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- Keywords electroporation;cell cultured in suspension;gene transfection;optimization; 电穿孔;悬浮细胞;基因转染;优化;
- cell cultured in suspension 悬浮细胞
- To improve the method of myoblast cell culture in vitro. 改进体外成肌细胞培养方法。
- Studies on Increasing Ginkgolides Synthesized in Suspension Cell Culture of Ginkgo biloba L. 提高银杏悬浮培养细胞内酯合成的研究。
- Method MTT colorimetric assay was used to measure the growth of MKN45 cell cultured in RPMI 1640 medium with and without 10% FBS, respectively. 方法 采用MTT比色分析法分别测定OCT对生长于无血清培养基及含 1 0%25胎牛血清 (FBS)培养基中MKN45细胞的调控作用。
- Methods Cell morphology,MTT assay and flow cytometry were used to examine in leukemia K562 cell cultured in low concentration serum and free serum. 方法应用形态学、MTT分析法、流式细胞术对在低血清以及无血清条件下的白血病细胞进行检测和观察。
- Advances in studies on tissue and cell culture in medicinal plants of Panax L. 人参属药用植物组织和细胞培养的研究进展。
- Methods The CHO/dhfr- cells were transfected with linearized plasmids of pMM-CTLA-4-IgG4/WG and pMMGR. The clones of CHO/pCTLA-4+/pMMGR+ were in suspension cultured in serum-free culture media. 方法将线性化的pMM-CTLA-4-IgG4/WG和pMMGR转染CHO/DG44细胞,对阳性克隆进行无血清悬浮培养,Western blot检测细胞培养液中CTLA-4/Ig融合蛋白的表达,筛选出高效稳定表达CTLA-4/Ig融合蛋白的CHO细胞;
- After 10 days,we found that there was higher level of IL-2 in the superantant of the peritoneal cell cultured in vitro, The macrophage cytotoxicity,splenocyte proliferation,NK activity,LAK activity were all augmented significantly after i.p. 同时经脂质体转染IL－2基因的脾脏细胞具有较高的内源性LAK活性。
- The bone defect of experimental side was filled with surface-demineralized bone allograft and bone marrow stem cell cultured in vitro.The control side was filled with bone allograft only. 将表面脱钙的同种异体骨和来源于受体的体外培养增殖的骨髓间质干细胞混合植入实验侧骨缺损,对照侧仅植入同样制作的异体骨。
- Methods: Cell culture in serum-free medium, indirect immunofluorescence cytochemistry were used. 方法：采用无血清细胞培养技术，间接免疫细胞化学染色法。
- My mind is in suspension until we win the war. 要等我们打赢了这场战争，我的思想才有着落。
- The metabolism efficiency and cell beating intension of cardiac cells cultured in HARV bioreactor was higher than those cultured in Spinner-flask bioreactors. 心肌细胞在两种不同培养体系中的细胞接种效率和细胞形态没有明显差异 ,培养于HARV中的心肌细胞的代谢效率和细胞搏动强度均明显高于Spinner flask培养体系。
- To be balanced or held in suspension;hover. 平衡；悬着在悬挂中处于平衡状态或被支持；徘徊
- This jewelry is made from epithelial skin cells cultured in a lab and grown in a test tube. 这些珠宝是用人体上皮细胞制成，又放在试管中培育出来的。
- To be balanced or held in suspension; hover. 平衡;悬着在悬挂中处于平衡状态或被支持;
- Gossypol and misopr0stol could directly damaged the luteal and decidual cells cultured in vitro. 醋酸棉酚和米索前列醇对体外培养大鼠黄体细胞和人蜕膜细胞都有明显的损伤作用，呈现量-效相关性。
- Both CGT expression and GalC expression were increased significantly in Schwann cells cultured in high glucose. 高葡萄糖时施万细胞CGT和GalC表达上调,可能影响施万细胞的活力。
- Also to be deeloped is the cell culture in three dimensions, as well as ensuring the biocompatibility of the material. 另外也需要发展立体细胞培养以及确保材料的生物相容性。
- Don't keep us in suspense any longer! 别再让我们着急了！
