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- FastStart Taq DNA Polymerase, December ed. 酸外切酶活性（ Roche Package Insert.
- Using chromosome DNA of Lactobacillus casei 34103 as template, thy A (Thymidylate synthase) gene was amplified by PCR with pfu DNA polymerase. 以干酷乳桿菌L.;casei34103染色體DNA為模板，利用PCR技術擴增胸苷酸合成酶(Thymidy-1atesynthase，fhyA)基因，回收純化。
- Telomerase is RNA dependent DNA polymerase, acts to synthesize DNA repeat fragment of telomeres , which is associated with immortalization of cells. 端粒酶是一種RNA依賴的DNA多聚酶,可以合成端粒DNA的重複片段與細胞永生化密切相關。
- First, this term is often used to describe both the viral subtype (A,B,C,D, etc) as well as drug resistance mutations in the HBV DNA polymerase gene. 首先,這個術語經常被用來描述病毒亞型(A,B,C,D等)以及HBV DNA多聚酶基因中出現的耐藥性突變。
- Full-length plasmid DNA was amplified with the above primers with a high-fidelity thermostable DNA polymerase capable of long-rang amplification. 用能擴增長片段的高保真耐熱 DNA聚合酶擴增全長的質粒DNA，直接轉化大腸桿菌。
- Phylogeny within DNA polymerase X family requires a re examination as the expansion of family members,particularly considering the enter of two entomopoxvirus(EPV) members. 隨著DNA聚合酶X家族成員數量的增加 ,特別是兩個昆蟲痘病毒 (entomopoxvirus ,EPV)成員的加入 ,家族內部的系統發育關係需要重新檢查。
- Further studies showed that DNA replication fidelity is decreased and there is an alteration of DNA polymerase spectrum when mammalian cells are attacked by MNNG. 因此，我們推測，哺乳細胞中低保真度的跨損傷DNA聚合酶可能參與了MNNG誘導的哺乳細胞非定標突變。
- A system used for the determination of fidelity of DNA polymerase in PCR was developen in E.coli and was used to determine the fidelity of FD DNA polymerase in PCR amplication. 摘要 在大腸桿菌中建立了一套用於測定 DNA聚合酶在PCR過程中複製精確性的系統,並測定了耐熱FD DNA聚合酶在PCR擴增過程中的複製精確性。
- In molecular biology, processivity is a measure of the average number of nucleotides added by a DNA polymerase enzyme per association/disassociation with the template. 應該說PCR其他的技術層次都已經很完善了，唯一的瓶頸是DNA聚合酶的持續合成能力（processivity）。
- Lamivudine, and most nucleoside analogues, acts by suppression of viral replication through competitive inhibition of reverse transcriptase and DNA polymerase. 拉米還有大多數的核苷類似物，通過競爭性地阻斷翻轉錄酶和DNA聚合酶來抑制病毒複製。
- More specifically, amino acid substitutions occur in the tyrosine-methionine-aspartate- aspartate (YMDD) motif of the DNA polymerase of the virus, which renders the drug much less effective. 更具體的說，病毒DNA聚合酶中的酪氨酸-蛋氨酸-天門冬氨酸-天門冬氨酸（YMDD）基序中發生氨基酸取代，導致藥物失效。
- A gene encoding Trichosanth in (TCS)was amplified by means of DNA polymerase chain reaction(PCR) and cloned from the genomic DNA of Trichosanthes kirilowii. Its DNA sequence has been determined. 本文應用DNA多聚酶鏈式反應（PCR）技術，從栝樓基因組DNA中擴增並克隆了天花粉蛋白（TCS）基因。
- In Q group the gene expressions of DNA polymerase beta, EGFR and c-myc detected by RNA dot blot array were 6.5 folds, 7 folds and 2.2 folds down-regulated respectively, and the wtp53 was 2.5 folds up. Q組的DNAPolp，EGFR及e一mye的RNA斑點TLe掃描數值分別比對照組下調了6.;5倍，7倍，2
- Factors which affect the ISSR analysis of Abies beshanzuensis,such as annealing temperature and concentrations of template DNA,Taq DNA polymerase,Mg~2+ and dNTP,etc. were examined. 以百山祖冷杉DNA為材料;分析了DNA濃度、Mg2+濃度、dNTP濃度、Taq酶的含量以及退火溫度對ISSRPCR擴增結果的影響;經過優化實驗建立了百山祖冷杉ISSRPCR最佳反應體系.
- The results of reverse spot hybridization for both duck hepatitis B virus DNA (DHBV DNA) and special DNA polymerase were compared with those by dot blot for DHBV DNA and electromicroscopy for DHBV particles. 本文將逆向斑點雜交同時檢測鴨乙型肝炎病毒DNA(DHBV DNA)及其特異DNA多聚酶(DNAp)的結果,與斑點雜交檢測DHBV DNA和電鏡檢測DHBV顆粒的結果作了比較。
- METHODS The contents of ET-1 in lung of asthmatic guinea-pigs were measured with radioimmunoassay, and the expressions of ET-1, ETA, ETB and ECEmRNA with reverse transcription DNA polymerase chain reaction (RT-PCR). 方法 放免法測定肺組織ET-1,逆轉錄多聚酶鏈式反應(RT-PCR)方法檢測ET-1、ETA、ETB和ECE的mRNA表達量。
- After the second strand cDNA replacement synthesized, the uneven termini of the double- stranded cDNA were filled in with Puf DNA polymerase and EcoR I adapter were ligated to the blunt ends. cDNA第二鏈合成後用Pfu DNA聚合酶補平腸口I位點，再與EcoR AdaPters連接，經乃。
- Abstract： Lamivudine is a new kind of anti-hepatitis B agent from nucleosides.It inhibits the DNA synthesis of HBV via decreasing the bio-ac tivity of DNA polymerase that depended on RNA. 摘 要： 拉米夫定是新一代核苷類抗乙肝藥物，可通過降低乙型肝炎病毒(HBV)依賴RNA的DNA多聚酶生物活性,明顯抑制HBV-DNA的合成,具有作用強、安全、不良反應少等特點，是治療不同類型的乙型病毒性肝炎的新選擇。
- Many studies show that 4-oxo-dihydroquinolines have good oral bioavailability and plasma clearance,and they suppress both the DNA polymerase of HSV and virus replication in the cells. 多項研究表明,4-氧代二氫喹啉類有良好的口服利用率及中等程度的血漿清除率,不僅對核苷酸聚合酶產生抑制,還可以抑制細胞內的病毒複製。
- Research Advances Baculovirus DNA polymerase gene 桿狀病毒DNA聚合酶基因的研究概況
