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- This study mainly deals with cell transfection and cytotoxicity for PEI(10kD)-PBLG, a novel cationic copolymer, to observe its potential as a gene carrier. 对新型阳离子聚合物PEI(10kD)-PBLG进行研究,重点考察其基因转染效率与细胞毒性,探讨其作为基因载体的可能性。
- Immunohistochemical staining showed that after mouse NIH3T3 cell transfection for 4 days positive expressive products appeared in cytoplasm, and control group showed negative result. 免疫组织化学染色显示:小鼠NIH3T3细胞转染4d后胞浆内可观察到阳性表达产物,对照组呈阴性结果。
- Calcium Phosphate Cell Transfection Kit 碧云天生产的磷酸钙法细胞转染试剂盒
- ASPC-1 cell transfected with the plasmid pEAKS contained PTEN was preferentially killed through apoptosis under hypoxia conditions. ASPC一1细胞缺氧后PI3K传导通路的激活起主要作用，常氧及缺氧下接受4仰单次照射未见ASPC一1细胞细胞周期产生明显的改变，同时应用LY294002、PD98O59S缺氧后照射引起的细胞凋亡明显高于其它各组。
- The growth of cell transfected with PTEN was dramatically ~inhibited compared with the parent ASPC cell or ASPC-1-pEAK8 cell. ASPC1pEAK8PTEN细胞生长曲线平缓,生长速度较另两种细胞明显降低。
- The transfected Balm cell showed apoptotic changes such as condense of nucleus and dark staining,apoptotic peak under FACS examination compared with the Balm cell transfected with only pcDNA3 vector. 发现转导后细胞生长代谢受抑，细胞体积缩小，有细胞碎块脱落，FACS检测出现典型凋亡峰；
- A storage cell in some amorphous memory devices. 某些非晶体存储器中的一个存储单元。
- We transfect the recombinant MEK gene into human umbilic vein endothelial cells ECV304, then examine the change of ESM-1 expression in cells transfected with recombinant MEK gene of differenceactivity . 用重组MEK基因转染人脐静脉内皮细胞ECV304，加以鉴定，并检测转染细胞中ESM-1表达的改变。
- She was found prostrate on the floor of the cell. 有人发现她趴在小屋的地板上。
- The COS-7 cells transfected with pEGFP-N3-GAP-43 expressed GAP-43 protein efficiently. 所构建的GAP-43质粒在COS-7细胞中获得高效表达。
- The prisoner rapped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- Transfection, make stable C/EBP p expression cell line. 质粒转染，制备稳定表达C尼BPp的细胞系。
- Results COS?7 cells transfected with constructed PCIneo?hTPO expressed and secreted hTPO up to 48.28 ng/ml in the supernatant. 应用hTPOcDNA转染的COS－7细胞表达产物对实验性小鼠血小板减少症进行腹腔注射。 结果hTPOcDNA不仅可在COS－7细胞中获得表达，且分泌到细胞培养上清中，最高表达量为48.;28ng/ml。
- The prisoner raped out a message on the cell wall. 那囚犯敲打囚室的墙来传达讯息。
- The growth of successive layers of a cell wall. 敷着，附着细胞壁连续层的生长
- Cell density was associated with PEI/DNA transfection efficiency. 细胞密度影响转染效率;
- A 220 bp segment encoding rmBD2 was amplified by RT-PCR and proved by sequencing. The SiHa/K cells transfected pcDNA3.1(+) and SiHa had no expression of rmBD2 protein. 提取稳定转染4、6、8、10周SiHa/rmBD2细胞总RNA,RT-PCR反应扩增得到220bp左右的目的片段,而SiHa/K及SiHa细胞未见目的蛋白表达。
- The prisoner was locked in a cell. 那囚犯被关在单人牢房内。
- The prisoner paced the floor of his cell. 那犯人在牢房里来回地走著。
- The stage was designed to imitate a prison cell. 舞台设计成牢房的样子。
