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- This CTB gene product was also verified by Western blot analysis. Western blotting结果确认了该条带为CTB基因的产物。
- The results of western blot analysis were in accordance with those of 2-DE. 对部分差异蛋白采用Western-blot方法在蛋白水平的验证结果与2-DE结果基本相符。
- RT-PCR, Western blot analysis and FACS were used to detect the expression of P2X7 receptor in these clones. 荧光分光光度计检测P2X7受体介导的胞内钙离子浓度变化。
- Using morphology observation, the RT-PCR and Western blot analysis to investigate and analysis the sub-type of CnA. 通过病理形态学检查、RT-PCR实验测定及Western blot分析确认CnA亚型。
- The samples were examined delicately to investigated the expression of LeY oligosaccharide by Western blot analysis. 应用Western免疫印迹法,观察Ley寡糖抗原的表达。
- Antigen expression in normal organs and tumor metastases was evaluated by Western blot analysis and flow cytometry. 我们开发并描述了一个临床前的模型,用来评估免疫介导转移性乳腺癌疗法。
- The lev- els of Bcl-2 and Bcl-X_L proteins were examined by western blot analysis. Western blot法分析细胞内Bcl-2和Bcl-X_L蛋白水平的变化。
- Myocardium osteopontin protein expression level in non-infarcted myocardium was detected with Western blot analysis. 组织学方法检测非梗死区胶原纤维沉积和心肌细胞横径;
- The expression of phosphorated Erk1/2 protein in MGC-803 cells was examined using Western blot analysis. EGCG处理后免疫细胞化学检测c-Myc蛋白的表达下调,免疫印迹检测磷酸化Erk1/2蛋白表达下调。
- With Western blot analysis, the expected 100-kDa RTN3-A1 protein was detected in mouse brain. 用Western杂交的方法,我检测到了预期的100 kDa 的鼠RTN3-A1在脑组织的表过。
- SDS-PAGE and Western blot analysis using the infection cells showed that the band of 25 kD was particular to pGH gene product. 感染重组病毒的细胞可溶性蛋白SDS PAGE和Western blot分析结果显示 ,感染细胞蛋白电泳带的 2 5kD处有 1条猪生长激素特异带。
- The results are shown as follows:1,Western blot analysis confirmed that BAF complex and NF1/CTF exist in the nuclei of self-proliferative Jurkat cells. 通过免疫印记实验证实,在自主增殖的Jurkat细胞核中,存在BAF复合物和转录因子NF1/CTF。
- Western blot analysis was used to detect pl85 HER-2, HER-2 ECD and phospho-HER-2. Two-site ELISA assay was used for the detection of HER-2 ECD. 双抗夹心ELISA法检测细胞培养上清中HER-2 ECD水平,Western blot检测细胞HER-2蛋白水平。
- The expressed BMP-4 was further identified to be a 43,000 dalton molecular band with anti-hBMP4 antibody in western blot analysis. 实验结果表明,表达产物经蛋白质免疫印迹和定量点杂交分析证实表达产物具有良好的抗原性和特异性,在蛋白电泳分析结果发现表达的hBMP4的蛋白分子量为43,000 dalton。
- The titer of mAb was detected by ELISA, specificity of mAb by Western blot analysis and antigen tissue localization by immunohistochemical staining. 用间接ELISA法测定mAb效价、Western blot鉴定抗体特异性、免疫组化染色法对抗体相应的抗原进行组织定位。
- Then the recombinant plasmid was transfected into the COS7 cell line and identified its expressing of protein by Western blot analysis. 以脂质体法将pcDNA3.;1(+)-flk1-domainl-7转染COS7细胞;用Western blot方法对目的基因蛋白质的表达进行鉴定。
- Brain heme oxygenase-1, transferrin, transferrin receptor and ferritin were examined by Western blot analysis and immunohistochemistry. 脑血红素氧合酶-1、转铁蛋白、转铁蛋白受体和铁蛋白应用蛋白质印迹分析和免疫组织化学方法检测。
- Northern and Western blot analysis in pMR-FG1 and pBI-FG1transformants showed that pMR-FG1 can improve the gene expression both intranscription level and in translation level. 对部分 pMR-FG1 和 pBI-FG1 转化植株的 Northern blot 和 Westernblot 分析证明,pMR-FG1 转化植株中融合蛋白的表达在转录水平和翻译水平明显高于 pBI-FG1 转化植株。
- Compared with pcDNA3 vector,there was significant increase of peroxiredoxin 1 expression in the PC3 cells stably transfected with pcDNA3/Prx 1 monitored by Western blot analysis. Western blot分析表明,Prx 1真核表达质粒稳定转染的PC3细胞表达Prx 1显著增加;
- Western blot analysis showed that the band density areas of HS,CUR and PB groups were higher than those in NS and DMSO groups, especially in CUR group ( P < 0.01) . Western印迹分析经图像处理仪扫描显示CUR组、HS组、PB组高于NS组及DMSO组 ;其中以CUR组最高 (P <0 0 1)。
