEX 2T PS。
- The main methods and results are as follows: 1.The constructed expression vector, pMSCV-ncam, was constructed and proved correct by enzyme restriction pattern. 2. 实验方法结果如下： 1.;对已构建含有NCAM的质粒载体进行酶切鉴定，证明表达载体构建正确，转化入细菌(TOP10)，扩增，纯化，备用。
- An expression of pain flitted across her face. 她脸上闪过一种痛苦的表情。
- He always shows a doleful expression. 他总是露出忧郁的表情。
- I can think of no greater expression of defeatism. 这是我听到的最悲观的失败主义论调了。
- A constant, variable, function name, or expression. 一种常数、变量、函数名或表达式。
- She looked at me without expression. 她毫无表情地朝我看着。
- The scenery there was beautiful beyond expression. 那儿的风景美丽得无法形容。
- He put forward a constructive suggestion. 他提出了一个建设性的建议。
- He looked at her with a solemn expression. 他表情严肃地望着她。
- There was an expression of discontent on her face. 她脸上有不满的表情。
- It took them two years to construct the bridge. 他们用了两年时间建这座桥。
- People have the rights of assembly and expression. 人们有集会和发表言论的权利。
- She gave expression to her sadness. 她流露出了悲伤之情。
- The expression or manifestation of such feeling. 欢欣，喜悦，高兴的表情或表现
- The level of formality determine the precise mode of expression. 不同的礼节程度决定所需的确切表达方式。
- "To pull a face" is an idiomatic expression. "To pull a face""愁眉苦脸"是一个习惯表达法。
- He had a passive expression on his face. 他脸上有一种漠然的表情。
- He looked at me with an incredulous expression. 他怀疑地看着我。
