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- 農桿菌LBA4404Agrobacterium LBA4404
- 根癌農桿菌LBA4404agrobacterium tumefaciens strain LBA4404
- 針刺后的蝴蝶蘭『White Hikaru』的類原球莖(PLB),與含綠色熒光蛋白基因(gfp)和潮黴素磷酸轉移酶基因(hot)的pcAMBIA 1300-SmGF、P的根瘤農桿菌LBA4404共培養,培育出了轉基因的蝴蝶蘭。Transgenic orchid (Phalaenopsis cv. White Hikaru) plants were regenerated by inoculating a needle wounded protocorm-like body ( PLB) with Agrobacterium tumefaciens strain LBA4404 harboring pCAMBIA 1300-SmGFP that carries green fluorescent protein gene (gfp) and hygromycin phosphotransferase gene ( hpt) .
- 把該反義片段連接至pBI121雙元載體質粒上得到CaMV35S組成型啟動子的表達載體pBI35S-AMF,隨後利用"三親雜交"法將pBI35S-AMF質粒轉入農桿菌LBA4404和EHA105兩個菌株中.The fragment was introduced to pBI121 generating pBI35S-AMF which contained CaMV35S promoter, and then pBI35S-AMF was transferred into Agrobacterium tumefaciens strain LBA4404 and EHA105 through the assistant plasmid pRK2013/HB101 by triparental cross.
- 見難而上
- 農桿菌Agrobacterium tumefaciens
- 根癌土壤農桿菌Ti質粒毒性區VirD1基因的克隆及序列分析Cloning and Sequencing of the VirD 1 Protein Gene Situating in Virus Region of Agribacterium Tumefaciens'Ti Plasmid
- 髮根農桿菌Ri質粒及其在植物次生代謝產物研究中的應用The Ri Plasmid of Agrobacterium rhizogenes and Its Application to Plant Secondary Metabolites
- 農桿菌法Agrobacterium-mediated transformation
- 離體培養植物細胞的表面與農桿菌介導基因轉化頻率的研究Relationship Between the Outer Surfaces of Plant Cells Cultured in vitro and the Frequencies of Transformation Agrobacterium-mediated
- 農桿菌滲入agroinfiltration
- 髮根農桿菌Agrobacterium rhizogenes
- 農桿菌介導Agrobacterium-mediated
- 根瘤農桿菌Agrobacterium tumefaciens
- 農桿菌轉化Agrobacterium-mediated transformation
- 農桿菌注射injection of Agrobacterium
- 殘存農桿菌Agrobacterium
- 放射農桿菌Agrobacterium ractidoacter
- 野生型農桿菌Wild Type Agrobacteria
