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Prame mRNA was revealed in 12 of 23 AML(67%),10 of 15 ALL (52%),5 of 9 CML patients in blast crisis,1 of 8 MM. 急性髓细胞性白血病(AML)23例中12例(52%25)，急性淋巴细胞性白血病(ALL)15例中10例(67%25)，CML9例急性变中5例Prame mRNA表达阳性。
The WT1 expression level of chronic myeloid leukemia(CML) in blast crisis was higher than that of CML in chronic phase. 慢性髓性白血病(CML)急变期患者WT1基因表达水平显著高于慢性期。
Objective To characterize the immunophenotype and cytogenetics ofchronic myeloid leukemia blast crisis (CML-BC). 目的研究慢性髓细胞白血病急变期(CML-BC)的免疫表型特征及细胞遗传学特征。
WT1 mRNA expression was revealed in K562 cell line, 10 of 12 AML patients, 2 of 3 ALL patients, 6 CML patients in blast crisis. K562 细胞株高度表达 W T1 ，急性髓性白血病（ A M L）12 例中10 例，急性淋巴细胞白血病（ A L L）3 例中2 例，慢性粒细胞白血病（ C M L）急性变6 例表达阳性；
A patient with T-LBL/ALL from extramedullary blast crisis of CML was verified by fluorescence in situ hybridization(FISH) analysis. 采用荧光原位杂交(FISH)方法确诊1例髓外发生T-LBL/ALL急变的CML患者。
T-LBL/ ALL from blast crisis of CML is a rare disorder,FISH analysis is essential for its diagnosis and differential diagno- sis. CML向T-LBL/ALL急变是一种罕见的疾病,FISH技术在本病的诊断和鉴别诊断中起关键作用。
Methods : We have chosen 37 CML cases, including 27 chronic phase (CP), 5 accelerated phase (AP) and 5 blast crisis (BC) cases . 方法:选取37例CML病人骨髓及口腔黏膜细胞的DNA标本,其中慢性期(chronic phase,CP)27例,加速期(accelerated phase,AP)5例,急变期(blast crisis,BC)5例。
The pre-BMT situations were, 91 cases in first chronic phase (CP1), 19 in accelerating phase (AP), 2 in blast crisis (BC) and 6 in advanced CP (>CP2). 118例患者中慢性期91例，加速期19例，急变期2例，第2次以上慢性期6例。
The WT1 positive rate in 15 CML patients was 46.7%, the positive rate in 6 patients at blast crisis and 1 patient at accelerated phase were 100%, 0 in 8 patients at chronic phase. 15例CML的WT1基因表达阳性率为46.;7%25，其中6例急变期和1例加速期阳性率为100%25，8例慢性期阳性率为0。
CML typically starts with an indolent chronic phase followed by an aggressive myeloid or lymphoid blast crisis phase, which is dependent on the constitutive kinase activity of the BCR-ABL oncoprotein. 依赖于BCR-ABL融和基因表达的激酶活性，CML患者通常有一个稳定的慢性期随后进入髓变或淋变的加速期。
WT1 mRNA expression was revealed in K562,HL?60 and TF?1 cell lines, 21 of 25 (84%) AML patients,8 of 11 (73%) ALL patients,2 CML patients in blast crisis and 1 of 7 CML patients in accelerated and chronic phase. K562、HL－60及TF－1三株白血病细胞系均高度表达WT1，而U937细胞系未测到WT1。急性髓系白血病（AML）25例中21例、急性淋巴细胞白血病（ALL）11例中8例、慢性粒细胞白血病（CML）急性变者2例、慢性及加速期CML患者7例中1例WT1表达阳性。
Objective To introduce the characteristics of T lymphoblastic lymphoma/acute lymphoblastic leukemia(T-LBL/ALL)from blast crisis of chronic myelogenous leukemia(CML),a case report and review of lite- rature. 目的介绍1例慢性髓细胞白血病(CML)向T淋巴细胞淋巴瘤/白血病(T-LBL/ALL)急变病例并复习文献,以提高对该病的认识。
In 39 CML patients, WT1 mRNA was detectable in 8 of 21 (38.1%) Chronic Phase and 17 of 17 (100%) Acceleration Phase and Blast Crisis, WT1 mRNA was undetected in 1 post-Allo-BMT patient. 39例慢性粒细胞白血病(CML)中慢性期21例有8例阳性(38.;1%25)，加速期和急变期17例均为阳性(100%25)，1例异基因骨髓移植后WT1 mRNA持续为阴性。
METHODS: Polymerase chain reaction (PCR) was used to detect FLT3 gen e expression, and FLT3/ITD gene mutation in 53 CML patients of chronic phase, an d 34 CML patients of accelerated phase or blast crisis. 方法:采用聚合酶链反应(polymerasechainreaction,PCR)检测53例CML慢性期和34例CML加速期或急变期患者DNA水平FLT3基因及FLT3/ITD基因突变。
This study was aimed to investigate the expression and regulation mechanism of DNA-dependent protein kinase catalylic subunit (DNA-PKcs) in chronic myeloid leukemia (CML) and its role in blast crisis of CML. 本研究旨在探讨髓系粒细胞白血病(CML)的DNA依赖蛋白激酶催化亚基(DNA-PKcs)基因表达水平、调控机制及其在CML急性变中的作用。
His strength in a crisis is an ace in the hole. 临危不惧是他的看家本领。
Background and objective:The natural disease history of chronic myeloid leukemia (CML) typicallyevolves 3 distinct clinical stages: chronic phase(CP), accelerated phase and blast crisis. 慢性粒细胞白血病(Chronic myeloid leukemia，CML)的自然病程可分为慢性期(Chronic phase，CP)，加速期(Accelerated phase，CP)和急变期(Blastcrisis phase，BP)。
Methods Five CML patients,2 in chronic phase(CP),1 in accelerated phase(AP) and 2 in blast crisis(BC), all comfirmed the presence of b3a2 bcr/abl mRNA by RT PCR(reverse transcriptase polymerase chain reaction). 方法 5例CML中慢性期 2例 ,加速期 1例 ,急变期 2例 ,均具b3a2型bcr/ablmRNA。
Methods Polymerase chain reaction(PCR)was used to detect FLT3 gene expression in 103 patients of AML cases, 63 ALL, 53 CML in chronic phase, 31 CML in blast crisis, 11 CLL, 36 MDS, 9 MM and 13 NHL with marrow infiltration. 方法采用多聚酶链反应(PCR)方法检测103例初治AML、76例急性淋巴细胞白血病(ALL)、53例慢性粒细胞白血病(CML)慢性期、31例CML急变期、11例慢性淋巴细胞白血病(CLL)、36骨髓增生异常综合征(MDS)患者、9例多发性骨髓瘤(MM)及13例伴有骨髓侵犯非霍奇金淋巴瘤(NHL)患者FLT3基因表达情况。
All of 4 patients with CML at blast crisis stage had positive expresion of WT1. As contrast, neither 10 volunteers, nor 10 patients with no hematological disease were WT1 positive. 16例慢性粒细胞白血病 (CML )中的 7例 (44%25 ) WT1表达阳性 ,其中 4例急变者全部为阳性 ,10名正常人及 10例非血液病患者的 WT1表达阴性。

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