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- To improve the method of myoblast cell culture in vitro. 改进体外成肌细胞培养方法。
- Methods: By techniques of cell culture in vitro, B Lymphomawere treated by RTE in 500ug/ml-4000ug/ml concentrations for24hr. 48hr. 72hr. 方法：应用体外细胞培养技术，以500ug/ml-4000ug/ml附子提取物作用于体外培养的B淋巴瘤细胞，同时设空白组，1.;0umol/L AS_2O_3阳性对照组，共同温育24hr、48hr、72hr、96hr后，在光镜、电镜下观察细胞形态学变化。
- Cell culture in vitro,ABC ELISA,RNA dot blot and atom absorption spectrum analysis were used to study biological effects of low concentration of iron citrate (Fe cit) on the expression of transferrin receptor (TfR) in healthy human peripheral lymphocytes. 为了研究铁缺乏的生物学作用，采用体外细胞培养、ABC－ELISA法、RNA斑点杂交和原子吸收光谱分析等技术方法，观察了低浓度柠檬酸铁（Fe－cit）对健康人外周血淋巴细胞转铁蛋白受体（TfR）及其基因表达的影响。
- Methods By techniques of cell culture in vitro, annexin-V-FITC and propidium iodide(PI)double staining and flow-cytometry (FCM), RBE cell line was treated by paclitaxel or together with MEK inhibitor PD98059 for 24 h. 方法运用体外细胞培养 ,AnnexinV 异硫氰酸荧光素和碘化丙啶双染法及流式细胞技术观察紫杉醇以及与ERK上游激酶MEK抑制剂PD980 5 9合用 2 4h后RBE细胞的凋亡情况 ,运用蛋白质印迹法观察紫杉醇以及与PD980 5 9合用后RBE细胞的ERK1,ERK2和ERK的活化形式 (pERK)的表达情况。
- Objective To study the biologic characteristics of leukemia stem cells(LSC) by primary leukemia cells culture in vitro. 目的通过对白血病原代细胞的体外培养,研究白血病干细胞(LSC)的生物学特性。
- Methods To observe the protecfive effecf of TF by measuring MTT metablism and cell nambers and cell culturing in vitro. 方法利用体外细胞培养技术，通过MTT测定、细胞计数等方法。
- Cell culture in vitro at high altitude 高原现场体外动物细胞培养的实验研究
- Cell culture in vitro of submandibular glands duct 颌下腺导管细胞体外培养的实验研究
- Methods:By technique of cell culture in vitro,Hela cells were treated with cisplatin in different concentrations for 72 hours,then apoptotic rate and cell cycle were measured by flow cytometer. 方法:运用体外细胞培养技术,以不同浓度的顺铂作用于培养的人Hela细胞72 h,用流式细胞仪分析其凋亡率及细胞周期。
- After 10 days,we found that there was higher level of IL-2 in the superantant of the peritoneal cell cultured in vitro, The macrophage cytotoxicity,splenocyte proliferation,NK activity,LAK activity were all augmented significantly after i.p. 同时经脂质体转染IL－2基因的脾脏细胞具有较高的内源性LAK活性。
- Method:Purified AFP from human cord blood serum was used to act on the hepatoma Bel7402 cells culture in vitro, and the concentration changes of Ca2+ were observed by confocal microscopic scanning. 方法:采用由脐带血血清中提纯的AFP作用于体外培养的人肝癌Bel7402细胞,通过共聚焦显微镜扫描,观察不同时间细胞内Ca2+浓度的变化。
- On hybrid embryo culture in vitro of Syringa L. 丁香(Syringa L.;)种间杂交幼胚离体培养研究
- Gossypol and misopr0stol could directly damaged the luteal and decidual cells cultured in vitro. 醋酸棉酚和米索前列醇对体外培养大鼠黄体细胞和人蜕膜细胞都有明显的损伤作用，呈现量-效相关性。
- Objective To observe the ultrastructural characteristics of human retinal progenitor cells cultured in vitro. 目的观察体外培养的人视网膜前体细胞的超微结构特征。
- A study of sensory and motor Schwann cell culture in vitro 感觉性和运动性神经膜细胞的培养研究
- The experiment of cryopreservation and cold storage of fibroblast cells cultured in vitro of Boer goat and yellow cattle's ear skin was conducted. 以体外培养的布尔山羊和黄牛耳组织成纤维细胞为研究对象 ,进行了哺乳动物体细胞的冻存与冷藏试验。
- Gao QW,Yang LG,Liang LM,et al.Study on rat bone marrow stromal cells cultured in vitro and osteogenic property.Chin J Aesthet Med,2005,14:281-283. [5]高全文;杨连甲;梁立民;等.;大鼠骨髓基质细胞体外培养诱导成骨能力的研究
- Establishment of Method for Human Endometrial Cell Culture in Vitro 人子宫内膜细胞原代体外培养方法
- Stem Segment Culture in Vitro of Idesia polycarpa Maxim. 山桐子茎段离体培养技术研究。
- AIM: To observe the expression of recombinated adeno-associated virus (rAAV) 1/2-lacZ vector gene in umbilical mesenchymal stem cells cultured in vitro. 目的:观察由重组腺相关病毒1/2载体携带的LacZ报告基因在体外培养的脐血间质干细胞中的表达情况。
