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- Methods: Using Western blotting hybridization assay, we detected VMLC1, VMLC2 in 13 patients with RHD and 7 cases of accidental deathes. 方法:采用West-ernbloting技术对13例RHD心衰患者及7例意外死亡者VMLC-1,VMLC-2含量进行定量分析。
- Effect of labeling was detected by dot blot hybridization. 点杂交方法检测探针标记效果。
- This CTB gene product was also verified by Western blot analysis. Western blotting结果确认了该条带为CTB基因的产物。
- Western blot showed 60 kDa, which may be posttranslated product. Western blot 结果显示,该基因的真核表达产物分子量约为60 kD,可能是翻译后修饰的结果。
- We detected 213 specimens from 173 people using probe of pBR-322 by Dot and Southern blot hybridization. 我们用常见的克隆载体pBR322作探针,用核酸点杂交和Southern吸印方法,检测了来自173人的213例样品。
- Expression of the M 2-1 gene was examined by RT PCR and Western blot. 重组真核质粒PXJ 4 1/M2 -1转染肺腺癌PAa细胞 ,应用RT PCR、Westernblot方法检测表达。
- Objective: To study the clinical value of detecting mycobacterium tuberculosis by dot blot hybridization. 摘要目的:探讨斑点杂交法检测结核分枝杆菌的临床应用价值。
- The results of western blot analysis were in accordance with those of 2-DE. 对部分差异蛋白采用Western-blot方法在蛋白水平的验证结果与2-DE结果基本相符。
- From recombinant plasmid pGEMTH2 prepared cRNA probe was identified by dot blot hybridization. 使用斑点杂交证实我们制备的探针是敏感而可靠的。
- Western blot was used to analyze a panel of HSP90 client proteins expression. Western blot分析药物对细胞通路信号蛋白及细胞周期相关蛋白的表达;
- The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%. PCR-膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.;49%25;特异度为100%25。
- To develop a universal PCR-reverse blot hybridization assay, which aime to detect and identify pathogenic yeast rapidly and exactly. 摘要建立PCR结合寡核苷酸探针反向斑点杂交技术,快速检测及鉴定致病性酵母菌。
- Methods:Polymerase chain reaction in combination with dot blot hybridization of allele specific oligonucleotide(PCR/ASO) probes was used. 方法:采用聚合酶链反应结合等位基因特异的寡核苷酸探针杂交(PCR/ASO)技术。
- Methods:Using digoxigenin-labeled probe,75 sera of known or unknown etiology, and 17 blood donors were detected by dot blot hybridization. 方法:用地高辛素标记基因探针,斑点杂交法检测未知和已知病原的肝炎患者75例,正常对照17例。
- Methods 2H4 and 5D3 were used to immunize mice. Ab3 in anti sera were tested by ELISA and Western blot. 方法用Ab2免疫Balb/c小鼠获得抗血清 ,以ELISA检测其Ab3,Westernblot分析其识别抗原的分子质量。
- Expression of messenger endothelin 1 (ET 1) RNAs and its receptor subunits (ETA,ETB)were studied by northern blot hybridization in rat kidney following renal ischemia reperfusion. 为了探究内皮素?1(ET?1)对肾功能的影响和作用方式,采用斑点杂交和原位杂交方法对大鼠缺血60分钟再灌注肾组织ET?1及其受体亚型(ETA、ETB)的基因表达进行了研究。
- HIV test. If the initial test is positive, please repeat and perform Western Blot. 艾滋病病毒检验,如果最初的检验是呈阳性,请进行再次检验。
- Methods: The gene rearrangement in blood and bone marrow of 12 children with AML was detected by Southern blot hybridization using LE11. 8, MYO and M13 probes. 方法:用LE11.;8、MYO和M13探针,经Southern杂交法检测12例儿童急性粒细胞白血病患者的外周血或骨髓细胞的基因重排。
- RT-PCR and Western blot shown that there was a decrease in the expression of BRCP mRNA and protein in SWt cells. RT-PCR和Western印迹显示,转染细胞中BCRP基因mRNA和蛋白质的表达下降甚至消失。