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- biotinylated phosphoramidite 生物素酰亚磷酰胺
- For prolong storage of the biotinylated peptide it is recommended to freeze dry it. 若要延长生物素化单肽的贮存时间,则可以冻干它。
- Objective: To make of high yield protection of purine ribonucleosides for phosphoramidite RNA sythesis. 目的:制备磷酰胺RNA核糖核苷酸嘌呤的高区域保护。
- The advances in application of chiral 2, 2'-binaphthol phosphonite (phosphite, phosphoramidite) in the asymmetric catalysis has been reviewed. 摘要本文综述了手性亚膦(磷)酸联二萘酚酯及手性亚膦(磷)酸联二萘酚胺酯配体在不对称催化应用中的最新进展。
- Methods The neuronal tract tracing by applying HRP(horseradish peroxidase),BDA(biotinylated dextran amine). 方法 HRP和生物素结合的葡聚糖胺(BDA)的神经示踪技术。
- The details of cloning, expression and function of biotinylated luciferase will be discussed in this paper. 本文将就生物素化荧光素酶的克隆、表达以及功能检测进行具体讨论。
- Caruthers's method, known as solid phase phosphoramidite chemistry, is still the basis of most commercial DNA synthesis. 卡拉瑟斯的方法称为固相亚磷酰胺化学法(solidphasephosphoramiditechemistry),是大部份商业用DNA合成仪器的基础。
- In Fig. 3B, avidin protein was printed by photolithography onto a biotinylated PSI surface. 三号乙,抗生物素蛋白印刷光刻到生物防扩散表面。
- The efferent projections of the auditory thalamus in ring dove (Streptopelia risoria) were studied by using the antereograde method of biotinylated dextran amine(BDA). 应用神经示踪物生物素标记的葡聚糖胺对环鸽丘脑听觉中继核团的传出神经投射进行了研究。
- Methods Biotinylated dextranamine (BDA) anterograde tracing and immunofluorescence histochemical staining for histamine/norepinephrine were applied. 方法 生物素化葡聚糖胺(BDA)顺行追踪结合组胺和去甲肾上腺素免疫荧光三标技术。
- Conclusion The GFP-SA bi-functional fusion protein was obtained and could be displayed efficiently on the surface of the biotinylated B16. 此外,GFP-SA双功能融合蛋白的细胞表面修饰对细胞的活力及其生长无显著影响。
- Porcine chondrocytes were biotinylated by using 3-Sulfo-NHS-Biotin, and avidin was immobilized onto material surface by adsorption. 在本研究中提供了一种有效且方便的系统,来增加软骨细胞的贴附量。
- Methods Tetramethyl rhodamine(TMR) or horseradish peroxidase(HRP)retrograde tracing and biotinylated dextran amine(BDA) anterograde tracing techniques were used. 方法四甲基罗达明(TMR)或辣根过氧化物酶(HRP)逆行追踪和生物素葡聚糖胺(BDA)顺行追踪技术。
- The present study examined whether the lumbar spinocerebellar tract projects to the cerebellar nuclei, by using anterograde labeling with biotinylated dextran(BDA). 本实验采用BDA顺行追踪法研究了大鼠腰髓脊髓小脑束向小脑中央核的投射。
- Detailed morphology of developing callosal axons in rat visual and auditory cortices was studied with sensitive anterograde tracer, biotinylated dextran amine (BDA). 用顺行示踪剂 BDA标记研究了发育中大鼠视觉与听觉皮质胼胝体轴突的形态学。
- The marker characteristics of different orientations and states of cellular differentiation of ovarian Brenner tumor were studied by using seven biotinylated lectins. 研究7种生物素化的凝集素对卵巢Brenner瘤不同分化方向和分化性质瘤细胞的标记特点。
- Methods: Biotinylated DNA probe from sorted human chromosome 15 was hybridized to the metaphase spreads of 10 cases of APL.G-banding technique was also used in these samples. 方法:以生物素标记的人类15号染色体特异的DNA为探针,对10例APL患者骨髓中期分裂相进行杂交,所有病例均进行G显带分析。
- Using biotinylated human Y-specificDNA probe PHY2.1 and Cos 84, we carriedout Southern and dot hybridization withhuman DNA from artificially aborted cho-rionic villi. and with DNA of peripherallymphocytes from normal male and female. 作者以流产绒毛和外周血淋巴细胞DNA为材料;通过Southern印迹杂交和点杂交;考察了用生物素标记Y特异DNA探针PHY2.;1和Cos84进行产前性别诊断的可行性。
- oligodeoxynucleoside phosphoramidite 寡脱氧核苷亚磷酰胺
- Methods Horseradish peroxidase(HRP) retrograde tracing combined with biotinylated dextran amine (BDA) anterograde tracing technique was used. The labeling results were observed under light of electron microscope,respectively. 方法 HRP逆行追踪与生物素葡聚糖胺 (BDA)顺行追踪相结合的双标技术 ,标记结果分别在光镜或电镜下观察。
